Serial femtosecond X-ray diffraction of enveloped virus microcrystals
Abstract
Serial femtosecond crystallography (SFX) using X-ray free-electron lasers has produced high-resolution, room temperature, time-resolved protein structures. We report preliminary SFX of Sindbis virus, an enveloped icosahedral RNA virus with ~700 Å diameter. Microcrystals delivered in viscous agarose medium diffracted to ~40 Å resolution. Small-angle diffuse X-ray scattering overlaid Bragg peaks and analysis suggests this results from molecular transforms of individual particles. Viral proteins undergo structural changes during entry and infection, which could, in principle, be studied with SFX. This is a pertinent step toward determining room temperature structures from virus microcrystals that may enable time-resolved studies of enveloped viruses.
- Authors:
-
- Arizona State Univ., Tempe, AZ (United States)
- State Univ. of New York, Buffalo, NY (United States)
- Arizona State Univ., Tempe, AZ (United States); Beijing Computational Science Research Center, Beijing (China)
- SLAC National Accelerator Lab., Menlo Park, CA (United States)
- Univ. of Southern California, Los Angeles, CA (United States)
- Publication Date:
- Research Org.:
- SLAC National Accelerator Laboratory (SLAC), Menlo Park, CA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC)
- OSTI Identifier:
- 1222954
- Grant/Contract Number:
- AC03-76SF00515
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Structural Dynamics
- Additional Journal Information:
- Journal Volume: 2; Journal Issue: 4; Journal ID: ISSN 2329-7778
- Publisher:
- American Crystallographic Association/AIP
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; viruses; x-ray scattering; proteins; x-ray diffraction; crystal structure
Citation Formats
Lawrence, Robert M., Conrad, Chelsie E., Zatsepin, Nadia A., Grant, Thomas D., Liu, Haiguang, James, Daniel, Nelson, Garrett, Subramanian, Ganesh, Aquila, Andrew, Hunter, Mark S., Liang, Mengning, Boutet, Sébastien, Coe, Jesse, Spence, John C. H., Weierstall, Uwe, Liu, Wei, Fromme, Petra, Cherezov, Vadim, and Hogue, Brenda G. Serial femtosecond X-ray diffraction of enveloped virus microcrystals. United States: N. p., 2015.
Web. doi:10.1063/1.4929410.
Lawrence, Robert M., Conrad, Chelsie E., Zatsepin, Nadia A., Grant, Thomas D., Liu, Haiguang, James, Daniel, Nelson, Garrett, Subramanian, Ganesh, Aquila, Andrew, Hunter, Mark S., Liang, Mengning, Boutet, Sébastien, Coe, Jesse, Spence, John C. H., Weierstall, Uwe, Liu, Wei, Fromme, Petra, Cherezov, Vadim, & Hogue, Brenda G. Serial femtosecond X-ray diffraction of enveloped virus microcrystals. United States. https://doi.org/10.1063/1.4929410
Lawrence, Robert M., Conrad, Chelsie E., Zatsepin, Nadia A., Grant, Thomas D., Liu, Haiguang, James, Daniel, Nelson, Garrett, Subramanian, Ganesh, Aquila, Andrew, Hunter, Mark S., Liang, Mengning, Boutet, Sébastien, Coe, Jesse, Spence, John C. H., Weierstall, Uwe, Liu, Wei, Fromme, Petra, Cherezov, Vadim, and Hogue, Brenda G. Thu .
"Serial femtosecond X-ray diffraction of enveloped virus microcrystals". United States. https://doi.org/10.1063/1.4929410. https://www.osti.gov/servlets/purl/1222954.
@article{osti_1222954,
title = {Serial femtosecond X-ray diffraction of enveloped virus microcrystals},
author = {Lawrence, Robert M. and Conrad, Chelsie E. and Zatsepin, Nadia A. and Grant, Thomas D. and Liu, Haiguang and James, Daniel and Nelson, Garrett and Subramanian, Ganesh and Aquila, Andrew and Hunter, Mark S. and Liang, Mengning and Boutet, Sébastien and Coe, Jesse and Spence, John C. H. and Weierstall, Uwe and Liu, Wei and Fromme, Petra and Cherezov, Vadim and Hogue, Brenda G.},
abstractNote = {Serial femtosecond crystallography (SFX) using X-ray free-electron lasers has produced high-resolution, room temperature, time-resolved protein structures. We report preliminary SFX of Sindbis virus, an enveloped icosahedral RNA virus with ~700 Å diameter. Microcrystals delivered in viscous agarose medium diffracted to ~40 Å resolution. Small-angle diffuse X-ray scattering overlaid Bragg peaks and analysis suggests this results from molecular transforms of individual particles. Viral proteins undergo structural changes during entry and infection, which could, in principle, be studied with SFX. This is a pertinent step toward determining room temperature structures from virus microcrystals that may enable time-resolved studies of enveloped viruses.},
doi = {10.1063/1.4929410},
journal = {Structural Dynamics},
number = 4,
volume = 2,
place = {United States},
year = {Thu Aug 20 00:00:00 EDT 2015},
month = {Thu Aug 20 00:00:00 EDT 2015}
}
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