Towards repurposing the yeast peroxisome for compartmentalizing heterologous metabolic pathways
Abstract
Compartmentalization of enzymes into organelles is a promising strategy for limiting metabolic crosstalk and improving pathway efficiency, but improved tools and design rules are needed to make this strategy available to more engineered pathways. Here we focus on the Saccharomyces cerevisiae peroxisome and develop a sensitive high-throughput assay for peroxisomal cargo import. We identify an enhanced peroxisomal targeting signal type 1 (PTS1) for rapidly sequestering non-native cargo proteins. Additionally, we perform the first systematic in vivo measurements of nonspecific metabolite permeability across the peroxisomal membrane using a polymer exclusion assay. Finally, we apply these new insights to compartmentalize a two-enzyme pathway in the peroxisome and characterize the expression regimes where compartmentalization leads to improved product titre. Lastly, this work builds a foundation for using the peroxisome as a synthetic organelle, highlighting both promise and future challenges on the way to realizing this goal.
- Publication Date:
- Research Org.:
- University of California, Berkeley, CA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC)
- OSTI Identifier:
- 1255249
- Grant/Contract Number:
- SC0008084
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Nature Communications
- Additional Journal Information:
- Journal Volume: 7; Journal ID: ISSN 2041-1723
- Publisher:
- Nature Publishing Group
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
DeLoache, William C., Russ, Zachary N., and Dueber, John E. Towards repurposing the yeast peroxisome for compartmentalizing heterologous metabolic pathways. United States: N. p., 2016.
Web. doi:10.1038/ncomms11152.
DeLoache, William C., Russ, Zachary N., & Dueber, John E. Towards repurposing the yeast peroxisome for compartmentalizing heterologous metabolic pathways. United States. https://doi.org/10.1038/ncomms11152
DeLoache, William C., Russ, Zachary N., and Dueber, John E. Wed .
"Towards repurposing the yeast peroxisome for compartmentalizing heterologous metabolic pathways". United States. https://doi.org/10.1038/ncomms11152. https://www.osti.gov/servlets/purl/1255249.
@article{osti_1255249,
title = {Towards repurposing the yeast peroxisome for compartmentalizing heterologous metabolic pathways},
author = {DeLoache, William C. and Russ, Zachary N. and Dueber, John E.},
abstractNote = {Compartmentalization of enzymes into organelles is a promising strategy for limiting metabolic crosstalk and improving pathway efficiency, but improved tools and design rules are needed to make this strategy available to more engineered pathways. Here we focus on the Saccharomyces cerevisiae peroxisome and develop a sensitive high-throughput assay for peroxisomal cargo import. We identify an enhanced peroxisomal targeting signal type 1 (PTS1) for rapidly sequestering non-native cargo proteins. Additionally, we perform the first systematic in vivo measurements of nonspecific metabolite permeability across the peroxisomal membrane using a polymer exclusion assay. Finally, we apply these new insights to compartmentalize a two-enzyme pathway in the peroxisome and characterize the expression regimes where compartmentalization leads to improved product titre. Lastly, this work builds a foundation for using the peroxisome as a synthetic organelle, highlighting both promise and future challenges on the way to realizing this goal.},
doi = {10.1038/ncomms11152},
journal = {Nature Communications},
number = ,
volume = 7,
place = {United States},
year = {Wed Mar 30 00:00:00 EDT 2016},
month = {Wed Mar 30 00:00:00 EDT 2016}
}
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