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Title: Crystallization of lysozyme with (R)-, (S)- and (RS)-2-methyl-2,4-pentanediol

Abstract

Chiral control of crystallization has ample precedent in the small-molecule world, but relatively little is known about the role of chirality in protein crystallization. In this study, lysozyme was crystallized in the presence of the chiral additive 2-methyl-2,4-pentanediol (MPD) separately using the R and S enantiomers as well as with a racemic RS mixture. Crystals grown with (R)-MPD had the most order and produced the highest resolution protein structures. This result is consistent with the observation that in the crystals grown with (R)-MPD and (RS)-MPD the crystal contacts are made by (R)-MPD, demonstrating that there is preferential interaction between lysozyme and this enantiomer. These findings suggest that chiral interactions are important in protein crystallization.

Authors:
 [1];  [2];  [3];  [4];  [5];  [5];  [5];  [6];  [5]
  1. Yeshiva Univ., New York, NY (United States); Stanford Univ., Stanford, CA (United States)
  2. Brookhaven National Lab. (BNL), Upton, NY (United States)
  3. Yeshiva Univ., New York, NY (United States); Univ. of Pennsylvania, Philadelphia, PA (United States)
  4. Yeshiva Univ., New York, NY (United States); Yeshiva Univ., Bronx, NY (United States)
  5. Yeshiva Univ., New York, NY (United States)
  6. Collegiate School, New York, NY (United States)
Publication Date:
Research Org.:
Brookhaven National Laboratory (BNL), Upton, NY (United States)
Sponsoring Org.:
USDOE Office of Science (SC)
OSTI Identifier:
1193215
Report Number(s):
BNL-108006-2015-JA
Journal ID: ISSN 1399-0047; ABCRE6; R&D Project: LS001
Grant/Contract Number:  
SC00112704
Resource Type:
Accepted Manuscript
Journal Name:
Acta Crystallographica. Section D: Biological Crystallography (Online)
Additional Journal Information:
Journal Name: Acta Crystallographica. Section D: Biological Crystallography (Online); Journal Volume: 71; Journal Issue: 3; Journal ID: ISSN 1399-0047
Publisher:
International Union of Crystallography
Country of Publication:
United States
Language:
English
Subject:
36 MATERIALS SCIENCE; MPD; crystallization additive; precipitant; high-resolution protein structures; chirality

Citation Formats

Stauber, Mark, Jakoncic, Jean, Berger, Jacob, Karp, Jerome M., Axelbaum, Ariel, Sastow, Dahniel, Buldyrev, Sergey V., Hrnjez, Bruce J., and Asherie, Neer. Crystallization of lysozyme with (R)-, (S)- and (RS)-2-methyl-2,4-pentanediol. United States: N. p., 2015. Web. doi:10.1107/S1399004714025061.
Stauber, Mark, Jakoncic, Jean, Berger, Jacob, Karp, Jerome M., Axelbaum, Ariel, Sastow, Dahniel, Buldyrev, Sergey V., Hrnjez, Bruce J., & Asherie, Neer. Crystallization of lysozyme with (R)-, (S)- and (RS)-2-methyl-2,4-pentanediol. United States. https://doi.org/10.1107/S1399004714025061
Stauber, Mark, Jakoncic, Jean, Berger, Jacob, Karp, Jerome M., Axelbaum, Ariel, Sastow, Dahniel, Buldyrev, Sergey V., Hrnjez, Bruce J., and Asherie, Neer. Sun . "Crystallization of lysozyme with (R)-, (S)- and (RS)-2-methyl-2,4-pentanediol". United States. https://doi.org/10.1107/S1399004714025061. https://www.osti.gov/servlets/purl/1193215.
@article{osti_1193215,
title = {Crystallization of lysozyme with (R)-, (S)- and (RS)-2-methyl-2,4-pentanediol},
author = {Stauber, Mark and Jakoncic, Jean and Berger, Jacob and Karp, Jerome M. and Axelbaum, Ariel and Sastow, Dahniel and Buldyrev, Sergey V. and Hrnjez, Bruce J. and Asherie, Neer},
abstractNote = {Chiral control of crystallization has ample precedent in the small-molecule world, but relatively little is known about the role of chirality in protein crystallization. In this study, lysozyme was crystallized in the presence of the chiral additive 2-methyl-2,4-pentanediol (MPD) separately using the R and S enantiomers as well as with a racemic RS mixture. Crystals grown with (R)-MPD had the most order and produced the highest resolution protein structures. This result is consistent with the observation that in the crystals grown with (R)-MPD and (RS)-MPD the crystal contacts are made by (R)-MPD, demonstrating that there is preferential interaction between lysozyme and this enantiomer. These findings suggest that chiral interactions are important in protein crystallization.},
doi = {10.1107/S1399004714025061},
journal = {Acta Crystallographica. Section D: Biological Crystallography (Online)},
number = 3,
volume = 71,
place = {United States},
year = {Sun Mar 01 00:00:00 EST 2015},
month = {Sun Mar 01 00:00:00 EST 2015}
}

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