Immuno and Affinity Cytochemical Analysis of Cell Wall Composition in the Moss Physcomitrella patens
Abstract
In contrast to homeohydric vascular plants, mosses employ a poikilohydric strategy for surviving in the dry aerial environment. A detailed understanding of the structure, composition, and development of moss cell walls can contribute to our understanding of not only the evolution of overall cell wall complexity, but also the differences that have evolved in response to selection for different survival strategies. The model moss species Physcomitrella patens has a predominantly haploid lifecycle consisting of protonemal filaments that regenerate from protoplasts and enlarge by tip growth, and leafy gametophores composed of cells that enlarge by diffuse growth and differentiate into several different types. Advantages for genetic studies include methods for efficient targeted gene modification and extensive genomic resources. Immuno and affinity cytochemical labeling were used to examine the distribution of polysaccharides and proteins in regenerated protoplasts, protonemal filaments, rhizoids, and sectioned gametophores of P. patens. The cell wall composition of regenerated protoplasts was also characterized by flow cytometry. Crystalline cellulose was abundant in the cell walls of regenerating protoplasts and protonemal cells that developed on media of high osmolarity, whereas homogalactuonan was detected in the walls of protonemal cells that developed on low osmolarity media and not in regenerating protoplasts. Mannanmore »
- Authors:
-
- Univ. of Rhode Island, Kingston, RI (United States). Dept. of Biological Sciences
- Publication Date:
- Research Org.:
- Univ. of Rhode Island, Kingston, RI (United States). Dept. of Biological Sciences; Energy Frontier Research Centers (EFRC) (United States). Center for Lignocellulose Structure and Formation (CLSF)
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES)
- OSTI Identifier:
- 1249327
- Grant/Contract Number:
- SC0001090; IOS-1257047
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Frontiers in Plant Science
- Additional Journal Information:
- Journal Volume: 7; Journal ID: ISSN 1664-462X
- Publisher:
- Frontiers Research Foundation
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 60 APPLIED LIFE SCIENCES; affinity cytochemistry; bryophyte; cell wall, flow cytometry; immunofluorescence; Physcomitrella patens
Citation Formats
Berry, Elizabeth A., Tran, Mai L., Dimos, Christos S., Budziszek, Michael J., Scavuzzo-Duggan, Tess R., and Roberts, Alison W. Immuno and Affinity Cytochemical Analysis of Cell Wall Composition in the Moss Physcomitrella patens. United States: N. p., 2016.
Web. doi:10.3389/fpls.2016.00248.
Berry, Elizabeth A., Tran, Mai L., Dimos, Christos S., Budziszek, Michael J., Scavuzzo-Duggan, Tess R., & Roberts, Alison W. Immuno and Affinity Cytochemical Analysis of Cell Wall Composition in the Moss Physcomitrella patens. United States. https://doi.org/10.3389/fpls.2016.00248
Berry, Elizabeth A., Tran, Mai L., Dimos, Christos S., Budziszek, Michael J., Scavuzzo-Duggan, Tess R., and Roberts, Alison W. Tue .
"Immuno and Affinity Cytochemical Analysis of Cell Wall Composition in the Moss Physcomitrella patens". United States. https://doi.org/10.3389/fpls.2016.00248. https://www.osti.gov/servlets/purl/1249327.
@article{osti_1249327,
title = {Immuno and Affinity Cytochemical Analysis of Cell Wall Composition in the Moss Physcomitrella patens},
author = {Berry, Elizabeth A. and Tran, Mai L. and Dimos, Christos S. and Budziszek, Michael J. and Scavuzzo-Duggan, Tess R. and Roberts, Alison W.},
abstractNote = {In contrast to homeohydric vascular plants, mosses employ a poikilohydric strategy for surviving in the dry aerial environment. A detailed understanding of the structure, composition, and development of moss cell walls can contribute to our understanding of not only the evolution of overall cell wall complexity, but also the differences that have evolved in response to selection for different survival strategies. The model moss species Physcomitrella patens has a predominantly haploid lifecycle consisting of protonemal filaments that regenerate from protoplasts and enlarge by tip growth, and leafy gametophores composed of cells that enlarge by diffuse growth and differentiate into several different types. Advantages for genetic studies include methods for efficient targeted gene modification and extensive genomic resources. Immuno and affinity cytochemical labeling were used to examine the distribution of polysaccharides and proteins in regenerated protoplasts, protonemal filaments, rhizoids, and sectioned gametophores of P. patens. The cell wall composition of regenerated protoplasts was also characterized by flow cytometry. Crystalline cellulose was abundant in the cell walls of regenerating protoplasts and protonemal cells that developed on media of high osmolarity, whereas homogalactuonan was detected in the walls of protonemal cells that developed on low osmolarity media and not in regenerating protoplasts. Mannan was the major hemicellulose detected in all tissues tested. Arabinogalactan proteins were detected in different cell types by different probes, consistent with structural heterogneity. The results reveal developmental and cell type specific differences in cell wall composition and provide a basis for analyzing cell wall phenotypes in knockout mutants.},
doi = {10.3389/fpls.2016.00248},
journal = {Frontiers in Plant Science},
number = ,
volume = 7,
place = {United States},
year = {Tue Mar 08 00:00:00 EST 2016},
month = {Tue Mar 08 00:00:00 EST 2016}
}
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Microarray analysis of the moss Physcomitrella patens reveals evolutionarily conserved transcriptional regulation of salt stress and abscisic acid signalling
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An extended set of monoclonal antibodies to pectic homogalacturonan
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Evolution and diversity of green plant cell walls
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Diversity in the distribution of polysaccharide and glycoprotein epitopes in the cell walls of bryophytes: new evidence for the multiple evolution of water-conducting cells
journal, December 2002
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Comparative genomics of Physcomitrella patens gametophytic transcriptome and Arabidopsis thaliana: Implication for land plant evolution
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Physcomitrella patens : A moss system for the study of plant cell walls
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Cytokinesis in tobacco BY-2 and root tip cells: a new model of cell plate formation in higher plants
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Novel localization of callose in the spores of Physcomitrella patens and phylogenomics of the callose synthase gene family
journal, January 2009
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- Annals of Botany, Vol. 103, Issue 5
Moss and liverwort xyloglucans contain galacturonic acid and are structurally distinct from the xyloglucans synthesized by hornworts and vascular plants*
journal, August 2008
- Peña, Maria J.; Darvill, Alan G.; Eberhard, Stefan
- Glycobiology, Vol. 18, Issue 11
The ability of land plants to synthesize glucuronoxylans predates the evolution of tracheophytes
journal, November 2011
- Kulkarni, Ameya R.; Peña, Maria J.; Avci, Utku
- Glycobiology, Vol. 22, Issue 3
Both chloronemal and caulonemal cells expand by tip growth in the moss Physcomitrella patens
journal, April 2007
- Menand, Benoît; Calder, Grant; Dolan, Liam
- Journal of Experimental Botany, Vol. 58, Issue 7
The impact of abiotic factors on cellulose synthesis
journal, November 2015
- Wang, Ting; McFarlane, Heather E.; Persson, Staffan
- Journal of Experimental Botany, Vol. 67, Issue 2
Cell Wall Biology: Perspectives from Cell Wall Imaging
journal, March 2011
- Lee, Kieran J. D.; Marcus, Susan E.; Knox, J. Paul
- Molecular Plant, Vol. 4, Issue 2
Functional Genomic Analysis Supports Conservation of Function Among Cellulose Synthase-Like A Gene Family Members and Suggests Diverse Roles of Mannans in Plants
journal, February 2007
- Liepman, Aaron H.; Nairn, C. Joseph; Willats, William G. T.
- Plant Physiology, Vol. 143, Issue 4
Developmental Stage Specificity and the Role of Mitochondrial Metabolism in the Response of Arabidopsis Leaves to Prolonged Mild Osmotic Stress
journal, November 2009
- Skirycz, Aleksandra; De Bodt, Stefanie; Obata, Toshihiro
- Plant Physiology, Vol. 152, Issue 1
A Comprehensive Toolkit of Plant Cell Wall Glycan-Directed Monoclonal Antibodies
journal, April 2010
- Pattathil, S.; Avci, U.; Baldwin, D.
- Plant Physiology, Vol. 153, Issue 2, p. 514-525
Evolution and Function of the Plant Cell Wall Synthesis-Related Glycosyltransferase Family 8
journal, June 2010
- Yin, Yanbin; Chen, Huiling; Hahn, Michael G.
- Plant Physiology, Vol. 153, Issue 4
Localization of Pectic Galactan in Tomato Cell Walls Using a Monoclonal Antibody Specific to (1[->]4)-[beta]-D-Galactan
journal, April 1997
- Jones, L.; Seymour, G. B.; Knox, J. P.
- Plant Physiology, Vol. 113, Issue 4
Alteration of the Physical and Chemical Structure of the Primary Cell Wall of Growth-Limited Plant Cells Adapted to Osmotic Stress
journal, September 1989
- Iraki, Naim M.; Bressan, Ray A.; Hasegawa, P. M.
- Plant Physiology, Vol. 91, Issue 1
Cell Walls of Tobacco Cells and Changes in Composition Associated with Reduced Growth upon Adaptation to Water and Saline Stress
journal, September 1989
- Iraki, Naim M.; Singh, Narendra; Bressan, Ray A.
- Plant Physiology, Vol. 91, Issue 1
Arabinogalactan Proteins Are Required for Apical Cell Extension in the Moss Physcomitrella patens
journal, September 2005
- Lee, Kieran J. D.; Sakata, Yoichi; Mau, Shaio-Lim
- The Plant Cell, Vol. 17, Issue 11
Flow sorting and culture of plant protoplasts
journal, January 1984
- Harkins, Kristi R.; Galbraith, David W.
- Physiologia Plantarum, Vol. 60, Issue 1
Microarray analysis of transcriptional responses to abscisic acid and osmotic, salt, and drought stress in the moss, Physcomitrella patens
journal, October 2007
- Cuming, Andrew C.; Cho, Sung Hyun; Kamisugi, Yasuko
- New Phytologist, Vol. 176, Issue 2
Mapping the walls of the kingdom: the view from the horsetails
journal, July 2008
- Knox, J. Paul
- New Phytologist, Vol. 179, Issue 1
Arabidopsis thaliana IRX10 and two related proteins from psyllium and Physcomitrella patens are xylan xylosyltransferases
journal, September 2014
- Jensen, Jacob Krüger; Johnson, Nathan Robert; Wilkerson, Curtis Gene
- The Plant Journal, Vol. 80, Issue 2
The Physcomitrella Genome Reveals Evolutionary Insights into the Conquest of Land by Plants
journal, December 2007
- Rensing, S. A.; Lang, D.; Zimmer, A. D.
- Science, Vol. 319, Issue 5859
Callose deposition in the primary wall of suspension cells and regenerating protoplasts, and its relationship to patterned cellulose synthesis
journal, July 1996
- Amstel, Ton N. M. van; Kengen, Harry M. P.
- Canadian Journal of Botany, Vol. 74, Issue 7
Partial functional conservation of IRX10 homologs in physcomitrella patens and Arabidopsis thaliana indicates an evolutionary step contributing to vascular formation in land plants
journal, January 2013
- Hörnblad, Emma; Ulfstedt, Mikael; Ronne, Hans
- BMC Plant Biology, Vol. 13, Issue 1
Phylogenetic analysis of pectin-related gene families in Physcomitrella patens and nine other plant species yields evolutionary insights into cell walls
journal, January 2014
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Works referencing / citing this record:
Moss Pathogenesis-Related-10 Protein Enhances Resistance to Pythium irregulare in Physcomitrella patens and Arabidopsis thaliana
journal, April 2016
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