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Title: Crystal structure of the human sterol transporter ABCG5/ABCG8

Abstract

ATP binding cassette (ABC) transporters play critical roles in maintaining sterol balance in higher eukaryotes. The ABCG5/ABCG8 heterodimer (G5G8) mediates excretion of neutral sterols in liver and intestines. Mutations disrupting G5G8 cause sitosterolaemia, a disorder characterized by sterol accumulation and premature atherosclerosis. Here we use crystallization in lipid bilayers to determine the X-ray structure of human G5G8 in a nucleotide-free state at 3.9 Å resolution, generating the first atomic model of an ABC sterol transporter. The structure reveals a new transmembrane fold that is present in a large and functionally diverse superfamily of ABC transporters. Here, the transmembrane domains are coupled to the nucleotide-binding sites by networks of interactions that differ between the active and inactive ATPases, reflecting the catalytic asymmetry of the transporter. The G5G8 structure provides a mechanistic framework for understanding sterol transport and the disruptive effects of mutations causing sitosterolaemia.

Authors:
 [1];  [1];  [1];  [1];  [1];  [2];  [1];  [1];  [1];  [1];  [1];  [1]
+ Show Author Affiliations
  1. Univ. of Texas Southwestern Medical Center, Dallas, TX (United States)
  2. Texas Tech Univ. Health Sciences Center, Lubbock, TX (United States)
Publication Date:
Research Org.:
Argonne National Laboratory (ANL), Argonne, IL (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES). Scientific User Facilities Division; American Heart Association South Central Affiliate; American Heart Association Texas Affiliate; Welch Foundation; Packard Foundation; Howard Hughes Medical Inst.; National Inst. of Health
OSTI Identifier:
1255303
Grant/Contract Number:  
AC02-06CH11357; 0825285F; 0463130Y; I-1770; HL72304; P01-HL20948; GM094575; GM053163; GM117080; GM113050
Resource Type:
Accepted Manuscript
Journal Name:
Nature (London)
Additional Journal Information:
Journal Name: Nature (London); Journal Volume: 533; Journal Issue: 7604; Journal ID: ISSN 0028-0836
Publisher:
Nature Publishing Group
Country of Publication:
United States
Language:
ENGLISH
Subject:
59 BASIC BIOLOGICAL SCIENCES; Transporters; X-ray crystallography

Citation Formats

Lee, Jyh-Yeuan, Kinch, Lisa N., Borek, Dominika M., Wang, Jin, Wang, Junmei, Urbatsch, Ina L., Xie, Xiao-Song, Grishin, Nikolai V., Cohen, Jonathan C., Otwinowski, Zbyszek, Hobbs, Helen H., and Rosenbaum, Daniel M. Crystal structure of the human sterol transporter ABCG5/ABCG8. United States: N. p., 2016. Web. doi:10.1038/nature17666.
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Lee, Jyh-Yeuan, Kinch, Lisa N., Borek, Dominika M., Wang, Jin, Wang, Junmei, Urbatsch, Ina L., Xie, Xiao-Song, Grishin, Nikolai V., Cohen, Jonathan C., Otwinowski, Zbyszek, Hobbs, Helen H., & Rosenbaum, Daniel M. Crystal structure of the human sterol transporter ABCG5/ABCG8. United States. https://doi.org/10.1038/nature17666
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Lee, Jyh-Yeuan, Kinch, Lisa N., Borek, Dominika M., Wang, Jin, Wang, Junmei, Urbatsch, Ina L., Xie, Xiao-Song, Grishin, Nikolai V., Cohen, Jonathan C., Otwinowski, Zbyszek, Hobbs, Helen H., and Rosenbaum, Daniel M. Wed . "Crystal structure of the human sterol transporter ABCG5/ABCG8". United States. https://doi.org/10.1038/nature17666. https://www.osti.gov/servlets/purl/1255303.
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@article{osti_1255303,
title = {Crystal structure of the human sterol transporter ABCG5/ABCG8},
author = {Lee, Jyh-Yeuan and Kinch, Lisa N. and Borek, Dominika M. and Wang, Jin and Wang, Junmei and Urbatsch, Ina L. and Xie, Xiao-Song and Grishin, Nikolai V. and Cohen, Jonathan C. and Otwinowski, Zbyszek and Hobbs, Helen H. and Rosenbaum, Daniel M.},
abstractNote = {ATP binding cassette (ABC) transporters play critical roles in maintaining sterol balance in higher eukaryotes. The ABCG5/ABCG8 heterodimer (G5G8) mediates excretion of neutral sterols in liver and intestines. Mutations disrupting G5G8 cause sitosterolaemia, a disorder characterized by sterol accumulation and premature atherosclerosis. Here we use crystallization in lipid bilayers to determine the X-ray structure of human G5G8 in a nucleotide-free state at 3.9 Å resolution, generating the first atomic model of an ABC sterol transporter. The structure reveals a new transmembrane fold that is present in a large and functionally diverse superfamily of ABC transporters. Here, the transmembrane domains are coupled to the nucleotide-binding sites by networks of interactions that differ between the active and inactive ATPases, reflecting the catalytic asymmetry of the transporter. The G5G8 structure provides a mechanistic framework for understanding sterol transport and the disruptive effects of mutations causing sitosterolaemia.},
doi = {10.1038/nature17666},
journal = {Nature (London)},
number = 7604,
volume = 533,
place = {United States},
year = {Wed May 04 00:00:00 EDT 2016},
month = {Wed May 04 00:00:00 EDT 2016}
}
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Works referenced in this record:

Evaluating the Performance of the ff99SB Force Field Based on NMR Scalar Coupling Data
journal, August 2009

Refinement of Macromolecular Structures by the Maximum-Likelihood Method
journal, May 1997

  • Murshudov, G. N.; Vagin, A. A.; Dodson, E. J.
  • Acta Crystallographica Section D Biological Crystallography, Vol. 53, Issue 3
  • DOI: 10.1107/S0907444996012255

Overview of the CCP 4 suite and current developments
journal, March 2011

  • Winn, Martyn D.; Ballard, Charles C.; Cowtan, Kevin D.
  • Acta Crystallographica Section D Biological Crystallography, Vol. 67, Issue 4
  • DOI: 10.1107/S0907444910045749

Carbon catabolite repression of the maltose transporter revealed by X-ray crystallography
journal, June 2013

  • Chen, Shanshuang; Oldham, Michael L.; Davidson, Amy L.
  • Nature, Vol. 499, Issue 7458
  • DOI: 10.1038/nature12232

Prediction of CASP6 structures using automated robetta protocols
journal, January 2005

  • Chivian, Dylan; Kim, David E.; Malmström, Lars
  • Proteins: Structure, Function, and Bioinformatics, Vol. 61, Issue S7
  • DOI: 10.1002/prot.20733

Automatic atom type and bond type perception in molecular mechanical calculations
journal, October 2006

  • Wang, Junmei; Wang, Wei; Kollman, Peter A.
  • Journal of Molecular Graphics and Modelling, Vol. 25, Issue 2
  • DOI: 10.1016/j.jmgm.2005.12.005

Comparison of simple potential functions for simulating liquid water
journal, July 1983

  • Jorgensen, William L.; Chandrasekhar, Jayaraman; Madura, Jeffry D.
  • The Journal of Chemical Physics, Vol. 79, Issue 2
  • DOI: 10.1063/1.445869

TransportDB: a comprehensive database resource for cytoplasmic membrane transport systems and outer membrane channels
journal, January 2007

  • Ren, Q.; Chen, K.; Paulsen, I. T.
  • Nucleic Acids Research, Vol. 35, Issue Database
  • DOI: 10.1093/nar/gkl925

MolProbity: all-atom contacts and structure validation for proteins and nucleic acids
journal, May 2007

  • Davis, I. W.; Leaver-Fay, A.; Chen, V. B.
  • Nucleic Acids Research, Vol. 35, Issue Web Server
  • DOI: 10.1093/nar/gkm216

GPCR Engineering Yields High-Resolution Structural Insights into  2-Adrenergic Receptor Function
journal, October 2007

Lipid14: The Amber Lipid Force Field
journal, January 2014

  • Dickson, Callum J.; Madej, Benjamin D.; Skjevik, Åge A.
  • Journal of Chemical Theory and Computation, Vol. 10, Issue 2
  • DOI: 10.1021/ct4010307

CLANS: a Java application for visualizing protein families based on pairwise similarity
journal, July 2004

AL2CO: calculation of positional conservation in a protein sequence alignment
journal, August 2001

Synchronization of trajectories in canonical molecular-dynamics simulations: Observation, explanation, and exploitation
journal, April 2004

  • Uberuaga, Blas P.; Anghel, Marian; Voter, Arthur F.
  • The Journal of Chemical Physics, Vol. 120, Issue 14
  • DOI: 10.1063/1.1667473

CMView: Interactive contact map visualization and analysis
journal, April 2011

Disruption of Abcg5 and Abcg8 in mice reveals their crucial role in biliary cholesterol secretion
journal, November 2002

  • Yu, L.; Hammer, R. E.; Li-Hawkins, J.
  • Proceedings of the National Academy of Sciences, Vol. 99, Issue 25
  • DOI: 10.1073/pnas.252582399

Diffraction data analysis in the presence of radiation damage
journal, March 2010

  • Borek, Dominika; Cymborowski, Marcin; Machius, Mischa
  • Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 4
  • DOI: 10.1107/S0907444909040177

HKL -3000: the integration of data reduction and structure solution – from diffraction images to an initial model in minutes
journal, July 2006

  • Minor, Wladek; Cymborowski, Marcin; Otwinowski, Zbyszek
  • Acta Crystallographica Section D Biological Crystallography, Vol. 62, Issue 8
  • DOI: 10.1107/S0907444906019949

Coexpression of ATP-binding cassette proteins ABCG5 and ABCG8 permits their transport to the apical surface
journal, September 2002

  • Graf, Gregory A.; Li, Wei-Ping; Gerard, Robert D.
  • Journal of Clinical Investigation, Vol. 110, Issue 5
  • DOI: 10.1172/JCI0216000

Structure and mechanism of ABC transporter proteins
journal, August 2007

  • Hollenstein, Kaspar; Dawson, Roger JP; Locher, Kaspar P.
  • Current Opinion in Structural Biology, Vol. 17, Issue 4
  • DOI: 10.1016/j.sbi.2007.07.003

Mutations in the white gene of Drosophila melanogaster affecting ABC transporters that determine eye colouration
journal, July 1999

  • Mackenzie, Susan M.; Brooker, Michael R.; Gill, Timothy R.
  • Biochimica et Biophysica Acta (BBA) - Biomembranes, Vol. 1419, Issue 2
  • DOI: 10.1016/S0005-2736(99)00064-4

The Human ATP-Binding Cassette (ABC) Transporter Superfamily
journal, January 2001

Experimental phasing with SHELXC / D / E : combining chain tracing with density modification
journal, March 2010

  • Sheldrick, George M.
  • Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 4
  • DOI: 10.1107/S0907444909038360

Bile Acids Stimulate ATP Hydrolysis in the Purified Cholesterol Transporter ABCG5/G8
journal, April 2010

  • Johnson, Brandy J. Harvey; Lee, Jyh-Yeuan; Pickert, Amanda
  • Biochemistry, Vol. 49, Issue 16
  • DOI: 10.1021/bi902064g

[20] Processing of X-ray diffraction data collected in oscillation mode
book, January 1997

MAFFT: Iterative Refinement and Additional Methods
book, January 2013

Crystallizing Membrane Proteins for Structure Determination: Use of Lipidic Mesophases
journal, June 2009

Structure of an ABC transporter in complex with its binding protein
journal, February 2007

  • Hollenstein, Kaspar; Frei, Dominik C.; Locher, Kaspar P.
  • Nature, Vol. 446, Issue 7132
  • DOI: 10.1038/nature05626

Functional Asymmetry of Nucleotide-binding Domains in ABCG5 and ABCG8
journal, January 2006

The Amber biomolecular simulation programs
journal, January 2005

  • Case, David A.; Cheatham, Thomas E.; Darden, Tom
  • Journal of Computational Chemistry, Vol. 26, Issue 16
  • DOI: 10.1002/jcc.20290

Crystallization of bacteriorhodopsin from bicelle formulations at room temperature
journal, March 2005

The oligomeric structures of plant cryptochromes
journal, May 2020

ABCG5 and ABCG8 Are Obligate Heterodimers for Protein Trafficking and Biliary Cholesterol Excretion
journal, September 2003

  • Graf, Gregory A.; Yu, Liqing; Li, Wei-Ping
  • Journal of Biological Chemistry, Vol. 278, Issue 48
  • DOI: 10.1074/jbc.M310223200

Structures of P-glycoprotein reveal its conformational flexibility and an epitope on the nucleotide-binding domain
journal, July 2013

  • Ward, A. B.; Szewczyk, P.; Grimard, V.
  • Proceedings of the National Academy of Sciences, Vol. 110, Issue 33
  • DOI: 10.1073/pnas.1309275110

ABC transporter research: going strong 40 years on
journal, October 2015

  • Theodoulou, Frederica L.; Kerr, Ian D.
  • Biochemical Society Transactions, Vol. 43, Issue 5
  • DOI: 10.1042/BST20150139

Numerical integration of the cartesian equations of motion of a system with constraints: molecular dynamics of n-alkanes
journal, March 1977

  • Ryckaert, Jean-Paul; Ciccotti, Giovanni; Berendsen, Herman J. C.
  • Journal of Computational Physics, Vol. 23, Issue 3
  • DOI: 10.1016/0021-9991(77)90098-5

Assessing the utility of coevolution-based residue-residue contact predictions in a sequence- and structure-rich era
journal, September 2013

  • Kamisetty, H.; Ovchinnikov, S.; Baker, D.
  • Proceedings of the National Academy of Sciences, Vol. 110, Issue 39
  • DOI: 10.1073/pnas.1314045110

Transmembrane heme delivery systems
journal, April 1998

  • Goldman, B. S.; Beck, D. L.; Monika, E. M.
  • Proceedings of the National Academy of Sciences, Vol. 95, Issue 9
  • DOI: 10.1073/pnas.95.9.5003

Expression of 25 Human ABC Transporters in the Yeast Pichia pastoris and Characterization of the Purified ABCC3 ATPase Activity †
journal, July 2007

  • Chloupková, Maja; Pickert, Amanda; Lee, Jyh-Yeuan
  • Biochemistry, Vol. 46, Issue 27
  • DOI: 10.1021/bi700020m

Crystal structures of a polypeptide processing and secretion transporter
journal, July 2015

  • Lin, David Yin-wei; Huang, Shuo; Chen, Jue
  • Nature, Vol. 523, Issue 7561
  • DOI: 10.1038/nature14623

Genome sequence of Streptococcus mutans UA159, a cariogenic dental pathogen
journal, October 2002

  • Ajdic, D.; McShan, W. M.; McLaughlin, R. E.
  • Proceedings of the National Academy of Sciences, Vol. 99, Issue 22
  • DOI: 10.1073/pnas.172501299

Large-scale determination of previously unsolved protein structures using evolutionary information
journal, September 2015

A well-behaved electrostatic potential based method using charge restraints for deriving atomic charges: the RESP model
journal, October 1993

  • Bayly, Christopher I.; Cieplak, Piotr; Cornell, Wendy
  • The Journal of Physical Chemistry, Vol. 97, Issue 40
  • DOI: 10.1021/j100142a004

Crystal Structure of the Maltose Transporter in a Pretranslocation Intermediate State
journal, May 2011

Coexpression of ATP-binding cassette proteins ABCG5 and ABCG8 permits their transport to the apical surface
journal, September 2002

  • Graf, Gregory A.; Li, Wei-Ping; Gerard, Robert D.
  • Journal of Clinical Investigation, Vol. 110, Issue 5
  • DOI: 10.1172/jci200216000

Towards an accurate representation of electrostatics in classical force fields: Efficient implementation of multipolar interactions in biomolecular simulations
journal, January 2004

  • Sagui, Celeste; Pedersen, Lee G.; Darden, Thomas A.
  • The Journal of Chemical Physics, Vol. 120, Issue 1
  • DOI: 10.1063/1.1630791

The Buccaneer software for automated model building. 1. Tracing protein chains
journal, August 2006

PARP1 exhibits enhanced association and catalytic efficiency with γH2A.X-nucleosome
journal, December 2019

Crystal structure of a heterodimeric ABC transporter in its inward-facing conformation
journal, March 2012

  • Hohl, Michael; Briand, Christophe; Grütter, Markus G.
  • Nature Structural & Molecular Biology, Vol. 19, Issue 4
  • DOI: 10.1038/nsmb.2267

Normal Mode Analysis of Biomolecular Structures: Functional Mechanisms of Membrane Proteins
journal, March 2010

  • Bahar, Ivet; Lezon, Timothy R.; Bakan, Ahmet
  • Chemical Reviews, Vol. 110, Issue 3
  • DOI: 10.1021/cr900095e

Features and development of Coot
journal, March 2010

  • Emsley, P.; Lohkamp, B.; Scott, W. G.
  • Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 4
  • DOI: 10.1107/S0907444910007493

Sex Limited Inheritance in Drosophila
journal, July 1910

Membrane Porters of ATP-Binding Cassette Transport Systems Are Polyphyletic
journal, September 2009

Sequences in the Nonconsensus Nucleotide-binding Domain of ABCG5/ABCG8 Required for Sterol Transport
journal, January 2011

  • Wang, Jin; Grishin, Nick; Kinch, Lisa
  • Journal of Biological Chemistry, Vol. 286, Issue 9
  • DOI: 10.1074/jbc.M110.210880

Measurement errors and their consequences in protein crystallography
journal, October 2003

  • Borek, Dominika; Minor, Wladek; Otwinowski, Zbyszek
  • Acta Crystallographica Section D Biological Crystallography, Vol. 59, Issue 11
  • DOI: 10.1107/S0907444903020924

Relative roles of ABCG5/ABCG8 in liver and intestine
journal, November 2014

  • Wang, Jin; Mitsche, Matthew A.; Lütjohann, Dieter
  • Journal of Lipid Research, Vol. 56, Issue 2
  • DOI: 10.1194/jlr.M054544

Robust and accurate prediction of residue–residue interactions across protein interfaces using evolutionary information
journal, May 2014

  • Ovchinnikov, Sergey; Kamisetty, Hetunandan; Baker, David
  • eLife, Vol. 3
  • DOI: 10.7554/eLife.02030

Asymmetry in the Structure of the ABC Transporter-Binding Protein Complex BtuCD-BtuF
journal, September 2007

Multiparametric scaling of diffraction intensities
journal, April 2003

  • Otwinowski, Zbyszek; Borek, Dominika; Majewski, Wladyslaw
  • Acta Crystallographica Section A Foundations of Crystallography, Vol. 59, Issue 3
  • DOI: 10.1107/S0108767303005488

Identification of a gene, ABCG5, important in the regulation of dietary cholesterol absorption
journal, January 2001

  • Lee, Mi-Hye; Lu, Kangmo; Hazard, Star
  • Nature Genetics, Vol. 27, Issue 1
  • DOI: 10.1038/83799

Inhibition of clathrin-coated vesicle acidification by duramycin.
journal, March 1984

MolProbity: all-atom contacts and structure validation for proteins and nucleic acids
text, January 2007

  • B., Chen, Vincent; C., Richardson, David; S., Richardson, Jane
  • The University of North Carolina at Chapel Hill University Libraries
  • DOI: 10.17615/m2an-p252

Crystal structure of a heterodimeric ABC transporter in its inward-facing conformation
text, January 2012

  • Hohl, Michael; Briand, Christophe; Grütter, Markus G.
  • Nature Publishing Group
  • DOI: 10.5167/uzh-65059

Pathogenomic Sequence Analysis of Bacillus cereus and Bacillus thuringiensis Isolates Closely Related to Bacillus anthracis
journal, November 2006

  • Han, Cliff S.; Xie, Gary; Challacombe, Jean F.
  • Journal of Bacteriology, Vol. 188, Issue 21
  • DOI: 10.1128/jb.01430-06

Overview of the CCP4 suite and current developments.
text, January 2011

  • Winn, Martyn D.; Ballard, Charles C.; Cowtan, Kevin D.
  • Apollo - University of Cambridge Repository
  • DOI: 10.17863/cam.52322

Structures of P-glycoprotein reveal its conformational flexibility and an epitope on the nucleotide-binding domain
text, January 2013

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    journal, February 2019

    • Nakano, Takanari; Inoue, Ikuo; Murakoshi, Takayuki
    • Nutrients, Vol. 11, Issue 2
    • DOI: 10.3390/nu11020310

    The structure of the human ABC transporter ABCG2 reveals a novel mechanism for drug extrusion
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    Antibiotic Resistance Mediated by the MacB ABC Transporter Family: A Structural and Functional Perspective
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    journal, December 2018

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    • DOI: 10.1042/bst20180269

    Learning the ABCs one at a time: structure and mechanism of ABC transporters
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    • Biochemical Society Transactions, Vol. 47, Issue 1
    • DOI: 10.1042/bst20180147

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    journal, December 2018

    • Wright, Jack; Muench, Stephen P.; Goldman, Adrian
    • Biochemical Society Transactions, Vol. 46, Issue 6
    • DOI: 10.1042/bst20180146

    In vitro NTPase activity of highly purified Pdr5, a major yeast ABC multidrug transporter
    journal, May 2019

    Structural basis of small-molecule inhibition of human multidrug transporter ABCG2
    journal, April 2018

    • Jackson, Scott M.; Manolaridis, Ioannis; Kowal, Julia
    • Nature Structural & Molecular Biology, Vol. 25, Issue 4
    • DOI: 10.1038/s41594-018-0049-1

    Inferring the Evolutionary History of Your Favorite Protein: A Guide for Molecular Biologists
    journal, April 2019

    • Hooff, Jolien J. E.; Tromer, Eelco; Dam, Teunis J. P.
    • BioEssays, Vol. 41, Issue 5
    • DOI: 10.1002/bies.201900006

    Residues contributing to drug transport by ABCG2 are localised to multiple drug-binding pockets
    journal, May 2018

    • Cox, Megan H.; Kapoor, Parth; Briggs, Deborah A.
    • Biochemical Journal, Vol. 475, Issue 9
    • DOI: 10.1042/bcj20170923

    The ABCG2 multidrug transporter is a pump gated by a valve and an extracellular lid
    journal, November 2019

    • Khunweeraphong, Narakorn; Szöllősi, Daniel; Stockner, Thomas
    • Nature Communications, Vol. 10, Issue 1
    • DOI: 10.1038/s41467-019-13302-2

    Cryo-EM structures of a human ABCG2 mutant trapped in ATP-bound and substrate-bound states
    journal, November 2018

    • Manolaridis, Ioannis; Jackson, Scott M.; Taylor, Nicholas M. I.
    • Nature, Vol. 563, Issue 7731
    • DOI: 10.1038/s41586-018-0680-3

    Significance of Cholesterol-Binding Motifs in ABCA1, ABCG1, and SR-B1 Structure
    journal, December 2018

    • Dergunov, Alexander D.; Savushkin, Eugeny V.; Dergunova, Liudmila V.
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    journal, June 2019

    Crystal structure of tripartite-type ABC transporter MacB from Acinetobacter baumannii.
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    • Okada, Ui; Yamashita, Eiki; Neuberger, Arthur
    • Springer Science and Business Media LLC
    • DOI: 10.17863/cam.17993

    Mechanisms and regulation of cholesterol homeostasis
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    • Nature Reviews Molecular Cell Biology, Vol. 21, Issue 4
    • DOI: 10.1038/s41580-019-0190-7

    Pharmacogenetic Aspects of the Interaction of AT1 Receptor Antagonists With ATP-Binding Cassette Transporter ABCG2
    journal, May 2018

    Split tasks of asymmetric nucleotide‐binding sites in the heterodimeric ABC exporter EfrCD
    journal, April 2017

    • Hürlimann, Lea M.; Hohl, Michael; Seeger, Markus A.
    • The FEBS Journal, Vol. 284, Issue 11
    • DOI: 10.1111/febs.14065

    ABCG5/G8: a structural view to pathophysiology of the hepatobiliary cholesterol secretion
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    • Zein, Aiman A.; Kaur, Rupinder; Hussein, Toka O. K.
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    • Molinski, Steven V.; Bozóky, Zoltán; Iram, Surtaj H.
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    • DOI: 10.1155/2017/1529402

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    • Escolà-Gil, Joan Carles; Quesada, Helena; Julve, Josep
    • Current Atherosclerosis Reports, Vol. 16, Issue 7
    • DOI: 10.1007/s11883-014-0424-2

    Crystal structure of tripartite-type ABC transporter MacB from Acinetobacter baumannii
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    • Bountra, Kiran; Hagelueken, Gregor; Choudhury, Hassanul G.
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    • DOI: 10.15252/embj.201797278

    Genes Potentially Associated with Familial Hypercholesterolemia
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    • Mikhailova, Svetlana; Ivanoshchuk, Dinara; Timoshchenko, Olga
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    • Caffalette, Christopher A.; Corey, Robin A.; Sansom, Mark S. P.
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    • Taylor, Nicholas M. I.; Manolaridis, Ioannis; Jackson, Scott M.
    • Nature, Vol. 546, Issue 7659
    • DOI: 10.1038/nature22345

    Expression of multidrug transporter P‐glycoprotein in Pichia pastoris affects the host's methanol metabolism
    journal, May 2019

    PBDE-47 and PBDE mixture (DE-71) toxicities and liver transcriptomic changes at PND 22 after in utero/postnatal exposure in the rat
    journal, September 2018

    Application of High-Throughput Sequencing in the Diagnosis of Inherited Thrombocytopenia
    journal, July 2018

    • Wang, Qi; Cao, Lijuan; Sheng, Guangying
    • Clinical and Applied Thrombosis/Hemostasis, Vol. 24, Issue 9_suppl
    • DOI: 10.1177/1076029618790696

    Macrophage-derived IL-1β/NF-κB signaling mediates parenteral nutrition-associated cholestasis
    journal, April 2018

    Architecture of a channel-forming O-antigen polysaccharide ABC transporter
    journal, January 2018

    • Bi, Yunchen; Mann, Evan; Whitfield, Chris
    • Nature, Vol. 553, Issue 7688
    • DOI: 10.1038/nature25190

    Antibiotic Resistance Mediated by the MacB ABC Transporter Family: A Structural and Functional Perspective.
    text, January 2018

    • Greene, Nicholas; Kaplan, Elise; Crow, Allister
    • Apollo - University of Cambridge Repository
    • DOI: 10.17863/cam.26693

    Structural Biology and Structure–Function Relationships of Membrane Proteins
    journal, March 2021

    Structural basis of small-molecule inhibition of human multidrug transporter ABCG2
    text, January 2018

    Quantitative comparison of ABC membrane protein type I exporter structures in a standardized way
    journal, January 2018

    • Csizmadia, Georgina; Farkas, Bianka; Spagina, Zoltán
    • Computational and Structural Biotechnology Journal, Vol. 16
    • DOI: 10.1016/j.csbj.2018.10.008

    Architecture of a channel-forming O-antigen polysaccharide ABC transporter
    journal, January 2018

    • Bi, Yunchen; Mann, Evan; Whitfield, Chris
    • Nature, Vol. 553, Issue 7688
    • DOI: 10.1038/nature25190

    Structure of the MacAB–TolC ABC-type tripartite multidrug efflux pump
    journal, May 2017

    • Fitzpatrick, Anthony W. P.; Llabrés, Salomé; Neuberger, Arthur
    • Nature Microbiology, Vol. 2, Issue 7
    • DOI: 10.1038/nmicrobiol.2017.70

    Macrophage-derived IL-1β/NF-κB signaling mediates parenteral nutrition-associated cholestasis
    journal, April 2018

    A lipid gating mechanism for the channel-forming O antigen ABC transporter
    journal, February 2019

    • Caffalette, Christopher A.; Corey, Robin A.; Sansom, Mark S. P.
    • Nature Communications, Vol. 10, Issue 1
    • DOI: 10.1038/s41467-019-08646-8

    Expression and function of Abcg4 in the mouse blood-brain barrier: role in restricting the brain entry of amyloid-β peptide
    journal, October 2017

    Structure-function relationships in ABCG2: insights from molecular dynamics simulations and molecular docking studies
    journal, November 2017

    • Ferreira, Ricardo J.; Bonito, Cátia A.; Cordeiro, M. Natália D. S.
    • Scientific Reports, Vol. 7, Issue 1
    • DOI: 10.1038/s41598-017-15452-z

    ABCG5/G8: a structural view to pathophysiology of the hepatobiliary cholesterol secretion
    journal, October 2019

    • Zein, Aiman A.; Kaur, Rupinder; Hussein, Toka O. K.
    • Biochemical Society Transactions, Vol. 47, Issue 5
    • DOI: 10.1042/bst20190130

    Expression of multidrug transporter P‐glycoprotein in Pichia pastoris affects the host's methanol metabolism
    journal, May 2019

    3D structure of the transporter ABCG2—What's new?
    journal, February 2020

    • Eckenstaler, Robert; Benndorf, Ralf A.
    • British Journal of Pharmacology, Vol. 177, Issue 7
    • DOI: 10.1111/bph.14991

    Biophysical Approaches Facilitate Computational Drug Discovery for ATP-Binding Cassette Proteins
    journal, January 2017

    • Molinski, Steven V.; Bozóky, Zoltán; Iram, Surtaj H.
    • International Journal of Medicinal Chemistry, Vol. 2017
    • DOI: 10.1155/2017/1529402

    Jump into a New Fold—A Homology Based Model for the ABCG2/BCRP Multidrug Transporter
    journal, October 2016

    Crystal structure of tripartite-type ABC transporter MacB from Acinetobacter baumannii.
    journalarticle, January 2017

    • Okada, Ui; Yamashita, Eiki; Neuberger, Arthur
    • Springer Science and Business Media LLC
    • DOI: 10.17863/cam.17993

    X-ray diffraction reveals the intrinsic difference in the physical properties of membrane and soluble proteins.
    journalarticle, January 2017

    • Robert, Xavier; Kassis-Sahyoun, Josiane; Ceres, Nicoletta
    • Springer Science and Business Media LLC
    • DOI: 10.17863/cam.22258

    Antibiotic Resistance Mediated by the MacB ABC Transporter Family: A Structural and Functional Perspective.
    text, January 2018

    • Greene, Nicholas; Kaplan, Elise; Crow, Allister
    • Apollo - University of Cambridge Repository
    • DOI: 10.17863/cam.26693

    Relationship between conformation shift and disease related variation sites in ATP-binding cassette transporter proteins
    journal, January 2019

    ABCG2 polymorphisms in gout: insights into disease susceptibility and treatment approaches
    journal, April 2017

    • Cleophas, Maartje; Joosten, Leo; Stamp, Lisa
    • Pharmacogenomics and Personalized Medicine, Vol. Volume 10
    • DOI: 10.2147/pgpm.s105854

    Genes Potentially Associated with Familial Hypercholesterolemia
    journal, November 2019

    • Mikhailova, Svetlana; Ivanoshchuk, Dinara; Timoshchenko, Olga
    • Biomolecules, Vol. 9, Issue 12
    • DOI: 10.3390/biom9120807

    Disruption of the Unique ABCG-Family NBD:NBD Interface Impacts Both Drug Transport and ATP Hydrolysis
    journal, January 2020

    • Kapoor, Parth; Briggs, Deborah A.; Cox, Megan H.
    • International Journal of Molecular Sciences, Vol. 21, Issue 3
    • DOI: 10.3390/ijms21030759

    A Newly Integrated Model for Intestinal Cholesterol Absorption and Efflux Reappraises How Plant Sterol Intake Reduces Circulating Cholesterol Levels
    journal, February 2019

    • Nakano, Takanari; Inoue, Ikuo; Murakoshi, Takayuki
    • Nutrients, Vol. 11, Issue 2
    • DOI: 10.3390/nu11020310
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