Characterization of the Bacterioferritin/Bacterioferritin Associated Ferredoxin Protein–Protein Interaction in Solution and Determination of Binding Energy Hot Spots
Abstract
Mobilization of iron stored in the interior cavity of BfrB requires electron transfer from the [2Fe–2S] cluster in Bfd to the core iron in BfrB. A crystal structure of the Pseudomonas aeruginosa BfrB:Bfd complex revealed that BfrB can bind up to 12 Bfd molecules at 12 structurally identical binding sites, placing the [2Fe–2S] cluster of each Bfd immediately above a heme group in BfrB. We report here a study aimed at characterizing the strength of the P. aeruginosa BfrB:Bfd association using surface plasmon resonance and isothermal titration calorimetry as well as determining the binding energy hot spots at the protein–protein interaction interface. The results show that the 12 Bfd-binding sites on BfrB are equivalent and independent and that the protein–protein association at each of these sites is driven entropically and is characterized by a dissociation constant (Kd) of approximately 3 μM. Determination of the binding energy hot spots was carried out by replacing certain residues that comprise the protein–protein interface with alanine and by evaluating the effect of the mutation on Kd and on the efficiency of core iron mobilization from BfrB. The results identified hot spot residues in both proteins [LB68, EA81, and EA85 in BfrB (superscript for residuemore »
- Authors:
-
- Univ. of Kansas, Lawrence, KS (United States)
- Hauptman Woodward Medical Research Inst., Argonne, IL (United States). IMCA-CAT
- Publication Date:
- Research Org.:
- Argonne National Laboratory (ANL), Argonne, IL (United States). Advanced Photon Source (APS)
- Sponsoring Org.:
- National Center for Research Resources; National Institute of General Medical Sciences (NIGMS); National Institutes of Health (NIH); National Science Foundation (NSF); Industrial Macromolecular Crystallography Association; USDOE Office of Science (SC); 2014 University of Kansas Strategic Grant
- OSTI Identifier:
- 1240165
- Grant/Contract Number:
- 5P20RR017708-10; 8 P20 GM 103420; AC02-06CH11357; MCB-1158469
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Biochemistry
- Additional Journal Information:
- Journal Volume: 54; Journal Issue: 40; Journal ID: ISSN 0006-2960
- Publisher:
- American Chemical Society (ACS)
- Country of Publication:
- United States
- Language:
- ENGLISH
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; Iron; Bioinorganic chemistry; Genetics; Molecules; Surface plasmon resonance
Citation Formats
Wang, Yan, Yao, Huili, Cheng, Yuan, Lovell, Scott, Battaile, Kevin P., Midaugh, C. Russell, and Rivera, Mario. Characterization of the Bacterioferritin/Bacterioferritin Associated Ferredoxin Protein–Protein Interaction in Solution and Determination of Binding Energy Hot Spots. United States: N. p., 2015.
Web. doi:10.1021/acs.biochem.5b00937.
Wang, Yan, Yao, Huili, Cheng, Yuan, Lovell, Scott, Battaile, Kevin P., Midaugh, C. Russell, & Rivera, Mario. Characterization of the Bacterioferritin/Bacterioferritin Associated Ferredoxin Protein–Protein Interaction in Solution and Determination of Binding Energy Hot Spots. United States. https://doi.org/10.1021/acs.biochem.5b00937
Wang, Yan, Yao, Huili, Cheng, Yuan, Lovell, Scott, Battaile, Kevin P., Midaugh, C. Russell, and Rivera, Mario. Fri .
"Characterization of the Bacterioferritin/Bacterioferritin Associated Ferredoxin Protein–Protein Interaction in Solution and Determination of Binding Energy Hot Spots". United States. https://doi.org/10.1021/acs.biochem.5b00937. https://www.osti.gov/servlets/purl/1240165.
@article{osti_1240165,
title = {Characterization of the Bacterioferritin/Bacterioferritin Associated Ferredoxin Protein–Protein Interaction in Solution and Determination of Binding Energy Hot Spots},
author = {Wang, Yan and Yao, Huili and Cheng, Yuan and Lovell, Scott and Battaile, Kevin P. and Midaugh, C. Russell and Rivera, Mario},
abstractNote = {Mobilization of iron stored in the interior cavity of BfrB requires electron transfer from the [2Fe–2S] cluster in Bfd to the core iron in BfrB. A crystal structure of the Pseudomonas aeruginosa BfrB:Bfd complex revealed that BfrB can bind up to 12 Bfd molecules at 12 structurally identical binding sites, placing the [2Fe–2S] cluster of each Bfd immediately above a heme group in BfrB. We report here a study aimed at characterizing the strength of the P. aeruginosa BfrB:Bfd association using surface plasmon resonance and isothermal titration calorimetry as well as determining the binding energy hot spots at the protein–protein interaction interface. The results show that the 12 Bfd-binding sites on BfrB are equivalent and independent and that the protein–protein association at each of these sites is driven entropically and is characterized by a dissociation constant (Kd) of approximately 3 μM. Determination of the binding energy hot spots was carried out by replacing certain residues that comprise the protein–protein interface with alanine and by evaluating the effect of the mutation on Kd and on the efficiency of core iron mobilization from BfrB. The results identified hot spot residues in both proteins [LB68, EA81, and EA85 in BfrB (superscript for residue number and subscript for chain) and Y2 and L5 in Bfd] that network at the interface to produce a highly complementary hot region for the interaction. The hot spot residues are conserved in the amino acid sequences of Bfr and Bfd proteins from a number of Gram-negative pathogens, indicating that the BfrB:Bfd interaction is of widespread significance in bacterial iron metabolism.},
doi = {10.1021/acs.biochem.5b00937},
journal = {Biochemistry},
number = 40,
volume = 54,
place = {United States},
year = {Fri Sep 25 00:00:00 EDT 2015},
month = {Fri Sep 25 00:00:00 EDT 2015}
}
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