Contributions of immunoaffinity chromatography to deep proteome profiling of human biofluids
Abstract
Human biofluids, especially blood plasma or serum, hold great potential as the sources of candidate biomarkers for various diseases; however, the enormous dynamic range of protein concentrations in biofluids represents a significant analytical challenge for detecting promising low-abundance proteins. Over the last decade, various immunoaffinity chromatographic methods have been developed and routinely applied for separating low-abundance proteins from the high- and moderate-abundance proteins, thus enabling much more effective detection of low-abundance proteins. Herein, we review the advances of immunoaffinity separation methods and their contributions to the proteomic applications in human biofluids. The limitations and future perspectives of immunoaffinity separation methods are also discussed.
- Publication Date:
- Research Org.:
- Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1251560
- Report Number(s):
- PNNL-SA-112289
Journal ID: ISSN 1570-0232; PII: S1570023216300150
- Grant/Contract Number:
- AC05-76RL01830
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Journal of Chromatography. B
- Additional Journal Information:
- Journal Volume: 1021; Journal Issue: C; Journal ID: ISSN 1570-0232
- Publisher:
- Elsevier
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; biofluid; immunoaffinity chromatography; plasma/serum; immunodepletion; immunoenrichment; biomarker discovery; proteomics; affinity proteomics
Citation Formats
Wu, Chaochao, Duan, Jicheng, Liu, Tao, Smith, Richard D., and Qian, Wei -Jun. Contributions of immunoaffinity chromatography to deep proteome profiling of human biofluids. United States: N. p., 2016.
Web. doi:10.1016/j.jchromb.2016.01.015.
Wu, Chaochao, Duan, Jicheng, Liu, Tao, Smith, Richard D., & Qian, Wei -Jun. Contributions of immunoaffinity chromatography to deep proteome profiling of human biofluids. United States. https://doi.org/10.1016/j.jchromb.2016.01.015
Wu, Chaochao, Duan, Jicheng, Liu, Tao, Smith, Richard D., and Qian, Wei -Jun. Tue .
"Contributions of immunoaffinity chromatography to deep proteome profiling of human biofluids". United States. https://doi.org/10.1016/j.jchromb.2016.01.015. https://www.osti.gov/servlets/purl/1251560.
@article{osti_1251560,
title = {Contributions of immunoaffinity chromatography to deep proteome profiling of human biofluids},
author = {Wu, Chaochao and Duan, Jicheng and Liu, Tao and Smith, Richard D. and Qian, Wei -Jun},
abstractNote = {Human biofluids, especially blood plasma or serum, hold great potential as the sources of candidate biomarkers for various diseases; however, the enormous dynamic range of protein concentrations in biofluids represents a significant analytical challenge for detecting promising low-abundance proteins. Over the last decade, various immunoaffinity chromatographic methods have been developed and routinely applied for separating low-abundance proteins from the high- and moderate-abundance proteins, thus enabling much more effective detection of low-abundance proteins. Herein, we review the advances of immunoaffinity separation methods and their contributions to the proteomic applications in human biofluids. The limitations and future perspectives of immunoaffinity separation methods are also discussed.},
doi = {10.1016/j.jchromb.2016.01.015},
journal = {Journal of Chromatography. B},
number = C,
volume = 1021,
place = {United States},
year = {Tue Jan 12 00:00:00 EST 2016},
month = {Tue Jan 12 00:00:00 EST 2016}
}
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