Understanding dynamic changes in live cell adhesion with neutron reflectometry
Abstract
In this study, neutron reflectometry (NR) was used to examine various live cells' adhesion to quartz substrates under different environmental conditions, including flow stress. To the best of our knowledge, these measurements represent the first successful visualization and quantization of the interface between live cells and a substrate with sub-nanometer resolution. In our first experiments, we examined live mouse fibroblast cells as opposed to past experiments using supported lipids, proteins, or peptide layers with no associated cells. We continued the NR studies of cell adhesion by investigating endothelial monolayers and glioblastoma cells under dynamic flow conditions. We demonstrated that neutron reflectometry is a powerful tool to study the strength of cellular layer adhesion in living tissues, which is a key factor in understanding the physiology of cell interactions and conditions leading to abnormal or disease circumstances. Continuative measurements, such as investigating changes in tumor cell — surface contact of various glioblastomas, could impact advancements in tumor treatments. In principle, this can help us to identify changes that correlate with tumor invasiveness. Pursuit of these studies can have significant medical impact on the understanding of complex biological problems and their effective treatment, e.g. for the development of targeted anti-invasive therapies.
- Authors:
-
- Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
- California Institute of Technology, Pasadena, CA (United States)
- Univ. of Pittsburgh Medical Center, Pittsburgh, PA (United States)
- NDA Analytics, Cambridgeshire (United Kingdom)
- Univ. of Chicago, Chicago, IL (United States)
- Brigham and Women's Hospital and Harvard Medical School, Boston, MA (United States)
- Publication Date:
- Research Org.:
- Los Alamos National Laboratory (LANL), Los Alamos, NM (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1246927
- Report Number(s):
- LA-UR-14-26647
Journal ID: ISSN 0217-9849
- Grant/Contract Number:
- AC52-06NA25396
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Modern Physics Letters B
- Additional Journal Information:
- Journal Volume: 28; Journal Issue: 30; Journal ID: ISSN 0217-9849
- Publisher:
- World Scientific Publishing
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; cells; endothelial monolayer; glioblastomas; adhesion; shear stress; neutron scattering
Citation Formats
Junghans, Ann, Waltman, Mary Jo, Smith, Hillary L., Pocivavsek, Luka, Zebda, Noureddine, Birukov, Konstantin, Viapiano, Mariano, and Majewski, Jaroslaw. Understanding dynamic changes in live cell adhesion with neutron reflectometry. United States: N. p., 2014.
Web. doi:10.1142/S0217984914300154.
Junghans, Ann, Waltman, Mary Jo, Smith, Hillary L., Pocivavsek, Luka, Zebda, Noureddine, Birukov, Konstantin, Viapiano, Mariano, & Majewski, Jaroslaw. Understanding dynamic changes in live cell adhesion with neutron reflectometry. United States. https://doi.org/10.1142/S0217984914300154
Junghans, Ann, Waltman, Mary Jo, Smith, Hillary L., Pocivavsek, Luka, Zebda, Noureddine, Birukov, Konstantin, Viapiano, Mariano, and Majewski, Jaroslaw. Wed .
"Understanding dynamic changes in live cell adhesion with neutron reflectometry". United States. https://doi.org/10.1142/S0217984914300154. https://www.osti.gov/servlets/purl/1246927.
@article{osti_1246927,
title = {Understanding dynamic changes in live cell adhesion with neutron reflectometry},
author = {Junghans, Ann and Waltman, Mary Jo and Smith, Hillary L. and Pocivavsek, Luka and Zebda, Noureddine and Birukov, Konstantin and Viapiano, Mariano and Majewski, Jaroslaw},
abstractNote = {In this study, neutron reflectometry (NR) was used to examine various live cells' adhesion to quartz substrates under different environmental conditions, including flow stress. To the best of our knowledge, these measurements represent the first successful visualization and quantization of the interface between live cells and a substrate with sub-nanometer resolution. In our first experiments, we examined live mouse fibroblast cells as opposed to past experiments using supported lipids, proteins, or peptide layers with no associated cells. We continued the NR studies of cell adhesion by investigating endothelial monolayers and glioblastoma cells under dynamic flow conditions. We demonstrated that neutron reflectometry is a powerful tool to study the strength of cellular layer adhesion in living tissues, which is a key factor in understanding the physiology of cell interactions and conditions leading to abnormal or disease circumstances. Continuative measurements, such as investigating changes in tumor cell — surface contact of various glioblastomas, could impact advancements in tumor treatments. In principle, this can help us to identify changes that correlate with tumor invasiveness. Pursuit of these studies can have significant medical impact on the understanding of complex biological problems and their effective treatment, e.g. for the development of targeted anti-invasive therapies.},
doi = {10.1142/S0217984914300154},
journal = {Modern Physics Letters B},
number = 30,
volume = 28,
place = {United States},
year = {Wed Dec 10 00:00:00 EST 2014},
month = {Wed Dec 10 00:00:00 EST 2014}
}
Web of Science
Works referenced in this record:
Signal transduction pathways activated in human pulmonary endothelial cells by OxPAPC, a bioactive component of oxidized lipoproteins
journal, January 2004
- Birukov, Konstantin G.; Leitinger, Norbert; Bochkov, Valery N.
- Microvascular Research, Vol. 67, Issue 1
Elementary Mechanics of the Endothelium of Blood Vessels
journal, February 1993
- Fung, Y. C.; Liu, S. Q.
- Journal of Biomechanical Engineering, Vol. 115, Issue 1
The Mechanism of Adhesion of Cells to Glass
journal, February 1964
- Curtis, A. S. G.
- The Journal of Cell Biology, Vol. 20, Issue 2
Fluorescence interference-contrast microscopy of cell adhesion on oxidized silicon
journal, October 1997
- Braun, D.; Fromherz, P.
- Applied Physics A: Materials Science & Processing, Vol. 65, Issue 4-5
Cell-substrate contacts illuminated by total internal reflection fluorescence.
journal, April 1981
- Axelrod, D.
- The Journal of Cell Biology, Vol. 89, Issue 1
Quantitative analysis of variable-angle total internal reflection fluorescence microscopy (VA-TIRFM) of cell/substrate contacts
journal, January 1994
- Burmeister, J. S.; Truskey, G. A.; Reichert, W. M.
- Journal of Microscopy, Vol. 173, Issue 1
Topography of cell-glass apposition revealed by total internal reflection fluorescence of volume markers.
journal, April 1985
- Gingell, D.; Todd, I.; Bailey, J.
- The Journal of Cell Biology, Vol. 100, Issue 4
Total Internal Reflection Fluorescence Microscopy of Cell Adhesion on Patterned Self-Assembled Monolayers on Gold
journal, March 2009
- Hoover, Diana K.; Lee, Eun-Ju; Yousaf, Muhammad N.
- Langmuir, Vol. 25, Issue 5
Surface plasmon resonance imaging of cells and surface-associated fibronectin
journal, February 2009
- Peterson, Alexander W.; Halter, Michael; Tona, Alessandro
- BMC Cell Biology, Vol. 10, Issue 1
Neutron reflectivity at the solid/liquid interface: examples of applications in biophysics
journal, May 2001
- Fragneto-Cusani, Giovanna
- Journal of Physics: Condensed Matter, Vol. 13, Issue 21
Neutron reflection from interfaces with biological and biomimetic materials
journal, May 2001
- Krueger, Susan
- Current Opinion in Colloid & Interface Science, Vol. 6, Issue 2
Structure of Phospholipid Monolayers Containing Poly(ethylene glycol) Lipids at the Air−Water Interface
journal, April 1997
- Majewski, J.; Kuhl, T. L.; Gerstenberg, M. C.
- The Journal of Physical Chemistry B, Vol. 101, Issue 16
Electric Field-Driven Transformations of a Supported Model Biological Membrane—An Electrochemical and Neutron Reflectivity Study
journal, March 2004
- Burgess, I.; Li, M.; Horswell, S. L.
- Biophysical Journal, Vol. 86, Issue 3
Interaction of the Third Helix of Antennapedia Homeodomain with a Deposited Phospholipid Bilayer: A Neutron Reflectivity Structural Study
journal, May 2000
- Fragneto, G.; Graner, F.; Charitat, T.
- Langmuir, Vol. 16, Issue 10
Neutron Reflectivity and Atomic Force Microscopy Studies of a Lipid Bilayer in Water Adsorbed to the Surface of a Silicon Single Crystal
journal, January 1996
- Koenig, B. W.; Krueger, S.; Orts, W. J.
- Langmuir, Vol. 12, Issue 5
Extending the Angular Range of Neutron Reflectivity Measurements from Planar Lipid Bilayers: Application to a Model Biological Membrane
journal, August 1995
- Krueger, S.; Ankner, J. F.; Satija, S. K.
- Langmuir, Vol. 11, Issue 8
Neutron Reflectivity Study of Lipid Membranes Assembled on Ordered Nanocomposite and Nanoporous Silica Thin Films
journal, March 2005
- Doshi, Dhaval A.; Dattelbaum, Andrew M.; Watkins, Erik B.
- Langmuir, Vol. 21, Issue 7, p. 2865-2870
SPEAR — ToF neutron reflectometer at the Los Alamos Neutron Science Center
journal, November 2011
- Dubey, M.; Jablin, M. S.; Wang, P.
- The European Physical Journal Plus, Vol. 126, Issue 11
Synchrotron x-ray studies of liquid-vapor interfaces
journal, December 1986
- Als-Nielsen, J.
- Physica A: Statistical Mechanics and its Applications, Vol. 140, Issue 1-2
The application of the specular reflection of neutrons to the study of surfaces and interfaces
journal, February 1990
- Penfold, J.; Thomas, R. K.
- Journal of Physics: Condensed Matter, Vol. 2, Issue 6
Co-refinement of multiple-contrast neutron/X-ray reflectivity data using MOTOFIT
journal, March 2006
- Nelson, Andrew
- Journal of Applied Crystallography, Vol. 39, Issue 2
Mouse Fibroblast Cell Adhesion Studied by Neutron Reflectometry
journal, March 2010
- Smith, Hillary L.; Hickey, Joseph; Jablin, Michael S.
- Biophysical Journal, Vol. 98, Issue 5
Tuning endothelial monolayer adhesion: a neutron reflectivity study
journal, January 2014
- Pocivavsek, Luka; Junghans, Ann; Zebda, Noureddine
- American Journal of Physiology-Lung Cellular and Molecular Physiology, Vol. 306, Issue 1
Shear Stress-Mediated Cytoskeletal Remodeling and Cortactin Translocation in Pulmonary Endothelial Cells
journal, April 2002
- Birukov, Konstantin G.; Birukova, Anna A.; Dudek, Steven M.
- American Journal of Respiratory Cell and Molecular Biology, Vol. 26, Issue 4
Differential Regulation of Pulmonary Endothelial Monolayer Integrity by Varying Degrees of Cyclic Stretch
journal, May 2006
- Birukova, Anna A.; Chatchavalvanich, Santipongse; Rios, Alexander
- The American Journal of Pathology, Vol. 168, Issue 5
Atheroma and arterial wall shear - Observation, correlation and proposal of a shear dependent mass transfer mechanism for atherogenesis
journal, February 1971
- Caro, C. G.; Fitz-Gerald, J. M.; Schroter, R. C.
- Proceedings of the Royal Society of London. Series B. Biological Sciences, Vol. 177, Issue 1046, p. 109-133
The shear stress of it all: the cell membrane and mechanochemical transduction
journal, June 2007
- White, Charles R.; Frangos, John A.
- Philosophical Transactions of the Royal Society B: Biological Sciences, Vol. 362, Issue 1484
Characterization of the collagen synthesized by endothelial cells in culture.
journal, July 1976
- Howard, B. V.; Macarak, E. J.; Gunson, D.
- Proceedings of the National Academy of Sciences, Vol. 73, Issue 7
Mammalian collagen IV
journal, January 2008
- Khoshnoodi, Jamshid; Pedchenko, Vadim; Hudson, Billy G.
- Microscopy Research and Technique, Vol. 71, Issue 5
Characterization of Endothelial Basement Membrane Nanotopography in Rhesus Macaque as a Guide for Vessel Tissue Engineering
journal, September 2009
- Liliensiek, Sara J.; Nealey, Paul; Murphy, Christopher J.
- Tissue Engineering Part A, Vol. 15, Issue 9
Nanoparticles in Block-Copolymer Films Studied by Specular and Off-Specular Neutron Scattering †
journal, September 2003
- Lauter-Pasyuk, V.; Lauter, H. J.; Gordeev, G. P.
- Langmuir, Vol. 19, Issue 19
In-Plane Correlations in a Polymer-Supported Lipid Membrane Measured by Off-Specular Neutron Scattering
journal, March 2011
- Jablin, Michael S.; Zhernenkov, Mikhail; Toperverg, Boris P.
- Physical Review Letters, Vol. 106, Issue 13
Tumor Endothelial Cells
journal, October 2011
- Dudley, A. C.
- Cold Spring Harbor Perspectives in Medicine, Vol. 2, Issue 3
A morphological biosensor for mammalian cells
journal, December 1993
- Giaever, Ivar; Keese, Charles R.
- Nature, Vol. 366, Issue 6455
Electrical method for detection of endothelial cell shape change in real time: assessment of endothelial barrier function.
journal, September 1992
- Tiruppathi, C.; Malik, A. B.; Del Vecchio, P. J.
- Proceedings of the National Academy of Sciences, Vol. 89, Issue 17
Molecular Pathology of Malignant Gliomas
journal, February 2006
- Louis, David N.
- Annual Review of Pathology: Mechanisms of Disease, Vol. 1, Issue 1
The cellular origin for malignant glioma and prospects for clinical advancements
journal, May 2012
- Zong, Hui; Verhaak, Roel GW; Canoll, Peter
- Expert Review of Molecular Diagnostics, Vol. 12, Issue 4
Glioblastoma and Other Malignant Gliomas: A Clinical Review
journal, November 2013
- Omuro, Antonio
- JAMA, Vol. 310, Issue 17
Anti-VEGF therapies for malignant glioma: treatment effects and escape mechanisms
journal, March 2009
- Miletic, Hrvoje; Niclou, Simone P.; Johansson, Mikael
- Expert Opinion on Therapeutic Targets, Vol. 13, Issue 4
Molecular targets of glioma invasion
journal, January 2007
- Nakada, M.; Nakada, S.; Demuth, T.
- Cellular and Molecular Life Sciences, Vol. 64, Issue 4
Interstitial guidance of cancer invasion
journal, December 2011
- G. Gritsenko, Pavlo; Ilina, Olga; Friedl, Peter
- The Journal of Pathology, Vol. 226, Issue 2
A neurocentric perspective on glioma invasion
journal, June 2014
- Cuddapah, Vishnu Anand; Robel, Stefanie; Watkins, Stacey
- Nature Reviews Neuroscience, Vol. 15, Issue 7
Microregional extracellular matrix heterogeneity in brain modulates glioma cell invasion
journal, June 2004
- Bellail, Anita C.; Hunter, Stephen B.; Brat, Daniel J.
- The International Journal of Biochemistry & Cell Biology, Vol. 36, Issue 6
Glioma Invasion: Mechanisms and Therapeutic Challenges
book, January 2009
- Viapiano, Mariano S.; Lawler, Sean E.
- CNS Cancer
From barriers to bridges: chondroitin sulfate proteoglycans in neuropathology
journal, October 2006
- Viapiano, Mariano S.; Matthews, Russell T.
- Trends in Molecular Medicine, Vol. 12, Issue 10
High interstitial fluid pressure — an obstacle in cancer therapy
journal, October 2004
- Heldin, Carl-Henrik; Rubin, Kristofer; Pietras, Kristian
- Nature Reviews Cancer, Vol. 4, Issue 10
Diffusion parameters of the extracellular space in human gliomas: Diffusion in Human Gliomas
journal, February 2003
- Vargová, Lýdia; Homola, Aleš; Zámečník, Josef
- Glia, Vol. 42, Issue 1
Fluid Shear Stress Regulates the Invasive Potential of Glioma Cells via Modulation of Migratory Activity and Matrix Metalloproteinase Expression
journal, May 2011
- Qazi, Henry; Shi, Zhong-Dong; Tarbell, John M.
- PLoS ONE, Vol. 6, Issue 5
The Murine GL261 Glioma Experimental Model to Assess Novel Brain Tumor Treatments
book, January 2009
- Newcomb, Elizabeth W.; Zagzag, David
- CNS Cancer
A comparative study of glycosaminoglycans in cultures of human, normal and malignant glial cells
journal, March 1979
- Glimelius, B.; Norling, B.; Westermark, B.
- Journal of Cellular Physiology, Vol. 98, Issue 3
Composition and distribution of glycosaminoglycans in cultures of human normal and malignant glial cells
journal, June 1978
- Glimelius, B.; Norling, B.; Westermark, B.
- Biochemical Journal, Vol. 172, Issue 3
Cell Adhesion as Wetting Transition?
book, January 2002
- Sackmann, E.; Bruinsma, R.
- Physics of bio-molecules and cells [Physique des biomolécules et des cellules]
Mechanical Forces on Cells
book, May 2007
- Fisher, John P.; Mikos, Antonios G.; Bronzino, Joseph D.
- Tissue Engineering
Tumor Endothelial Cells
text, January 2012
- C., Dudley, A.
- The University of North Carolina at Chapel Hill University Libraries
Mechanical Forces on Cells
book, April 2016
- Bronzino, Joseph D.
- Tissue Engineering and Artificial Organs
Works referencing / citing this record:
Multi-objective differential evolution based random forest for e-health applications
journal, February 2019
- Kaur, Manjit; Gianey, Hemant Kumar; Singh, Dilbag
- Modern Physics Letters B, Vol. 33, Issue 05