Crystal structures of the components of the Staphylococcus aureus leukotoxin ED
Abstract
Staphylococcal leukotoxins are a family of β-barrel, bicomponent, pore-forming toxins with membrane-damaging functions. These bacterial exotoxins share sequence and structural homology and target several host-cell types. Leukotoxin ED (LukED) is one of these bicomponent pore-forming toxins thatStaphylococcus aureusproduces in order to suppress the ability of the host to contain the infection. The recent delineation of the important role that LukED plays inS. aureuspathogenesis and the identification of its protein receptors, combined with its presence inS. aureusmethicillin-resistant epidemic strains, establish this leukocidin as a possible target for the development of novel therapeutics. Here, the crystal structures of the water-soluble LukE and LukD components of LukED have been determined. Lastly, the two structures illustrate the tertiary-structural variability with respect to the other leukotoxins while retaining the conservation of the residues involved in the interaction of the protomers in the bipartite leukotoxin in the pore complex.
- Authors:
-
- Northwestern Univ., Chicago, IL (United States). Feinberg School of Medicine, Center for Structural Genomics of Infectious Diseases, Dept. of Biochemistry and Molecular Genetics
- Novartis Vaccines and Diagnostics, Research Centre, Siena (Italy)
- Publication Date:
- Research Org.:
- Argonne National Laboratory (ANL), Argonne, IL (United States). Advanced Photon Source (APS)
- Sponsoring Org.:
- USDOE; National Institutes of Health (NIH)
- OSTI Identifier:
- 1233746
- Grant/Contract Number:
- AC02-06CH11357; HHSN272200700058C; HHSN272201200026C
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Acta Crystallographica. Section D. Structural Biology
- Additional Journal Information:
- Journal Volume: 72; Journal Issue: 1; Journal ID: ISSN 2059-7983
- Publisher:
- IUCr
- Country of Publication:
- United States
- Language:
- ENGLISH
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES; LukE; LukD; leukotoxin; poreforming toxins; Staphylococcus aureus
Citation Formats
Nocadello, S., Minasov, G., Shuvalova, L., Dubrovska, I., Sabini, E., Bagnoli, F., Grandi, G., and Anderson, W. F. Crystal structures of the components of the Staphylococcus aureus leukotoxin ED. United States: N. p., 2016.
Web. doi:10.1107/S2059798315023207.
Nocadello, S., Minasov, G., Shuvalova, L., Dubrovska, I., Sabini, E., Bagnoli, F., Grandi, G., & Anderson, W. F. Crystal structures of the components of the Staphylococcus aureus leukotoxin ED. United States. https://doi.org/10.1107/S2059798315023207
Nocadello, S., Minasov, G., Shuvalova, L., Dubrovska, I., Sabini, E., Bagnoli, F., Grandi, G., and Anderson, W. F. Fri .
"Crystal structures of the components of the Staphylococcus aureus leukotoxin ED". United States. https://doi.org/10.1107/S2059798315023207. https://www.osti.gov/servlets/purl/1233746.
@article{osti_1233746,
title = {Crystal structures of the components of the Staphylococcus aureus leukotoxin ED},
author = {Nocadello, S. and Minasov, G. and Shuvalova, L. and Dubrovska, I. and Sabini, E. and Bagnoli, F. and Grandi, G. and Anderson, W. F.},
abstractNote = {Staphylococcal leukotoxins are a family of β-barrel, bicomponent, pore-forming toxins with membrane-damaging functions. These bacterial exotoxins share sequence and structural homology and target several host-cell types. Leukotoxin ED (LukED) is one of these bicomponent pore-forming toxins thatStaphylococcus aureusproduces in order to suppress the ability of the host to contain the infection. The recent delineation of the important role that LukED plays inS. aureuspathogenesis and the identification of its protein receptors, combined with its presence inS. aureusmethicillin-resistant epidemic strains, establish this leukocidin as a possible target for the development of novel therapeutics. Here, the crystal structures of the water-soluble LukE and LukD components of LukED have been determined. Lastly, the two structures illustrate the tertiary-structural variability with respect to the other leukotoxins while retaining the conservation of the residues involved in the interaction of the protomers in the bipartite leukotoxin in the pore complex.},
doi = {10.1107/S2059798315023207},
journal = {Acta Crystallographica. Section D. Structural Biology},
number = 1,
volume = 72,
place = {United States},
year = {Fri Jan 01 00:00:00 EST 2016},
month = {Fri Jan 01 00:00:00 EST 2016}
}
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Works referencing / citing this record:
Identification of LukPQ, a novel, equid-adapted leukocidin of Staphylococcus aureus
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