The role of pore geometry in single nanoparticle detection

Davenport, Matthew; Healy, Ken; Pevarnik, Matthew; Teslich, Nick; Cabrini, Stefano; Morrison, Alan P.; Siwy, Zuzanna S.; Letant, Sonia E.

doi:10.1021/nn303126n

Title: The role of pore geometry in single nanoparticle detection

Abstract

In this study, we observe single nanoparticle translocation events via resistive pulse sensing using silicon nitride pores described by a range of lengths and diameters. Pores are prepared by focused ion beam milling in 50 nm-, 100 nm-, and 500 nm-thick silicon nitride membranes with diameters fabricated to accommodate spherical silica nanoparticles with sizes chosen to mimic that of virus particles. In this manner, we are able to characterize the role of pore geometry in three key components of the detection scheme, namely, event magnitude, event duration, and event frequency. We find that the electric field created by the applied voltage and the pore’s geometry is a critical factor. We develop approximations to describe this field, which are verified with computer simulations, and interactions between particles and this field. In so doing, we formulate what we believe to be the first approximation for the magnitude of ionic current blockage that explicitly addresses the invariance of access resistance of solid-state pores during particle translocation. These approximations also provide a suitable foundation for estimating the zeta potential of the particles and/or pore surface when studied in conjunction with event durations. We also verify that translocation achieved by electro-osmostic transport is an effectivemore »« less

Authors:

Davenport, Matthew ^[1]; Healy, Ken ^[2]; Pevarnik, Matthew ^[3]; Teslich, Nick ^[4]; Cabrini, Stefano ^[5]; Morrison, Alan P. ^[6]; Siwy, Zuzanna S. ^[3]; Letant, Sonia E. ^[4]

Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Univ. of California, Irvine, CA (United States)
Univ. of California, Irvine, CA (United States); Univ. College Cork (Ireland)
Univ. of California, Irvine, CA (United States)
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Univ. College Cork (Ireland)

Publication Date:: Wed Aug 22 00:00:00 EDT 2012

Research Org.:: Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)

Sponsoring Org.:: USDOE

OSTI Identifier:: 1239207

Report Number(s):: LLNL-JRNL-564494
Journal ID: ISSN 1936-0851

Grant/Contract Number:: AC52-07NA27344

Resource Type:: Accepted Manuscript

Journal Name:: ACS Nano

Additional Journal Information:: Journal Volume: 6; Journal Issue: 9; Journal ID: ISSN 1936-0851

Publisher:: American Chemical Society

Country of Publication:: United States

Language:: English

Subject:: 77 NANOSCIENCE AND NANOTECHNOLOGY; nanopore; nanoparticle; resistive-pulse; aspect ratio; ion current

Citation Formats


                    Davenport, Matthew, Healy, Ken, Pevarnik, Matthew, Teslich, Nick, Cabrini, Stefano, Morrison, Alan P., Siwy, Zuzanna S., and Letant, Sonia E. The role of pore geometry in single nanoparticle detection.  United States: N. p., 2012. 
Web.  doi:10.1021/nn303126n.

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                    Davenport, Matthew, Healy, Ken, Pevarnik, Matthew, Teslich, Nick, Cabrini, Stefano, Morrison, Alan P., Siwy, Zuzanna S., & Letant, Sonia E. The role of pore geometry in single nanoparticle detection.  United States.  https://doi.org/10.1021/nn303126n

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                    Davenport, Matthew, Healy, Ken, Pevarnik, Matthew, Teslich, Nick, Cabrini, Stefano, Morrison, Alan P., Siwy, Zuzanna S., and Letant, Sonia E. Wed .  
"The role of pore geometry in single nanoparticle detection".  United States.  https://doi.org/10.1021/nn303126n.  https://www.osti.gov/servlets/purl/1239207.

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@article{osti_1239207,

  title        = {The role of pore geometry in single nanoparticle detection},

  author       = {Davenport, Matthew and Healy, Ken and Pevarnik, Matthew and Teslich, Nick and Cabrini, Stefano and Morrison, Alan P. and Siwy, Zuzanna S. and Letant, Sonia E.},

  abstractNote = {In this study, we observe single nanoparticle translocation events via resistive pulse sensing using silicon nitride pores described by a range of lengths and diameters. Pores are prepared by focused ion beam milling in 50 nm-, 100 nm-, and 500 nm-thick silicon nitride membranes with diameters fabricated to accommodate spherical silica nanoparticles with sizes chosen to mimic that of virus particles. In this manner, we are able to characterize the role of pore geometry in three key components of the detection scheme, namely, event magnitude, event duration, and event frequency. We find that the electric field created by the applied voltage and the pore’s geometry is a critical factor. We develop approximations to describe this field, which are verified with computer simulations, and interactions between particles and this field. In so doing, we formulate what we believe to be the first approximation for the magnitude of ionic current blockage that explicitly addresses the invariance of access resistance of solid-state pores during particle translocation. These approximations also provide a suitable foundation for estimating the zeta potential of the particles and/or pore surface when studied in conjunction with event durations. We also verify that translocation achieved by electro-osmostic transport is an effective means of slowing translocation velocities of highly charged particles without compromising particle capture rate as compared to more traditional approaches based on electrophoretic transport.},

  doi          = {10.1021/nn303126n},

  journal      = {ACS Nano},

  number       = 9,

  volume       = 6,

  place        = {United States},

  year         = {Wed Aug 22 00:00:00 EDT 2012},

  month        = {Wed Aug 22 00:00:00 EDT 2012}

}

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Title: The role of pore geometry in single nanoparticle detection

Abstract

Citation Formats

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Nanoparticle Transport in Conical-Shaped Nanopores journal, May 2011

The potential and challenges of nanopore sequencing journal, October 2008

Automated forward and reverse ratcheting of DNA in a nanopore at 5-Å precision journal, February 2012

Translocation of Single-Wall Carbon Nanotubes Through Solid-State Nanopores journal, June 2011

Electroosmotic Flow Can Generate Ion Current Rectification in Nano- and Micropores journal, December 2009

Detection of Nucleosomal Substructures using Solid-State Nanopores journal, May 2012

The zeta potential of silicon nitride thin films journal, March 1991

Fabrication of Metallized Nanopores in Silicon Nitride Membranes for Single-Molecule Sensing journal, June 2010

Electrically Facilitated Translocations of Proteins through Silicon Nitride Nanopores: Conjoint and Competitive Action of Diffusion, Electrophoresis, and Electroosmosis journal, June 2010

Polymer capture by electro-osmotic flow of oppositely charged nanopores journal, April 2007

Pressure-Driven Nanoparticle Transport across Glass Membranes Containing a Conical-Shaped Nanopore journal, August 2011

Characterization of Hepatitis B Virus Capsids by Resistive-Pulse Sensing journal, February 2011

Fabrication and Characterization of Single Pores for Modeling Mass Transport across Porous Membranes journal, February 1999

Resistive-Pulse Sensing - From Microbes to Molecules journal, July 2000

Solid-state nanopore technologies for nanopore-based DNA analysis journal, December 2007

Diffusional Motion of a Particle Translocating through a Nanopore journal, January 2012

Access resistance of a small circular pore journal, October 1975

Counting and Sizing of Submicron Particles by the Resistive Pulse Technique journal, July 1970

Simultaneous Size and ζ-Potential Measurements of Individual Nanoparticles in Dispersion Using Size-Tunable Pore Sensors journal, July 2012

Electrostatic focusing of unlabelled DNA into nanoscale pores using a salt gradient journal, December 2009

Inverse Statistical Variates journal, April 1945

Comparison of Nanoparticle Size and Electrophoretic Mobility Measurements Using a Carbon-Nanotube-Based Coulter Counter, Dynamic Light Scattering, Transmission Electron Microscopy, and Phase Analysis Light Scattering journal, August 2004

Single Carbon Nanotube Membranes: A Well-Defined Model for Studying Mass Transport through Nanoporous Materials journal, December 2000

Sizes and concentrations of several type C oncornaviruses and bacteriophage T2 by the resistive-pulse technique. journal, January 1977

The raspberry model for protein-like particles: Ellipsoids and confinement in cylindrical pores journal, October 2016

Controlled size reduction and its underlying mechanism to form solid-state nanopores via electron beam induced carbon deposition journal, August 2019

Low aspect ratio micropores for single-particle and single-cell analysis: Nanoanalysis journal, April 2015

Optimal voltage for nanoparticle detection with thin nanopores journal, January 2018

Effects of access resistance on the resistive-pulse caused by translocating of a nanoparticle through a nanopore journal, January 2014

Electrokinetic motion of single nanoparticles in single PDMS nanochannels journal, January 2017

Characterization and manipulation of single nanoparticles using a nanopore-based electrokinetic tweezer journal, January 2019

Voltage-Gated Nanoparticle Transport and Collisions in Attoliter-Volume Nanopore Electrode Arrays journal, January 2018

Tracking single-particle dynamics via combined optical and electrical sensing journal, May 2013

Volume discrimination of nanoparticles via electrical trapping using nanopores journal, March 2019

Rapid structural analysis of nanomaterials in aqueous solutions journal, March 2017

Nanopore‐Based Protein Sequencing Using Biopores: Current Achievements and Open Challenges journal, February 2020

Probing Dynamic Events of Dielectric Nanoparticles by a Nanoelectrode‐Nanopore Nanopipette journal, May 2018

Discriminating single-bacterial shape using low-aspect-ratio pores journal, December 2017

Microfluidic and Nanofluidic Resistive Pulse Sensing: A Review journal, June 2017

Surface coatings for solid-state nanopores journal, January 2019

The Raspberry model for protein-like particles: ellipsoids and confinement in cylindrical pores text, January 2016

Construction of biomimetic smart nanochannels for confined water journal, November 2013

Detection of Individual Molecules and Ions by Carbon Nanotube-Based Differential Resistive Pulse Sensor journal, March 2018

Dynamics of polymer nanoparticles through a single artificial nanopore with a high-aspect-ratio journal, January 2014

Optimal Voltage for Nanoparticle Detection with Thin Nanopores text, January 2018

Nanopore-based analysis of biochemical species journal, July 2015

Single-Particle Electrophoresis in Nanochannels journal, December 2014

Discriminating single-bacterial shape using low-aspect-ratio pores journal, December 2017

Volume discrimination of nanoparticles via electrical trapping using nanopores journal, March 2019

Microfluidic and Nanofluidic Resistive Pulse Sensing: A Review journal, June 2017

Simultaneous Determination of the Size and Surface Charge of Individual Nanoparticles Using a Carbon Nanotube-Based Coulter Counter
journal, May 2003

Nanopore-based single-molecule DNA analysis
journal, August 2007

Distinguishing Single- and Double-Stranded Nucleic Acid Molecules Using Solid-State Nanopores
journal, August 2009

Solid-state nanopores
journal, March 2007

Dynamics of Colloids in Single Solid-State Nanopores
journal, March 2011

Polystyrene Particles Reveal Pore Substructure As They Translocate
journal, July 2012

DNA Translocation Governed by Interactions with Solid-State Nanopores
journal, November 2008

Electrical Counting and Sizing of Mammalian Cells in Suspension
journal, July 1965

Gate Manipulation of DNA Capture into Nanopores
journal, September 2011

Single-Nanoparticle Detection Using a Low-Aspect-Ratio Pore
journal, March 2012

Transport phenomena in nanofluidics
journal, July 2008

Reading DNA at single-nucleotide resolution with a mutant MspA nanopore and phi29 DNA polymerase
journal, March 2012

Nanopores: A journey towards DNA sequencing
journal, June 2012

Submicrometer Pore-Based Characterization and Quantification of Antibody–Virus Interactions
journal, August 2006

Ionic Field Effect Transistors with Sub-10 nm Multiple Nanopores
journal, May 2009

Characterization of individual polynucleotide molecules using a membrane channel
journal, November 1996

Sensing protein molecules using nanofabricated pores
journal, February 2006

Nanopore sensors for nucleic acid analysis
journal, September 2011

Electrokinetic measurements with submicron particles and pores by the resistive pulse technique
journal, September 1977

Quantitative sensing of nanoscale colloids using a microchip Coulter counter
journal, December 2001

Nanoparticle Transport in Conical-Shaped Nanopores
journal, May 2011

The potential and challenges of nanopore sequencing
journal, October 2008

Automated forward and reverse ratcheting of DNA in a nanopore at 5-Å precision
journal, February 2012

Translocation of Single-Wall Carbon Nanotubes Through Solid-State Nanopores
journal, June 2011

Electroosmotic Flow Can Generate Ion Current Rectification in Nano- and Micropores
journal, December 2009

Detection of Nucleosomal Substructures using Solid-State Nanopores
journal, May 2012

The zeta potential of silicon nitride thin films
journal, March 1991

Fabrication of Metallized Nanopores in Silicon Nitride Membranes for Single-Molecule Sensing
journal, June 2010

Electrically Facilitated Translocations of Proteins through Silicon Nitride Nanopores: Conjoint and Competitive Action of Diffusion, Electrophoresis, and Electroosmosis
journal, June 2010

Polymer capture by electro-osmotic flow of oppositely charged nanopores
journal, April 2007

Pressure-Driven Nanoparticle Transport across Glass Membranes Containing a Conical-Shaped Nanopore
journal, August 2011

Characterization of Hepatitis B Virus Capsids by Resistive-Pulse Sensing
journal, February 2011

Fabrication and Characterization of Single Pores for Modeling Mass Transport across Porous Membranes
journal, February 1999

Resistive-Pulse Sensing - From Microbes to Molecules
journal, July 2000

Solid-state nanopore technologies for nanopore-based DNA analysis
journal, December 2007

Diffusional Motion of a Particle Translocating through a Nanopore
journal, January 2012

Access resistance of a small circular pore
journal, October 1975

Counting and Sizing of Submicron Particles by the Resistive Pulse Technique
journal, July 1970

Simultaneous Size and ζ-Potential Measurements of Individual Nanoparticles in Dispersion Using Size-Tunable Pore Sensors
journal, July 2012

Electrostatic focusing of unlabelled DNA into nanoscale pores using a salt gradient
journal, December 2009

Inverse Statistical Variates
journal, April 1945

Comparison of Nanoparticle Size and Electrophoretic Mobility Measurements Using a Carbon-Nanotube-Based Coulter Counter, Dynamic Light Scattering, Transmission Electron Microscopy, and Phase Analysis Light Scattering
journal, August 2004

Single Carbon Nanotube Membranes: A Well-Defined Model for Studying Mass Transport through Nanoporous Materials
journal, December 2000

Sizes and concentrations of several type C oncornaviruses and bacteriophage T2 by the resistive-pulse technique.
journal, January 1977

The raspberry model for protein-like particles: Ellipsoids and confinement in cylindrical pores
journal, October 2016

Controlled size reduction and its underlying mechanism to form solid-state nanopores via electron beam induced carbon deposition
journal, August 2019

Low aspect ratio micropores for single-particle and single-cell analysis: Nanoanalysis
journal, April 2015

Optimal voltage for nanoparticle detection with thin nanopores
journal, January 2018

Effects of access resistance on the resistive-pulse caused by translocating of a nanoparticle through a nanopore
journal, January 2014

Electrokinetic motion of single nanoparticles in single PDMS nanochannels
journal, January 2017

Characterization and manipulation of single nanoparticles using a nanopore-based electrokinetic tweezer
journal, January 2019

Voltage-Gated Nanoparticle Transport and Collisions in Attoliter-Volume Nanopore Electrode Arrays
journal, January 2018

Tracking single-particle dynamics via combined optical and electrical sensing
journal, May 2013

Volume discrimination of nanoparticles via electrical trapping using nanopores
journal, March 2019

Rapid structural analysis of nanomaterials in aqueous solutions
journal, March 2017

Nanopore‐Based Protein Sequencing Using Biopores: Current Achievements and Open Challenges
journal, February 2020

Probing Dynamic Events of Dielectric Nanoparticles by a Nanoelectrode‐Nanopore Nanopipette
journal, May 2018

Discriminating single-bacterial shape using low-aspect-ratio pores
journal, December 2017

Microfluidic and Nanofluidic Resistive Pulse Sensing: A Review
journal, June 2017

Surface coatings for solid-state nanopores
journal, January 2019

The Raspberry model for protein-like particles: ellipsoids and confinement in cylindrical pores
text, January 2016

Construction of biomimetic smart nanochannels for confined water
journal, November 2013

Detection of Individual Molecules and Ions by Carbon Nanotube-Based Differential Resistive Pulse Sensor
journal, March 2018

Dynamics of polymer nanoparticles through a single artificial nanopore with a high-aspect-ratio
journal, January 2014

Optimal Voltage for Nanoparticle Detection with Thin Nanopores
text, January 2018

Nanopore-based analysis of biochemical species
journal, July 2015

Single-Particle Electrophoresis in Nanochannels
journal, December 2014

Discriminating single-bacterial shape using low-aspect-ratio pores
journal, December 2017

Volume discrimination of nanoparticles via electrical trapping using nanopores
journal, March 2019

Microfluidic and Nanofluidic Resistive Pulse Sensing: A Review
journal, June 2017