Matrix assisted laser desorption/ionization-mass spectrometry imaging (MALDI-MSI) for direct visualization of plant metabolites in situ
Abstract
Direct visualization of plant tissues by matrix assisted laser desorption ionization-mass spectrometry imaging (MALDI-MSI) has revealed key insights into the localization of metabolites in situ. Recent efforts have determined the spatial distribution of primary and secondary metabolites in plant tissues and cells. Strategies have been applied in many areas of metabolism including isotope flux analyses, plant interactions, and transcriptional regulation of metabolite accumulation. Technological advances have pushed achievable spatial resolution to subcellular levels and increased instrument sensitivity by several orders of magnitude. Furthermore, it is anticipated that MALDI-MSI and other MSI approaches will bring a new level of understanding to metabolomics as scientists will be encouraged to consider spatial heterogeneity of metabolites in descriptions of metabolic pathway regulation.
- Authors:
-
- Univ. of North Texas, Denton, TX (United States)
- Iowa State Univ., Ames, IA (United States); Ames Lab., Ames, IA (United States)
- Publication Date:
- Research Org.:
- Ames Laboratory (AMES), Ames, IA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1249347
- Report Number(s):
- IS-J-8967
Journal ID: ISSN 0958-1669; PII: S0958166915001421
- Grant/Contract Number:
- FG02-14ER15647; AC02-07CH11358
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Current Opinion in Biotechnology
- Additional Journal Information:
- Journal Volume: 37; Journal Issue: C; Journal ID: ISSN 0958-1669
- Publisher:
- Elsevier
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 47 OTHER INSTRUMENTATION; 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Sturtevant, Drew, Lee, Young -Jin, and Chapman, Kent D. Matrix assisted laser desorption/ionization-mass spectrometry imaging (MALDI-MSI) for direct visualization of plant metabolites in situ. United States: N. p., 2015.
Web. doi:10.1016/j.copbio.2015.10.004.
Sturtevant, Drew, Lee, Young -Jin, & Chapman, Kent D. Matrix assisted laser desorption/ionization-mass spectrometry imaging (MALDI-MSI) for direct visualization of plant metabolites in situ. United States. https://doi.org/10.1016/j.copbio.2015.10.004
Sturtevant, Drew, Lee, Young -Jin, and Chapman, Kent D. Sun .
"Matrix assisted laser desorption/ionization-mass spectrometry imaging (MALDI-MSI) for direct visualization of plant metabolites in situ". United States. https://doi.org/10.1016/j.copbio.2015.10.004. https://www.osti.gov/servlets/purl/1249347.
@article{osti_1249347,
title = {Matrix assisted laser desorption/ionization-mass spectrometry imaging (MALDI-MSI) for direct visualization of plant metabolites in situ},
author = {Sturtevant, Drew and Lee, Young -Jin and Chapman, Kent D.},
abstractNote = {Direct visualization of plant tissues by matrix assisted laser desorption ionization-mass spectrometry imaging (MALDI-MSI) has revealed key insights into the localization of metabolites in situ. Recent efforts have determined the spatial distribution of primary and secondary metabolites in plant tissues and cells. Strategies have been applied in many areas of metabolism including isotope flux analyses, plant interactions, and transcriptional regulation of metabolite accumulation. Technological advances have pushed achievable spatial resolution to subcellular levels and increased instrument sensitivity by several orders of magnitude. Furthermore, it is anticipated that MALDI-MSI and other MSI approaches will bring a new level of understanding to metabolomics as scientists will be encouraged to consider spatial heterogeneity of metabolites in descriptions of metabolic pathway regulation.},
doi = {10.1016/j.copbio.2015.10.004},
journal = {Current Opinion in Biotechnology},
number = C,
volume = 37,
place = {United States},
year = {Sun Nov 22 00:00:00 EST 2015},
month = {Sun Nov 22 00:00:00 EST 2015}
}
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