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Title: Evolution of plant δ1-pyrroline-5-carboxylate reductases from phylogenetic and structural perspectives

Abstract

Proline plays a crucial role in cell growth and stress responses, and its accumulation is essential for the tolerance of adverse environmental conditions in plants. Two routes are used to biosynthesize proline in plants. The main route uses glutamate as a precursor, while in the other route proline is derived from ornithine. The terminal step of both pathways, the conversion of δ1-pyrroline-5-carboxylate (P5C) to L-proline, is catalyzed by P5C reductase (P5CR) using NADH or NADPH as a cofactor. Since P5CRs are important housekeeping enzymes, they are conserved across all domains of life and appear to be relatively unaffected throughout evolution. However, global analysis of these enzymes unveiled significant functional diversity in the preference for cofactors (NADPH vs. NADH), variation in metal dependence and the differences in the oligomeric state. In our study we investigated evolutionary patterns through phylogenetic and structural analysis of P5CR representatives from all kingdoms of life, with emphasis on the plant species. We attempted to correlate local sequence/structure variation among the functionally and structurally characterized members of the family.

Authors:
 [1];  [2];  [3];  [1];  [3]
  1. Univ. of Ferrara, Ferrara (Italy)
  2. National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD (United States)
  3. Argonne National Lab. (ANL), Argonne, IL (United States)
Publication Date:
Research Org.:
Argonne National Laboratory (ANL), Argonne, IL (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1224096
Grant/Contract Number:  
AC02-06CH11357
Resource Type:
Accepted Manuscript
Journal Name:
Frontiers in Plant Science
Additional Journal Information:
Journal Volume: 6; Journal ID: ISSN 1664-462X
Publisher:
Frontiers Research Foundation
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; P5C reductase; phylogenetic analysis; 3-D structures of P5CRs; oligomer structure prediction; cofactor preference

Citation Formats

Forlani, Giuseppe, Makarova, Kira S., Ruszkowski, Milosz, Bertazzini, Michele, and Nocek, Boguslaw. Evolution of plant δ1-pyrroline-5-carboxylate reductases from phylogenetic and structural perspectives. United States: N. p., 2015. Web. doi:10.3389/fpls.2015.00567.
Forlani, Giuseppe, Makarova, Kira S., Ruszkowski, Milosz, Bertazzini, Michele, & Nocek, Boguslaw. Evolution of plant δ1-pyrroline-5-carboxylate reductases from phylogenetic and structural perspectives. United States. https://doi.org/10.3389/fpls.2015.00567
Forlani, Giuseppe, Makarova, Kira S., Ruszkowski, Milosz, Bertazzini, Michele, and Nocek, Boguslaw. Mon . "Evolution of plant δ1-pyrroline-5-carboxylate reductases from phylogenetic and structural perspectives". United States. https://doi.org/10.3389/fpls.2015.00567. https://www.osti.gov/servlets/purl/1224096.
@article{osti_1224096,
title = {Evolution of plant δ1-pyrroline-5-carboxylate reductases from phylogenetic and structural perspectives},
author = {Forlani, Giuseppe and Makarova, Kira S. and Ruszkowski, Milosz and Bertazzini, Michele and Nocek, Boguslaw},
abstractNote = {Proline plays a crucial role in cell growth and stress responses, and its accumulation is essential for the tolerance of adverse environmental conditions in plants. Two routes are used to biosynthesize proline in plants. The main route uses glutamate as a precursor, while in the other route proline is derived from ornithine. The terminal step of both pathways, the conversion of δ1-pyrroline-5-carboxylate (P5C) to L-proline, is catalyzed by P5C reductase (P5CR) using NADH or NADPH as a cofactor. Since P5CRs are important housekeeping enzymes, they are conserved across all domains of life and appear to be relatively unaffected throughout evolution. However, global analysis of these enzymes unveiled significant functional diversity in the preference for cofactors (NADPH vs. NADH), variation in metal dependence and the differences in the oligomeric state. In our study we investigated evolutionary patterns through phylogenetic and structural analysis of P5CR representatives from all kingdoms of life, with emphasis on the plant species. We attempted to correlate local sequence/structure variation among the functionally and structurally characterized members of the family.},
doi = {10.3389/fpls.2015.00567},
journal = {Frontiers in Plant Science},
number = ,
volume = 6,
place = {United States},
year = {Mon Aug 03 00:00:00 EDT 2015},
month = {Mon Aug 03 00:00:00 EDT 2015}
}

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Cited by: 18 works
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Requirement of proline synthesis during Arabidopsis reproductive development
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Pyrroline-5-Carboxylate Reductase Is in Pea ( Pisum sativum L.) Leaf Chloroplasts
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Works referencing / citing this record:

The structure of Medicago truncatula δ1-pyrroline-5-carboxylate reductase provides new insights into regulation of proline biosynthesis in plants
journal, October 2015

  • Ruszkowski, Milosz; Nocek, Boguslaw; Forlani, Giuseppe
  • Frontiers in Plant Science, Vol. 6
  • DOI: 10.3389/fpls.2015.00869

Functional Characterization of Four Putative δ1-Pyrroline-5-Carboxylate Reductases from Bacillus subtilis
journal, August 2017

  • Forlani, Giuseppe; Nocek, Boguslaw; Chakravarthy, Srinivas
  • Frontiers in Microbiology, Vol. 8
  • DOI: 10.3389/fmicb.2017.01442