Acoustic transfer of protein crystals from agarose pedestals to micromeshes for high-throughput screening

Cuttitta, Christina M.; Ericson, Daniel L.; Scalia, Alexander; Roessler, Christian G.; Teplitsky, Ella; Joshi, Karan; Campos, Olven; Agarwal, Rakhi; Allaire, Marc; Orville, Allen M.; Sweet, Robert M.; Soares, Alexei S.

doi:10.1107/S1399004714013728

Title: Acoustic transfer of protein crystals from agarose pedestals to micromeshes for high-throughput screening

Abstract

Acoustic droplet ejection (ADE) is an emerging technology with broad applications in serial crystallography such as growing, improving and manipulating protein crystals. One application of this technology is to gently transfer crystals onto MiTeGen micromeshes with minimal solvent. Once mounted on a micromesh, each crystal can be combined with different chemicals such as crystal-improving additives or a fragment library. Acoustic crystal mounting is fast (2.33 transfers s^-1) and all transfers occur in a sealed environment that is in vapor equilibrium with the mother liquor. Here, a system is presented to retain crystals near the ejection point and away from the inaccessible dead volume at the bottom of the well by placing the crystals on a concave agarose pedestal (CAP) with the same chemical composition as the crystal mother liquor. The bowl-shaped CAP is impenetrable to crystals. Consequently, gravity will gently move the crystals into the optimal location for acoustic ejection. It is demonstrated that an agarose pedestal of this type is compatible with most commercially available crystallization conditions and that protein crystals are readily transferred from the agarose pedestal onto micromeshes with no loss in diffraction quality. It is also shown that crystals can be grown directly on CAPs, whichmore »« less

Authors:

Cuttitta, Christina M. ^[1]; Ericson, Daniel L. ^[2]; Scalia, Alexander ^[3]; Roessler, Christian G. ^[4]; Teplitsky, Ella ^[5]; Joshi, Karan ^[6]; Campos, Olven ^[7]; Agarwal, Rakhi ^[8]; Allaire, Marc ^[4]; Orville, Allen M. ^[4]; Sweet, Robert M. ^[4]; Soares, Alexei S. ^[4]

Brookhaven National Lab. (BNL), Upton, NY (United States). Office of Educational Programs; City Univ. of New York, Staten Island, NY (United States); Center for Developmental Neuroscience and Dept. of Biology
Brookhaven National Lab. (BNL), Upton, NY (United States). Office of Educational Programs; Univ. at Buffalo, SUNY, NY (United States). Dept. of Biomedical Engineering
Brookhaven National Lab. (BNL), Upton, NY (United States). Office of Educational Programs; Binghamton Univ., NY (United States). Dept. of Biological Sciences
Brookhaven National Lab. (BNL), Upton, NY (United States). Photon Sciences Directorate
Brookhaven National Lab. (BNL), Upton, NY (United States). Office of Educational Programs; Stony Brook Univ., NY (United States). Dept. of Biochemistry and Cell Biology
Brookhaven National Lab. (BNL), Upton, NY (United States). Office of Educational Programs; PEC Univ. of Technology, Chandigarh (India). Dept. of Electronics and Electrical Communication Engineering
Brookhaven National Lab. (BNL), Upton, NY (United States). Office of Educational Programs; Florida Atlantic Univ., Boca Raton, FL (United States). Dept. of Biological Science
Brookhaven National Lab. (BNL), Upton, NY (United States). Biosciences Dept.

Publication Date:: Sun Jun 01 00:00:00 EDT 2014

Research Org.:: Brookhaven National Laboratory (BNL), Upton, NY (United States)

Sponsoring Org.:: USDOE Office of Science (SC)

OSTI Identifier:: 1210164

Report Number(s):: BNL-107952-2015-JA
Journal ID: ISSN 1399-0047; ABCRE6; R&D Project: LS001

Grant/Contract Number:: SC00112704

Resource Type:: Accepted Manuscript

Journal Name:: Acta Crystallographica. Section D: Biological Crystallography (Online)

Additional Journal Information:: Journal Name: Acta Crystallographica. Section D: Biological Crystallography (Online); Journal Volume: 71; Journal Issue: 1; Journal ID: ISSN 1399-0047

Publisher:: International Union of Crystallography

Country of Publication:: United States

Language:: English

Subject:: 36 MATERIALS SCIENCE

Citation Formats


                    Cuttitta, Christina M., Ericson, Daniel L., Scalia, Alexander, Roessler, Christian G., Teplitsky, Ella, Joshi, Karan, Campos, Olven, Agarwal, Rakhi, Allaire, Marc, Orville, Allen M., Sweet, Robert M., and Soares, Alexei S. Acoustic transfer of protein crystals from agarose pedestals to micromeshes for high-throughput screening.  United States: N. p., 2014. 
Web.  doi:10.1107/S1399004714013728.

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                    Cuttitta, Christina M., Ericson, Daniel L., Scalia, Alexander, Roessler, Christian G., Teplitsky, Ella, Joshi, Karan, Campos, Olven, Agarwal, Rakhi, Allaire, Marc, Orville, Allen M., Sweet, Robert M., & Soares, Alexei S. Acoustic transfer of protein crystals from agarose pedestals to micromeshes for high-throughput screening.  United States.  https://doi.org/10.1107/S1399004714013728

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                    Cuttitta, Christina M., Ericson, Daniel L., Scalia, Alexander, Roessler, Christian G., Teplitsky, Ella, Joshi, Karan, Campos, Olven, Agarwal, Rakhi, Allaire, Marc, Orville, Allen M., Sweet, Robert M., and Soares, Alexei S. Sun .  
"Acoustic transfer of protein crystals from agarose pedestals to micromeshes for high-throughput screening".  United States.  https://doi.org/10.1107/S1399004714013728.  https://www.osti.gov/servlets/purl/1210164.

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@article{osti_1210164,

  title        = {Acoustic transfer of protein crystals from agarose pedestals to micromeshes for high-throughput screening},

  author       = {Cuttitta, Christina M. and Ericson, Daniel L. and Scalia, Alexander and Roessler, Christian G. and Teplitsky, Ella and Joshi, Karan and Campos, Olven and Agarwal, Rakhi and Allaire, Marc and Orville, Allen M. and Sweet, Robert M. and Soares, Alexei S.},

  abstractNote = {Acoustic droplet ejection (ADE) is an emerging technology with broad applications in serial crystallography such as growing, improving and manipulating protein crystals. One application of this technology is to gently transfer crystals onto MiTeGen micromeshes with minimal solvent. Once mounted on a micromesh, each crystal can be combined with different chemicals such as crystal-improving additives or a fragment library. Acoustic crystal mounting is fast (2.33 transfers s-1) and all transfers occur in a sealed environment that is in vapor equilibrium with the mother liquor. Here, a system is presented to retain crystals near the ejection point and away from the inaccessible dead volume at the bottom of the well by placing the crystals on a concave agarose pedestal (CAP) with the same chemical composition as the crystal mother liquor. The bowl-shaped CAP is impenetrable to crystals. Consequently, gravity will gently move the crystals into the optimal location for acoustic ejection. It is demonstrated that an agarose pedestal of this type is compatible with most commercially available crystallization conditions and that protein crystals are readily transferred from the agarose pedestal onto micromeshes with no loss in diffraction quality. It is also shown that crystals can be grown directly on CAPs, which avoids the need to transfer the crystals from the hanging drop to a CAP. This technology has been used to combine thermolysin and lysozyme crystals with an assortment of anomalously scattering heavy atoms. The results point towards a fast nanolitre method for crystal mounting and high-throughput screening.},

  doi          = {10.1107/S1399004714013728},

  journal      = {Acta Crystallographica. Section D: Biological Crystallography (Online)},

  number       = 1,

  volume       = 71,

  place        = {United States},

  year         = {Sun Jun 01 00:00:00 EDT 2014},

  month        = {Sun Jun 01 00:00:00 EDT 2014}

}

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Works referenced in this record:

Overview of the CCP 4 suite and current developments
journal, March 2011

Winn, Martyn D.; Ballard, Charles C.; Cowtan, Kevin D.
Acta Crystallographica Section D Biological Crystallography, Vol. 67, Issue 4
DOI: 10.1107/s0907444910045749

Approaches to automated protein crystal harvesting
journal, January 2014

Deller, Marc C.; Rupp, Bernhard
Acta Crystallographica Section F Structural Biology Communications, Vol. 70, Issue 2
DOI: 10.1107/s2053230x14000387

Quaternary Ammonium Oxidative Demethylation: X-ray Crystallographic, Resonance Raman, and UV–Visible Spectroscopic Analysis of a Rieske-Type Demethylase
journal, January 2012

Daughtry, Kelly D.; Xiao, Youli; Stoner-Ma, Deborah
Journal of the American Chemical Society, Vol. 134, Issue 5
DOI: 10.1021/ja2111898

Low Nanoliter Acoustic Transfer of Aqueous Fluids with High Precision and Accuracy of Volume Transfer and Positional Placement
journal, April 2008

Harris, D.
Journal of the Association for Laboratory Automation, Vol. 13, Issue 2
DOI: 10.1016/j.jala.2007.12.002

Binding of hydroxamic acid inhibitors to crystalline thermolysin suggests a pentacoordinate zinc intermediate in catalysis
journal, November 1981

Holmes, M. A.; Matthews, B. W.
Biochemistry, Vol. 20, Issue 24
DOI: 10.1021/bi00527a026

First experiences with semi-autonomous robotic harvesting of protein crystals
journal, March 2011

Viola, Robert; Walsh, Jace; Melka, Alex
Journal of Structural and Functional Genomics, Vol. 12, Issue 2
DOI: 10.1007/s10969-011-9103-5

Transfer of low nanoliter volumes between microplates using focused acoustics?automation considerations
journal, November 2003

Ellson, R.; Mutz, M.; Browning, B.
Journal of the Association for Laboratory Automation, Vol. 8, Issue 5
DOI: 10.1016/s1535-5535(03)00011-x

Coot model-building tools for molecular graphics
journal, November 2004

Emsley, Paul; Cowtan, Kevin
Acta Crystallographica Section D Biological Crystallography, Vol. 60, Issue 12, p. 2126-2132
DOI: 10.1107/S0907444904019158

Acoustically Mounted Microcrystals Yield High-Resolution X-ray Structures
journal, May 2011

Soares, Alexei S.; Engel, Matthew A.; Stearns, Richard
Biochemistry, Vol. 50, Issue 21
DOI: 10.1021/bi200549x

New directions in conventional methods of protein crystallization
journal, November 2009

Bolanos-Garcia, Victor M.; Chayen, Naomi E.
Progress in Biophysics and Molecular Biology, Vol. 101, Issue 1-3
DOI: 10.1016/j.pbiomolbio.2009.12.006

Automated Sample Mounting and Alignment System for Biological Crystallography at a Synchrotron Source
journal, April 2004

Snell, Gyorgy; Cork, Carl; Nordmeyer, Robert
Structure, Vol. 12, Issue 4
DOI: 10.1016/j.str.2004.03.011

Microcrystal manipulation with laser tweezers
journal, June 2013

Wagner, Armin; Duman, Ramona; Stevens, Bob
Acta Crystallographica Section D Biological Crystallography, Vol. 69, Issue 7
DOI: 10.1107/S090744491300958X

In-plate protein crystallization, in situ ligand soaking and X-ray diffraction
journal, August 2011

le Maire, Albane; Gelin, Muriel; Pochet, Sylvie
Acta Crystallographica Section D Biological Crystallography, Vol. 67, Issue 9
DOI: 10.1107/s0907444911023249

Overview of the CCP4 suite and current developments.
text, January 2011

Winn, Martyn D.; Ballard, Charles C.; Cowtan, Kevin D.
Apollo - University of Cambridge Repository
DOI: 10.17863/cam.52322

Bioactive Contaminants Leach from Disposable Laboratory Plasticware
journal, November 2008

McDonald, G. R.; Hudson, A. L.; Dunn, S. M. J.
Science, Vol. 322, Issue 5903
DOI: 10.1126/science.1162395

Acoustic matrix microseeding: improving protein crystal growth with minimal chemical bias
journal, April 2010

Villaseñor, Armando G.; Wong, April; Shao, Ada
Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 5
DOI: 10.1107/S0907444910005512

Nanolitre-scale crystallization using acoustic liquid-transfer technology
journal, July 2012

Villaseñor, Armando G.; Wong, April; Shao, Ada
Acta Crystallographica Section D Biological Crystallography, Vol. 68, Issue 8
DOI: 10.1107/S0907444912016617

Operator-assisted harvesting of protein crystals using a universal micromanipulation robot
journal, May 2007

Viola, Robert; Carman, Peter; Walsh, Jace
Journal of Applied Crystallography, Vol. 40, Issue 3
DOI: 10.1107/S0021889807012149

Acoustic methods for high-throughput protein crystal mounting at next-generation macromolecular crystallographic beamlines
journal, August 2013

Roessler, Christian G.; Kuczewski, Anthony; Stearns, Richard
Journal of Synchrotron Radiation, Vol. 20, Issue 5
DOI: 10.1107/s0909049513020372

CrystalDirect: a new method for automated crystal harvesting based on laser-induced photoablation of thin films
journal, September 2012

Cipriani, Florent; Röwer, Martin; Landret, Christophe
Acta Crystallographica Section D Biological Crystallography, Vol. 68, Issue 10
DOI: 10.1107/s0907444912031459

Binding of divalent copper ions to aspartic acid residue 52 in hen egg-white lysozyme
journal, August 1974

Teichberg, Vivian I.; Sharon, Nathan; Moult, John
Journal of Molecular Biology, Vol. 87, Issue 2
DOI: 10.1016/0022-2836(74)90155-7

First steps towards effective methods in exploiting high-throughput technologies for the determination of human protein structures of high biomedical value
journal, September 2006

Banci, L.; Bertini, I.; Cusack, S.
Acta Crystallographica Section D Biological Crystallography, Vol. 62, Issue 10
DOI: 10.1107/s0907444906029350

Crystal structure of photosystem II from Synechococcus elongatus at 3.8 Å resolution
journal, February 2001

Zouni, Athina; Witt, Horst-Tobias; Kern, Jan
Nature, Vol. 409, Issue 6821
DOI: 10.1038/35055589

Rapid sample delivery by acoustic injection for serial protein crystallography
journal, August 2014

Roessler, Christian; Allaire, Marc; Orville, Allen
Acta Crystallographica Section A Foundations and Advances, Vol. 70, Issue a1
DOI: 10.1107/s2053273314088433

ARP / wARP and molecular replacement
journal, September 2001

Perrakis, Anastassis; Harkiolaki, Maria; Wilson, Keith S.
Acta Crystallographica Section D Biological Crystallography, Vol. 57, Issue 10
DOI: 10.1107/s0907444901014007

Hitting the target: fragment screening with acoustic in situ co-crystallization of proteins plus fragment libraries on pin-mounted data-collection micromeshes
journal, April 2014

Yin, Xingyu; Scalia, Alexander; Leroy, Ludmila
Acta Crystallographica Section D Biological Crystallography, Vol. 70, Issue 5
DOI: 10.1107/s1399004713034603

Real-space refinement of the structure of hen egg-white lysozyme
journal, January 1974

Diamond, R.
Journal of Molecular Biology, Vol. 82, Issue 3
DOI: 10.1016/0022-2836(74)90598-1

Works referencing / citing this record:

Using sound pulses to solve the crystal-harvesting bottleneck
journal, October 2018

Samara, Yasmin N.; Brennan, Haley M.; McCarthy, Liam
Acta Crystallographica Section D Structural Biology, Vol. 74, Issue 10
DOI: 10.1107/s2059798318011506

Strategies for sample delivery for femtosecond crystallography
text, January 2019

Martiel, Isabelle; Müller-Werkmeister, Henrike M.; Cohen, Aina E.
International Union of Crystallography (IUCr)
DOI: 10.1107/s2059798318017953/ba5296sup1.pdf

Strategies for sample delivery for femtosecond crystallography
journal, February 2019

Martiel, Isabelle; Müller-Werkmeister, Henrike M.; Cohen, Aina E.
Acta Crystallographica Section D Structural Biology, Vol. 75, Issue 2
DOI: 10.1107/s2059798318017953

Gentle, fast and effective crystal soaking by acoustic dispensing
journal, March 2017

Collins, Patrick M.; Ng, Jia Tsing; Talon, Romain
Acta Crystallographica Section D Structural Biology, Vol. 73, Issue 3
DOI: 10.1107/s205979831700331x

A shared vision for macromolecular crystallography over the next five years
journal, November 2019

Förster, Andreas; Schulze-Briese, Clemens
Structural Dynamics, Vol. 6, Issue 6
DOI: 10.1063/1.5131017

Moving Liquids with Sound: The Physics of Acoustic Droplet Ejection for Robust Laboratory Automation in Life Sciences
journal, February 2016

Hadimioglu, Babur; Stearns, Richard; Ellson, Richard
Journal of Laboratory Automation, Vol. 21, Issue 1
DOI: 10.1177/2211068215615096

Focusing of Microcrystals and Liquid Condensates in Acoustofluidics
journal, February 2019

Gelin, Pierre; Van Lindt, Joris; Bratek-Skicki, Anna
Crystals, Vol. 9, Issue 3
DOI: 10.3390/cryst9030120

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Acoustic transfer of protein crystals from agarose pedestals to micromeshes for high-throughput screening

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Title: Acoustic transfer of protein crystals from agarose pedestals to micromeshes for high-throughput screening

Abstract

Citation Formats

Overview of the CCP 4 suite and current developments journal, March 2011

Approaches to automated protein crystal harvesting journal, January 2014

Quaternary Ammonium Oxidative Demethylation: X-ray Crystallographic, Resonance Raman, and UV–Visible Spectroscopic Analysis of a Rieske-Type Demethylase journal, January 2012

Low Nanoliter Acoustic Transfer of Aqueous Fluids with High Precision and Accuracy of Volume Transfer and Positional Placement journal, April 2008

Binding of hydroxamic acid inhibitors to crystalline thermolysin suggests a pentacoordinate zinc intermediate in catalysis journal, November 1981

First experiences with semi-autonomous robotic harvesting of protein crystals journal, March 2011

Transfer of low nanoliter volumes between microplates using focused acoustics?automation considerations journal, November 2003

Coot model-building tools for molecular graphics journal, November 2004

Acoustically Mounted Microcrystals Yield High-Resolution X-ray Structures journal, May 2011

New directions in conventional methods of protein crystallization journal, November 2009

Automated Sample Mounting and Alignment System for Biological Crystallography at a Synchrotron Source journal, April 2004

Microcrystal manipulation with laser tweezers journal, June 2013

In-plate protein crystallization, in situ ligand soaking and X-ray diffraction journal, August 2011

Overview of the CCP4 suite and current developments. text, January 2011

Bioactive Contaminants Leach from Disposable Laboratory Plasticware journal, November 2008

Acoustic matrix microseeding: improving protein crystal growth with minimal chemical bias journal, April 2010

Nanolitre-scale crystallization using acoustic liquid-transfer technology journal, July 2012

Operator-assisted harvesting of protein crystals using a universal micromanipulation robot journal, May 2007

Acoustic methods for high-throughput protein crystal mounting at next-generation macromolecular crystallographic beamlines journal, August 2013

CrystalDirect: a new method for automated crystal harvesting based on laser-induced photoablation of thin films journal, September 2012

Binding of divalent copper ions to aspartic acid residue 52 in hen egg-white lysozyme journal, August 1974

First steps towards effective methods in exploiting high-throughput technologies for the determination of human protein structures of high biomedical value journal, September 2006

Crystal structure of photosystem II from Synechococcus elongatus at 3.8 Å resolution journal, February 2001

Rapid sample delivery by acoustic injection for serial protein crystallography journal, August 2014

ARP / wARP and molecular replacement journal, September 2001

Hitting the target: fragment screening with acoustic in situ co-crystallization of proteins plus fragment libraries on pin-mounted data-collection micromeshes journal, April 2014

Real-space refinement of the structure of hen egg-white lysozyme journal, January 1974

Using sound pulses to solve the crystal-harvesting bottleneck journal, October 2018

Strategies for sample delivery for femtosecond crystallography text, January 2019

Strategies for sample delivery for femtosecond crystallography journal, February 2019

Gentle, fast and effective crystal soaking by acoustic dispensing journal, March 2017

A shared vision for macromolecular crystallography over the next five years journal, November 2019

Moving Liquids with Sound: The Physics of Acoustic Droplet Ejection for Robust Laboratory Automation in Life Sciences journal, February 2016

Focusing of Microcrystals and Liquid Condensates in Acoustofluidics journal, February 2019

Overview of the CCP 4 suite and current developments
journal, March 2011

Approaches to automated protein crystal harvesting
journal, January 2014

Quaternary Ammonium Oxidative Demethylation: X-ray Crystallographic, Resonance Raman, and UV–Visible Spectroscopic Analysis of a Rieske-Type Demethylase
journal, January 2012

Low Nanoliter Acoustic Transfer of Aqueous Fluids with High Precision and Accuracy of Volume Transfer and Positional Placement
journal, April 2008

Binding of hydroxamic acid inhibitors to crystalline thermolysin suggests a pentacoordinate zinc intermediate in catalysis
journal, November 1981

First experiences with semi-autonomous robotic harvesting of protein crystals
journal, March 2011

Transfer of low nanoliter volumes between microplates using focused acoustics?automation considerations
journal, November 2003

Coot model-building tools for molecular graphics
journal, November 2004

Acoustically Mounted Microcrystals Yield High-Resolution X-ray Structures
journal, May 2011

New directions in conventional methods of protein crystallization
journal, November 2009

Automated Sample Mounting and Alignment System for Biological Crystallography at a Synchrotron Source
journal, April 2004

Microcrystal manipulation with laser tweezers
journal, June 2013

In-plate protein crystallization, in situ ligand soaking and X-ray diffraction
journal, August 2011

Overview of the CCP4 suite and current developments.
text, January 2011

Bioactive Contaminants Leach from Disposable Laboratory Plasticware
journal, November 2008

Acoustic matrix microseeding: improving protein crystal growth with minimal chemical bias
journal, April 2010

Nanolitre-scale crystallization using acoustic liquid-transfer technology
journal, July 2012

Operator-assisted harvesting of protein crystals using a universal micromanipulation robot
journal, May 2007

Acoustic methods for high-throughput protein crystal mounting at next-generation macromolecular crystallographic beamlines
journal, August 2013

CrystalDirect: a new method for automated crystal harvesting based on laser-induced photoablation of thin films
journal, September 2012

Binding of divalent copper ions to aspartic acid residue 52 in hen egg-white lysozyme
journal, August 1974

First steps towards effective methods in exploiting high-throughput technologies for the determination of human protein structures of high biomedical value
journal, September 2006

Crystal structure of photosystem II from Synechococcus elongatus at 3.8 Å resolution
journal, February 2001

Rapid sample delivery by acoustic injection for serial protein crystallography
journal, August 2014

ARP / wARP and molecular replacement
journal, September 2001

Hitting the target: fragment screening with acoustic in situ co-crystallization of proteins plus fragment libraries on pin-mounted data-collection micromeshes
journal, April 2014

Real-space refinement of the structure of hen egg-white lysozyme
journal, January 1974

Using sound pulses to solve the crystal-harvesting bottleneck
journal, October 2018

Strategies for sample delivery for femtosecond crystallography
text, January 2019

Strategies for sample delivery for femtosecond crystallography
journal, February 2019

Gentle, fast and effective crystal soaking by acoustic dispensing
journal, March 2017

A shared vision for macromolecular crystallography over the next five years
journal, November 2019

Moving Liquids with Sound: The Physics of Acoustic Droplet Ejection for Robust Laboratory Automation in Life Sciences
journal, February 2016

Focusing of Microcrystals and Liquid Condensates in Acoustofluidics
journal, February 2019