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Title: Image analysis of anatomical traits in stalk transections of maize and other grasses

Abstract

Grass stalks architecturally support leaves and reproductive structures, functionally support the transport of water and nutrients, and are harvested for multiple agricultural uses. Research on these basic and applied aspects of grass stalks would benefit from improved capabilities for measuring internal anatomical features. In particular, methods suitable for phenotyping populations of plants are needed.

Authors:
 [1];  [2];  [2];  [2];  [1]
  1. Univ. of Wisconsin, Madison, WI (United States). Dept. of Botany
  2. Univ. of Wisconsin, Madison, WI (United States). Dept. of Agronomy; DOE Great Lakes Bioenergy Research Center (GLBRC), Madison, WI (United States)
Publication Date:
Research Org.:
Great Lakes Bioenergy Research Center (GLBRC), Madison, WI (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1184805
Grant/Contract Number:  
FC02-07ER64494
Resource Type:
Accepted Manuscript
Journal Name:
Plant Methods
Additional Journal Information:
Journal Volume: 11; Journal Issue: 1; Journal ID: ISSN 1746-4811
Publisher:
BioMed Central
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; 09 BIOMASS FUELS

Citation Formats

Heckwolf, Sven, Heckwolf, Marlies, Kaeppler, Shawn M., de Leon, Natalia, and Spalding, Edgar P. Image analysis of anatomical traits in stalk transections of maize and other grasses. United States: N. p., 2015. Web. doi:10.1186/s13007-015-0070-x.
Heckwolf, Sven, Heckwolf, Marlies, Kaeppler, Shawn M., de Leon, Natalia, & Spalding, Edgar P. Image analysis of anatomical traits in stalk transections of maize and other grasses. United States. https://doi.org/10.1186/s13007-015-0070-x
Heckwolf, Sven, Heckwolf, Marlies, Kaeppler, Shawn M., de Leon, Natalia, and Spalding, Edgar P. Thu . "Image analysis of anatomical traits in stalk transections of maize and other grasses". United States. https://doi.org/10.1186/s13007-015-0070-x. https://www.osti.gov/servlets/purl/1184805.
@article{osti_1184805,
title = {Image analysis of anatomical traits in stalk transections of maize and other grasses},
author = {Heckwolf, Sven and Heckwolf, Marlies and Kaeppler, Shawn M. and de Leon, Natalia and Spalding, Edgar P},
abstractNote = {Grass stalks architecturally support leaves and reproductive structures, functionally support the transport of water and nutrients, and are harvested for multiple agricultural uses. Research on these basic and applied aspects of grass stalks would benefit from improved capabilities for measuring internal anatomical features. In particular, methods suitable for phenotyping populations of plants are needed.},
doi = {10.1186/s13007-015-0070-x},
journal = {Plant Methods},
number = 1,
volume = 11,
place = {United States},
year = {Thu Apr 09 00:00:00 EDT 2015},
month = {Thu Apr 09 00:00:00 EDT 2015}
}

Journal Article:
Free Publicly Available Full Text
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Citation Metrics:
Cited by: 28 works
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Figures / Tables:

Figure 1 Figure 1: The initial processing steps of maize stalk transections. A) Original, unprocessed images of four stalk transections. The two on the left are the same genotype. The two on the right are the same genotype but different from those on the left. B) Segmenting all non-background objects by amore » simple thresholding technique identifies the stalk samples and smaller non-stalk objects such as scratches and debris. C) A filtering step based on object size and shape results in a binary image consisting of background and stalk samples. D) A crop box centered on each of the stalk objects is placed on the original image to allow the user to make adjustments to the scenes to be processed.« less

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Works referenced in this record:

Structural development and stability of rice Oryza sativa L. var. Nerica 1
journal, August 2006


Modifying crops to increase cell wall digestibility
journal, April 2012


An automatic method for robust and fast cell detection in bright field images from high-throughput microscopy
text, January 2013


Computing 2-D min, median, and max filters
journal, May 1993

  • Gil, J.; Werman, M.
  • IEEE Transactions on Pattern Analysis and Machine Intelligence, Vol. 15, Issue 5
  • DOI: 10.1109/34.211471

Best practices for biofuels
journal, June 2014


Survey over image thresholding techniques and quantitative performance evaluation
journal, January 2004

  • Sankur, Bu¨lent
  • Journal of Electronic Imaging, Vol. 13, Issue 1
  • DOI: 10.1117/1.1631315

A high-throughput imaging auxanometer for roots and hypocotyls of Arabidopsis using a 2D skeletonizing algorithm
journal, April 2014

  • Fraas, Simon; Niehoff, Vera; Lüthen, Hartwig
  • Physiologia Plantarum, Vol. 151, Issue 1
  • DOI: 10.1111/ppl.12183

Chemical composition and cell wall polysaccharide degradability of pith and rind tissues from mature maize internodes
journal, March 2012


A Mathematical Model of Water and Nutrient Transport in Xylem Vessels of a Wheat Plant
journal, February 2014


Divergent selection for ester-linked diferulates in maize pith stalk tissues. Effects on cell wall composition and degradability
journal, November 2012


Maize Stem Tissues
journal, January 2006


Vessel diameter-stem diameter scaling across woody angiosperms and the ecological causes of xylem vessel diameter variation
journal, December 2012

  • Olson, Mark E.; Rosell, Julieta A.
  • New Phytologist, Vol. 197, Issue 4
  • DOI: 10.1111/nph.12097

Pressure and Flow Relations in Vascular Bundles of the Tomato Plant
journal, January 1966


Pre-processing, registration and selection of adaptive optics corrected retinal images
journal, April 2013

  • Ramaswamy, Gomathy; Devaney, Nicholas
  • Ophthalmic and Physiological Optics, Vol. 33, Issue 4
  • DOI: 10.1111/opo.12068

Maize stem tissues: cell wall concentration and composition during development
journal, November 2009


Influence of cell size and cell wall Volume Fraction on Failure Properties of Potato and Carrot Tissue
journal, February 2005


Efficient Non-Maximum Suppression
conference, January 2006

  • Neubeck, A.; Van Gool, L.
  • 18th International Conference on Pattern Recognition (ICPR'06)
  • DOI: 10.1109/ICPR.2006.479

Extraction of target specimens from bioholographic images using interactive graph cuts
journal, December 2013


Stem and root characteristics associated with lodging resistance in four winter wheat cultivars
journal, October 1994


Macro-vision and grey level granulometry for quantification of tomato pericarp structure
journal, February 2008


In Search of a Maize Ideotype for Cell Wall Enzymatic Degradability Using Histological and Biochemical Lignin Characterization
journal, July 2005

  • Méchin, Valérie; Argillier, Odile; Rocher, Françoise
  • Journal of Agricultural and Food Chemistry, Vol. 53, Issue 15
  • DOI: 10.1021/jf050722f

Statistical Mapping of Maize Bundle Intensity at the Stem Scale Using Spatial Normalisation of Replicated Images
journal, March 2014


On the Strength of Herbaceous Vascular Plant Stems
journal, July 1997


Genetic Improvement of Plants for Enhanced Bio-Ethanol Production
journal, February 2013


Mathematical modelling of the cellular mechanics of plants
journal, July 2003

  • Bruce, David M.
  • Philosophical Transactions of the Royal Society of London. Series B: Biological Sciences, Vol. 358, Issue 1437
  • DOI: 10.1098/rstb.2003.1337

Genes controlling plant architecture
journal, April 2006


An automatic method for robust and fast cell detection in bright field images from high-throughput microscopy
journal, January 2013

  • Buggenthin, Felix; Marr, Carsten; Schwarzfischer, Michael
  • BMC Bioinformatics, Vol. 14, Issue 1
  • DOI: 10.1186/1471-2105-14-297

Color Quantification of Stained Maize Stem Section Describes Lignin Spatial Distribution within the Whole Stem
journal, March 2013

  • Zhang, Yu; Legay, Sylvain; Barrière, Yves
  • Journal of Agricultural and Food Chemistry, Vol. 61, Issue 13
  • DOI: 10.1021/jf400912s

On the strength, stiffness and stability of tubular plant stems and leaves
journal, April 1992


Genetic Diversity of a Maize Association Population with Restricted Phenology
journal, January 2011


A Mathematical Model of Water and Nutrient Transport in Xylem Vessels of a Wheat Plant
journal, February 2014


Chemical composition and cell wall polysaccharide degradability of pith and rind tissues from mature maize internodes
journal, March 2012


Genes controlling plant architecture
journal, April 2006


Divergent selection for ester-linked diferulates in maize pith stalk tissues. Effects on cell wall composition and degradability
journal, November 2012


Modifying crops to increase cell wall digestibility
journal, April 2012


Macro-vision and grey level granulometry for quantification of tomato pericarp structure
journal, February 2008


Butterworth equations for homomorphic filtering of images
journal, June 1998


In Search of a Maize Ideotype for Cell Wall Enzymatic Degradability Using Histological and Biochemical Lignin Characterization
journal, July 2005

  • Méchin, Valérie; Argillier, Odile; Rocher, Françoise
  • Journal of Agricultural and Food Chemistry, Vol. 53, Issue 15
  • DOI: 10.1021/jf050722f

Color Quantification of Stained Maize Stem Section Describes Lignin Spatial Distribution within the Whole Stem
journal, March 2013

  • Zhang, Yu; Legay, Sylvain; Barrière, Yves
  • Journal of Agricultural and Food Chemistry, Vol. 61, Issue 13
  • DOI: 10.1021/jf400912s

Structural development and stability of rice Oryza sativa L. var. Nerica 1
journal, August 2006


Mathematical modelling of the cellular mechanics of plants
journal, July 2003

  • Bruce, David M.
  • Philosophical Transactions of the Royal Society of London. Series B: Biological Sciences, Vol. 358, Issue 1437
  • DOI: 10.1098/rstb.2003.1337

Influence of cell size and cell wall Volume Fraction on Failure Properties of Potato and Carrot Tissue
journal, February 2005


Vessel diameter-stem diameter scaling across woody angiosperms and the ecological causes of xylem vessel diameter variation
journal, December 2012

  • Olson, Mark E.; Rosell, Julieta A.
  • New Phytologist, Vol. 197, Issue 4
  • DOI: 10.1111/nph.12097

Pre-processing, registration and selection of adaptive optics corrected retinal images
journal, April 2013

  • Ramaswamy, Gomathy; Devaney, Nicholas
  • Ophthalmic and Physiological Optics, Vol. 33, Issue 4
  • DOI: 10.1111/opo.12068

A high-throughput imaging auxanometer for roots and hypocotyls of Arabidopsis using a 2D skeletonizing algorithm
journal, April 2014

  • Fraas, Simon; Niehoff, Vera; Lüthen, Hartwig
  • Physiologia Plantarum, Vol. 151, Issue 1
  • DOI: 10.1111/ppl.12183

Survey over image thresholding techniques and quantitative performance evaluation
journal, January 2004

  • Sankur, Bu¨lent
  • Journal of Electronic Imaging, Vol. 13, Issue 1
  • DOI: 10.1117/1.1631315

Best practices for biofuels
journal, June 2014


Statistical Mapping of Maize Bundle Intensity at the Stem Scale Using Spatial Normalisation of Replicated Images
journal, March 2014


Genetic Diversity of a Maize Association Population with Restricted Phenology
journal, January 2011


Genetic Improvement of Plants for Enhanced Bio-Ethanol Production
journal, February 2013


Using Flatbed Scanners to Collect High-resolution Time-lapsed Images of the Arabidopsis Root Gravitropic Response
journal, January 2014

  • Smith, Halie C.; Niewohner, Devon J.; Dewey, Grant D.
  • Journal of Visualized Experiments, Issue 83
  • DOI: 10.3791/50878-v

An automatic method for robust and fast cell detection in bright field images from high-throughput microscopy
text, January 2013


Works referencing / citing this record:

Plasticity of Sorghum Stem Biomass Accumulation in Response to Water Deficit: A Multiscale Analysis from Internode Tissue to Plant Level
journal, September 2017


PlantSize Offers an Affordable, Non-destructive Method to Measure Plant Size and Color in Vitro
journal, February 2018


A novel rind puncture technique to measure rind thickness and diameter in plant stalks
journal, April 2020


Measuring the compressive modulus of elasticity of pith-filled plant stems
journal, November 2017


A Novel Rind Puncture Technique to Measure Rind Thickness and Diameter in Plant Stalks
posted_content, April 2020

  • Seegmiller, Will H.; Graves, Jadzia; Robertson, Daniel J.
  • Research Square [preprints]
  • DOI: 10.21203/rs.2.19825/v1

A Novel Rind Puncture Technique to Measure Rind Thickness and Diameter in Plant Stalks
preprint, April 2020


Genome-wide association analysis of stalk biomass and anatomical traits in maize
journal, January 2019

  • Mazaheri, Mona; Heckwolf, Marlies; Vaillancourt, Brieanne
  • BMC Plant Biology, Vol. 19, Issue 1
  • DOI: 10.1186/s12870-019-1653-x

TIPS: a system for automated image-based phenotyping of maize tassels
journal, March 2017


Crop Phenomics: Current Status and Perspectives
journal, June 2019


Phenotyping analysis of maize stem using micro-computed tomography at the elongation and tasseling stages
journal, January 2020


Genome-wide association analysis of stalk biomass and anatomical traits in maize
journal, January 2019

  • Mazaheri, Mona; Heckwolf, Marlies; Vaillancourt, Brieanne
  • BMC Plant Biology, Vol. 19, Issue 1
  • DOI: 10.1186/s12870-019-1653-x

TIPS: a system for automated image-based phenotyping of maize tassels
journal, March 2017


Histological quantification of maize stem sections from FASGA-stained images
journal, November 2017


Measuring the compressive modulus of elasticity of pith-filled plant stems
journal, November 2017


The elastic modulus for maize stems
journal, February 2018


Phenotyping analysis of maize stem using micro-computed tomography at the elongation and tasseling stages
journal, January 2020


A novel rind puncture technique to measure rind thickness and diameter in plant stalks
journal, April 2020


High-throughput micro-phenotyping measurements applied to assess stalk lodging in maize (Zea mays L.)
journal, October 2018


Crop Phenomics: Current Status and Perspectives
journal, June 2019