DOE PAGES title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Burkholderia phytofirmans inoculation-induced changes on the shoot cell anatomy and iron accumulation reveal novel components of Arabidopsis-endophyte interaction that can benefit downstream biomass deconstruction

Abstract

In this study, it is known that plant growth promoting bacteria (PGPB) elicit positive effects on plant growth and biomass yield. However, the actual mechanism behind the plant-PGPB interaction is poorly understood, and the literature is scarce regarding the thermochemical pretreatability and enzymatic degradability of biomass derived from PGPB-inoculated plants. Most recent transcriptional analyses of PGPB strain Burkholderia phytofirmans PsJN inoculating potato in literature and Arabidopsis in our present study have revealed the expression of genes for ferritin and the biosynthesis and transport of siderophores (i.e., the molecules with high affinity for iron), respectively. The expression of such genes in the shoots of PsJN-inoculated plants prompted us to propose that PsJN-inoculation can improve the host plant's iron uptake and accumulation, which facilitates the downstream plant biomass pretreatment and conversion to simple sugars. In this study, we employed B. phytofirmans PsJN to inoculate the Arabidopsis thaliana plants, and conducted the first investigation for its effects on the biomass yield, the anatomical organization of stems, the iron accumulation, and the pretreatment and enzymatic hydrolysis of harvested biomass. The results showed that the strain PsJN stimulated plant growth in the earlier period of plant development and enlarged the cell size of stem piths,more » and it also indeed enhanced the essential metals uptake and accumulation in host plants. Moreover, we found that the PsJN-inoculated plant biomass released more glucose and xylose after hot water pretreatment and subsequent co-saccharification, which provided a novel insight into development of lignocellulosic biofuels from renewable biomass resources.« less

Authors:
 [1];  [1];  [1];  [1];  [1];  [1];  [1]
  1. National Renewable Energy Lab. (NREL), Golden, CO (United States)
Publication Date:
Research Org.:
National Renewable Energy Laboratory (NREL), Golden, CO (United States); Energy Frontier Research Centers (EFRC) (United States). Center for Direct Catalytic Conversion of Biomass to Biofuels (C3Bio)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER); USDOE Office of Science (SC), Basic Energy Sciences (BES)
OSTI Identifier:
1240076
Report Number(s):
NREL/JA-2700-64341
Journal ID: ISSN 1664-462X
Grant/Contract Number:  
AC36-08GO28308; SC000997
Resource Type:
Accepted Manuscript
Journal Name:
Frontiers in Plant Science
Additional Journal Information:
Journal Volume: 7; Journal Issue: 3; Related Information: Frontiers in Plant Science; Journal ID: ISSN 1664-462X
Publisher:
Frontiers Research Foundation
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; 59 BASIC BIOLOGICAL SCIENCES; plant growth promoting rhizobacteria (PGPR); plant growth; biomass deconstruction; essential mineral nutrients; Burkholderia phytofirmans; PsJN; iron accumulation; biomass conversion

Citation Formats

Zhao, Shuai, Wei, Hui, Lin, Chien -Yuan, Zeng, Yining, Tucker, Melvin P., Himmel, Michael E., and Ding, Shi -You. Burkholderia phytofirmans inoculation-induced changes on the shoot cell anatomy and iron accumulation reveal novel components of Arabidopsis-endophyte interaction that can benefit downstream biomass deconstruction. United States: N. p., 2016. Web. doi:10.3389/fpls.2016.00024.
Zhao, Shuai, Wei, Hui, Lin, Chien -Yuan, Zeng, Yining, Tucker, Melvin P., Himmel, Michael E., & Ding, Shi -You. Burkholderia phytofirmans inoculation-induced changes on the shoot cell anatomy and iron accumulation reveal novel components of Arabidopsis-endophyte interaction that can benefit downstream biomass deconstruction. United States. https://doi.org/10.3389/fpls.2016.00024
Zhao, Shuai, Wei, Hui, Lin, Chien -Yuan, Zeng, Yining, Tucker, Melvin P., Himmel, Michael E., and Ding, Shi -You. Fri . "Burkholderia phytofirmans inoculation-induced changes on the shoot cell anatomy and iron accumulation reveal novel components of Arabidopsis-endophyte interaction that can benefit downstream biomass deconstruction". United States. https://doi.org/10.3389/fpls.2016.00024. https://www.osti.gov/servlets/purl/1240076.
@article{osti_1240076,
title = {Burkholderia phytofirmans inoculation-induced changes on the shoot cell anatomy and iron accumulation reveal novel components of Arabidopsis-endophyte interaction that can benefit downstream biomass deconstruction},
author = {Zhao, Shuai and Wei, Hui and Lin, Chien -Yuan and Zeng, Yining and Tucker, Melvin P. and Himmel, Michael E. and Ding, Shi -You},
abstractNote = {In this study, it is known that plant growth promoting bacteria (PGPB) elicit positive effects on plant growth and biomass yield. However, the actual mechanism behind the plant-PGPB interaction is poorly understood, and the literature is scarce regarding the thermochemical pretreatability and enzymatic degradability of biomass derived from PGPB-inoculated plants. Most recent transcriptional analyses of PGPB strain Burkholderia phytofirmans PsJN inoculating potato in literature and Arabidopsis in our present study have revealed the expression of genes for ferritin and the biosynthesis and transport of siderophores (i.e., the molecules with high affinity for iron), respectively. The expression of such genes in the shoots of PsJN-inoculated plants prompted us to propose that PsJN-inoculation can improve the host plant's iron uptake and accumulation, which facilitates the downstream plant biomass pretreatment and conversion to simple sugars. In this study, we employed B. phytofirmans PsJN to inoculate the Arabidopsis thaliana plants, and conducted the first investigation for its effects on the biomass yield, the anatomical organization of stems, the iron accumulation, and the pretreatment and enzymatic hydrolysis of harvested biomass. The results showed that the strain PsJN stimulated plant growth in the earlier period of plant development and enlarged the cell size of stem piths, and it also indeed enhanced the essential metals uptake and accumulation in host plants. Moreover, we found that the PsJN-inoculated plant biomass released more glucose and xylose after hot water pretreatment and subsequent co-saccharification, which provided a novel insight into development of lignocellulosic biofuels from renewable biomass resources.},
doi = {10.3389/fpls.2016.00024},
journal = {Frontiers in Plant Science},
number = 3,
volume = 7,
place = {United States},
year = {Fri Jan 29 00:00:00 EST 2016},
month = {Fri Jan 29 00:00:00 EST 2016}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record

Citation Metrics:
Cited by: 17 works
Citation information provided by
Web of Science

Figures / Tables:

TABLE 1 TABLE 1: Forward (F) and reverse (R) primer sequences for real time RT (reverse transcription)-PCR analysis of core genes of B. phytofirmans PsJN related to iron storage and transport.

Save / Share:

Works referenced in this record:

Corn stover pretreatment by inorganic salts and its effects on hemicellulose and cellulose degradation
journal, December 2009


Expression and characterization of Acidothermus cellulolyticus E1 endoglucanase in transgenic duckweed Lemna minor 8627
journal, November 2007


Analysis of Transgenic Glycoside Hydrolases Expressed in Plants: T. reesei CBH I and A. cellulolyticus EI
book, January 2012


Endophytic Colonization of Vitis vinifera L. by Plant Growth-Promoting Bacterium Burkholderia sp. Strain PsJN
journal, April 2005


Complete Genome Sequence of the Plant Growth-Promoting Endophyte Burkholderia phytofirmans Strain PsJN
journal, May 2011

  • Weilharter, A.; Mitter, B.; Shin, M. V.
  • Journal of Bacteriology, Vol. 193, Issue 13
  • DOI: 10.1128/JB.05055-11

Effects and Mechanism of Metal Chloride Salts on Pretreatment and Enzymatic Digestibility of Corn Stover
journal, January 2013

  • Kamireddy, Srinivas R.; Li, Jinbao; Tucker, Melvin
  • Industrial & Engineering Chemistry Research, Vol. 52, Issue 5
  • DOI: 10.1021/ie3019609

Potato cytosine methylation and gene expression changes induced by a beneficial bacterial endophyte, Burkholderia phytofirmans strain PsJN
journal, January 2012


The crystal structure of Dps, a ferritin homolog that binds and protects DNA
journal, April 1998

  • Grant, R. A.; Filman, D. J.; Finkel, S. E.
  • Nature Structural Biology, Vol. 5, Issue 4
  • DOI: 10.1038/nsb0498-294

Expression of l-ornithine Nδ-oxygenase (PvdA) in fluorescent Pseudomonas species: an immunochemical and in silico study
journal, January 2004

  • Putignani, Lorenza; Ambrosi, Cecilia; Ascenzi, Paolo
  • Biochemical and Biophysical Research Communications, Vol. 313, Issue 2
  • DOI: 10.1016/j.bbrc.2003.11.116

Switchgrass Field Performance on Two Soils as Affected by Bacterization of Seedlings with Burkholderia phytofirmans Strain PsJN
journal, September 2014

  • Lowman, J. Scott; Lava-Chavez, Alejandra; Kim-Dura, Seonhwa
  • BioEnergy Research, Vol. 8, Issue 1
  • DOI: 10.1007/s12155-014-9536-3

Heterologous expression of glycosyl hydrolases in planta: a new departure for biofuels
journal, August 2008


ORIGINAL RESEARCH: Lignocellulose recalcitrance screening by integrated high-throughput hydrothermal pretreatment and enzymatic saccharification
journal, April 2010

  • Selig, Michael J.; Tucker, Melvin P.; Sykes, Robert W.
  • Industrial Biotechnology, Vol. 6, Issue 2
  • DOI: 10.1089/ind.2010.0009

Biomass Recalcitrance: Engineering Plants and Enzymes for Biofuels Production
journal, February 2007

  • Himmel, M. E.; Ding, S.-Y.; Johnson, D. K.
  • Science, Vol. 315, Issue 5813, p. 804-807
  • DOI: 10.1126/science.1137016

Mechanisms and applications of plant growth promoting rhizobacteria: Current perspective
journal, January 2014


Lignin plays a negative role in the biochemical process for producing lignocellulosic biofuels
journal, June 2014


Elucidating the role of ferrous ion cocatalyst in enhancing dilute acid pretreatment of lignocellulosic biomass
journal, January 2011

  • Wei, Hui; Donohoe, Bryon S.; Vinzant, Todd B.
  • Biotechnology for Biofuels, Vol. 4, Issue 1
  • DOI: 10.1186/1754-6834-4-48

Increase in 4-Coumaryl Alcohol Units during Lignification in Alfalfa ( Medicago sativa ) Alters the Extractability and Molecular Weight of Lignin
journal, October 2010

  • Ziebell, Angela; Gracom, Kristen; Katahira, Rui
  • Journal of Biological Chemistry, Vol. 285, Issue 50
  • DOI: 10.1074/jbc.M110.137315

Quorum Sensing and Indole-3-Acetic Acid Degradation Play a Role in Colonization and Plant Growth Promotion of Arabidopsis thaliana by Burkholderia phytofirmans PsJN
journal, May 2013

  • Zúñiga, Ana; Poupin, María Josefina; Donoso, Raúl
  • Molecular Plant-Microbe Interactions, Vol. 26, Issue 5
  • DOI: 10.1094/MPMI-10-12-0241-R

Self-Assembly in the Ferritin Nano-Cage Protein Superfamily
journal, August 2011

  • Zhang, Yu; Orner, Brendan P.
  • International Journal of Molecular Sciences, Vol. 12, Issue 8
  • DOI: 10.3390/ijms12085406

Drought stress amelioration in wheat through inoculation with Burkholderia phytofirmans strain PsJN
journal, November 2013

  • Naveed, Muhammad; Hussain, M. Baqir; Zahir, Zahir A.
  • Plant Growth Regulation, Vol. 73, Issue 2
  • DOI: 10.1007/s10725-013-9874-8

Effects of different pretreatment methods on chemical composition of sugarcane bagasse and enzymatic hydrolysis
journal, September 2013


Enhancement of Chilling Resistance of Inoculated Grapevine Plantlets with a Plant Growth-Promoting Rhizobacterium, Burkholderia phytofirmans Strain PsJN
journal, September 2006

  • Ait Barka, E.; Nowak, J.; Clement, C.
  • Applied and Environmental Microbiology, Vol. 72, Issue 11
  • DOI: 10.1128/AEM.01047-06

Applications of free living plant growth-promoting rhizobacteria
journal, August 2004


Oligomer saccharide reduction during dilute acid pretreatment co-catalyzed with Lewis acids on corn stover biomass
journal, January 2013

  • Degenstein, John; Kamireddy, Srinivas Reddy; Tucker, Melvin P.
  • International Journal of Agricultural and Biological Engineering, Vol. 6, Issue 2, 54-62
  • DOI: 10.3965/j.ijabe.20130602.007

Transcriptional Regulation of Grass Secondary Cell Wall Biosynthesis: Playing Catch-Up with Arabidopsis thaliana
journal, January 2012


Transgenic ferritin overproduction enhances thermochemical pretreatments in Arabidopsis
journal, January 2015


Early growth promotion and leaf level physiology changes in Burkholderia phytofirmans strain PsJN inoculated switchgrass
journal, January 2015


Plant growth-promoting rhizobacteria and root system functioning
journal, January 2013

  • Vacheron, Jordan; Desbrosses, Guilhem; Bouffaud, Marie-Lara
  • Frontiers in Plant Science, Vol. 4
  • DOI: 10.3389/fpls.2013.00356

In planta expression of A. cellulolyticus Cel5A endocellulase reduces cell wall recalcitrance in tobacco and maize
journal, January 2011

  • Brunecky, Roman; Selig, Michael J.; Vinzant, Todd B.
  • Biotechnology for Biofuels, Vol. 4, Issue 1
  • DOI: 10.1186/1754-6834-4-1

How Does Plant Cell Wall Nanoscale Architecture Correlate with Enzymatic Digestibility?
journal, November 2012


A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures
journal, July 1962


Engineering plant cell walls: tuning lignin monomer composition for deconstructable biofuel feedstocks or resilient biomaterials
journal, January 2014

  • Ciesielski, Peter N.; Resch, Michael G.; Hewetson, Barron
  • Green Chemistry, Vol. 16, Issue 5, p. 2627-2635
  • DOI: 10.1039/c3gc42422g

Genetic Resources for Maize Cell Wall Biology
journal, November 2009

  • Penning, Bryan W.; Hunter, Charles T.; Tayengwa, Reuben
  • Plant Physiology, Vol. 151, Issue 4
  • DOI: 10.1104/pp.109.136804

Burkholderia phytofirmans sp. nov., a novel plant-associated bacterium with plant-beneficial properties
journal, May 2005

  • Sessitsch, A.
  • INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, Vol. 55, Issue 3
  • DOI: 10.1099/ijs.0.63149-0

Advances in the Application of Plant Growth-Promoting Rhizobacteria in Phytoremediation of Heavy Metals
book, January 2013

  • Tak, Hamid Iqbal; Ahmad, Faheem; Babalola, Olubukola Oluranti
  • Reviews of Environmental Contamination and Toxicology Volume 223
  • DOI: 10.1007/978-1-4614-5577-6_2

Growth promotion and colonization of switchgrass (Panicum virgatum) cv. Alamo by bacterial endophyte Burkholderia phytofirmans strain PsJN
journal, January 2012

  • Kim, Seonhwa; Lowman, Scott; Hou, Guichuan
  • Biotechnology for Biofuels, Vol. 5, Issue 1
  • DOI: 10.1186/1754-6834-5-37

Ferritin: Structure, Gene Regulation, and Cellular Function in Animals, Plants, and Microorganisms
journal, June 1987


The Fragile Fiber1 Kinesin Contributes to Cortical Microtubule-Mediated Trafficking of Cell Wall Components
journal, February 2015

  • Zhu, Chuanmei; Ganguly, Anindya; Baskin, Tobias I.
  • Plant Physiology, Vol. 167, Issue 3
  • DOI: 10.1104/pp.114.251462

Biomass pretreatment: Fundamentals toward application
journal, November 2011


Transcriptome Profiling of the Endophyte Burkholderia phytofirmans PsJN Indicates Sensing of the Plant Environment and Drought Stress
journal, September 2015

  • Sheibani-Tezerji, Raheleh; Rattei, Thomas; Sessitsch, Angela
  • mBio, Vol. 6, Issue 5
  • DOI: 10.1128/mBio.00621-15

Disruption of Mediator rescues the stunted growth of a lignin-deficient Arabidopsis mutant
journal, March 2014

  • Bonawitz, Nicholas D.; Kim, Jeong Im; Tobimatsu, Yuki
  • Nature, Vol. 509, Issue 7500, p. 376-380
  • DOI: 10.1038/nature13084

NIR and Py-mbms coupled with multivariate data analysis as a high-throughput biomass characterization technique: a review
journal, August 2014


Effects of the Plant Growth-Promoting Bacterium Burkholderia phytofirmans PsJN throughout the Life Cycle of Arabidopsis thaliana
journal, July 2013


Increased drought stress resilience of maize through endophytic colonization by Burkholderia phytofirmans PsJN and Enterobacter sp. FD17
journal, January 2014


Role of Ornibactin Biosynthesis in the Virulence ofBurkholderia cepacia: Characterization of pvdA, the Gene Encoding l-OrnithineN5-Oxygenase
journal, September 1999


Advances in the Application of Plant Growth-Promoting Rhizobacteria in Phytoremediation of Heavy Metals
book, January 2013

  • Tak, Hamid Iqbal; Ahmad, Faheem; Babalola, Olubukola Oluranti
  • Reviews of Environmental Contamination and Toxicology Volume 223
  • DOI: 10.1007/978-1-4614-5577-6_2

Enterobacter hormaechei as Plant Growth-Promoting Bacteria for Improvement in Lycopersicum esculentum
journal, February 2021


Switchgrass Field Performance on Two Soils as Affected by Bacterization of Seedlings with Burkholderia phytofirmans Strain PsJN
journal, September 2014

  • Lowman, J. Scott; Lava-Chavez, Alejandra; Kim-Dura, Seonhwa
  • BioEnergy Research, Vol. 8, Issue 1
  • DOI: 10.1007/s12155-014-9536-3

Expression of l-ornithine Nδ-oxygenase (PvdA) in fluorescent Pseudomonas species: an immunochemical and in silico study
journal, January 2004

  • Putignani, Lorenza; Ambrosi, Cecilia; Ascenzi, Paolo
  • Biochemical and Biophysical Research Communications, Vol. 313, Issue 2
  • DOI: 10.1016/j.bbrc.2003.11.116

Transgenic ferritin overproduction enhances thermochemical pretreatments in Arabidopsis
journal, January 2015


Expression and characterization of Acidothermus cellulolyticus E1 endoglucanase in transgenic duckweed Lemna minor 8627
journal, November 2007


Corn stover pretreatment by inorganic salts and its effects on hemicellulose and cellulose degradation
journal, December 2009


Effects of different pretreatment methods on chemical composition of sugarcane bagasse and enzymatic hydrolysis
journal, September 2013


Biomass pretreatment: Fundamentals toward application
journal, November 2011


Lignin plays a negative role in the biochemical process for producing lignocellulosic biofuels
journal, June 2014


Increased drought stress resilience of maize through endophytic colonization by Burkholderia phytofirmans PsJN and Enterobacter sp. FD17
journal, January 2014


Mechanisms and applications of plant growth promoting rhizobacteria: Current perspective
journal, January 2014


Early growth promotion and leaf level physiology changes in Burkholderia phytofirmans strain PsJN inoculated switchgrass
journal, January 2015


Heterologous expression of glycosyl hydrolases in planta: a new departure for biofuels
journal, August 2008


Effects and Mechanism of Metal Chloride Salts on Pretreatment and Enzymatic Digestibility of Corn Stover
journal, January 2013

  • Kamireddy, Srinivas R.; Li, Jinbao; Tucker, Melvin
  • Industrial & Engineering Chemistry Research, Vol. 52, Issue 5
  • DOI: 10.1021/ie3019609

Disruption of Mediator rescues the stunted growth of a lignin-deficient Arabidopsis mutant
journal, March 2014

  • Bonawitz, Nicholas D.; Kim, Jeong Im; Tobimatsu, Yuki
  • Nature, Vol. 509, Issue 7500, p. 376-380
  • DOI: 10.1038/nature13084

ORIGINAL RESEARCH: Lignocellulose recalcitrance screening by integrated high-throughput hydrothermal pretreatment and enzymatic saccharification
journal, April 2010

  • Selig, Michael J.; Tucker, Melvin P.; Sykes, Robert W.
  • Industrial Biotechnology, Vol. 6, Issue 2
  • DOI: 10.1089/ind.2010.0009

Burkholderia phytofirmans PsJN Primes Vitis vinifera L. and Confers a Better Tolerance to Low Nonfreezing Temperatures
journal, February 2012

  • Theocharis, Andreas; Bordiec, Sophie; Fernandez, Olivier
  • Molecular Plant-Microbe Interactions, Vol. 25, Issue 2
  • DOI: 10.1094/mpmi-05-11-0124

Burkholderia phytofirmans sp. nov., a novel plant-associated bacterium with plant-beneficial properties
journal, May 2005

  • Sessitsch, A.
  • INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, Vol. 55, Issue 3
  • DOI: 10.1099/ijs.0.63149-0

Genetic Resources for Maize Cell Wall Biology
journal, November 2009

  • Penning, Bryan W.; Hunter, Charles T.; Tayengwa, Reuben
  • Plant Physiology, Vol. 151, Issue 4
  • DOI: 10.1104/pp.109.136804

The Fragile Fiber1 Kinesin Contributes to Cortical Microtubule-Mediated Trafficking of Cell Wall Components
journal, February 2015

  • Zhu, Chuanmei; Ganguly, Anindya; Baskin, Tobias I.
  • Plant Physiology, Vol. 167, Issue 3
  • DOI: 10.1104/pp.114.251462

A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures
journal, July 1962


Biomass Recalcitrance: Engineering Plants and Enzymes for Biofuels Production
journal, February 2007

  • Himmel, M. E.; Ding, S.-Y.; Johnson, D. K.
  • Science, Vol. 315, Issue 5813, p. 804-807
  • DOI: 10.1126/science.1137016

How Does Plant Cell Wall Nanoscale Architecture Correlate with Enzymatic Digestibility?
journal, November 2012


Ferritin: Structure, Gene Regulation, and Cellular Function in Animals, Plants, and Microorganisms
journal, June 1987


In planta expression of A. cellulolyticus Cel5A endocellulase reduces cell wall recalcitrance in tobacco and maize
journal, January 2011

  • Brunecky, Roman; Selig, Michael J.; Vinzant, Todd B.
  • Biotechnology for Biofuels, Vol. 4, Issue 1
  • DOI: 10.1186/1754-6834-4-1

Elucidating the role of ferrous ion cocatalyst in enhancing dilute acid pretreatment of lignocellulosic biomass
journal, January 2011

  • Wei, Hui; Donohoe, Bryon S.; Vinzant, Todd B.
  • Biotechnology for Biofuels, Vol. 4, Issue 1
  • DOI: 10.1186/1754-6834-4-48

Growth promotion and colonization of switchgrass (Panicum virgatum) cv. Alamo by bacterial endophyte Burkholderia phytofirmans strain PsJN
journal, January 2012

  • Kim, Seonhwa; Lowman, Scott; Hou, Guichuan
  • Biotechnology for Biofuels, Vol. 5, Issue 1
  • DOI: 10.1186/1754-6834-5-37

Effects of the Plant Growth-Promoting Bacterium Burkholderia phytofirmans PsJN throughout the Life Cycle of Arabidopsis thaliana
journal, July 2013


Expression of Trichoderma reesei Exo-Cellobiohydrolase I in Transgenic Tobacco Leaves and Calli
journal, January 1999

  • Dai, Ziyu; Hooker, Brian S.; Quesenberry, Ryan D.
  • Applied Biochemistry and Biotechnology, Vol. 79, Issue 1-3
  • DOI: 10.1385/abab:79:1-3:689

Transcriptional Regulation of Grass Secondary Cell Wall Biosynthesis: Playing Catch-Up with Arabidopsis thaliana
journal, January 2012


NIR and Py-mbms coupled with multivariate data analysis as a high-throughput biomass characterization technique: a review
journal, August 2014


Self-Assembly in the Ferritin Nano-Cage Protein Superfamily
journal, August 2011

  • Zhang, Yu; Orner, Brendan P.
  • International Journal of Molecular Sciences, Vol. 12, Issue 8
  • DOI: 10.3390/ijms12085406

Works referencing / citing this record:

Colonization of Arabidopsis thaliana by Herbaspirillum seropedicae promotes its growth and changes its proteomic profile
journal, August 2019

  • Leandro, Mariana Ramos; Rangel, Patrícia Louzada; dos Santos, Tamires Cruz
  • Plant and Soil, Vol. 443, Issue 1-2
  • DOI: 10.1007/s11104-019-04236-1

Bacteria in the plant tissue culture environment
journal, December 2016

  • Orlikowska, Teresa; Nowak, Katarzyna; Reed, Barbara
  • Plant Cell, Tissue and Organ Culture (PCTOC), Vol. 128, Issue 3
  • DOI: 10.1007/s11240-016-1144-9

Volatile-Mediated Effects Predominate in Paraburkholderia phytofirmans Growth Promotion and Salt Stress Tolerance of Arabidopsis thaliana
journal, November 2016


A New Approach to Modify Plant Microbiomes and Traits by Introducing Beneficial Bacteria at Flowering into Progeny Seeds
journal, January 2017

  • Mitter, Birgit; Pfaffenbichler, Nikolaus; Flavell, Richard
  • Frontiers in Microbiology, Vol. 8
  • DOI: 10.3389/fmicb.2017.00011

Paraburkholderia phytofirmans PsJN-Plants Interaction: From Perception to the Induced Mechanisms
journal, August 2018


Alleviation of Salinity Induced Oxidative Stress in Chenopodium quinoa by Fe Biofortification and Biochar—Endophyte Interaction
journal, January 2020


Figures/Tables have been extracted from DOE-funded journal article accepted manuscripts.