Metastatic Melanoma Induced Metabolic Changes in C57BL/6J Mouse Stomach Measured by 1H NMR Spectroscopy

Hu, M; Wang, Xiliang

doi:10.4172/2153-0769.1000135

Title: Metastatic Melanoma Induced Metabolic Changes in C57BL/6J Mouse Stomach Measured by 1H NMR Spectroscopy

Abstract

Melanoma is a malignant tumor of melanocytes with high capability of invasion and rapid metastasis to other organs. Malignant melanoma is the most common metastatic malignancy found in gastrointestinal tract (GI). To the best of our knowledge, previous studies of melanoma in gastrointestinal tract are all clinical case reports. In this work, 1H NMR-based metabolomics approach is used to investigate the metabolite profiles differences of stomach tissue extracts of metastatic B16-F10 melanoma in C57BL/6J mouse and search for specific metabolite biomarker candidates. Principal Component Analysis (PCA), an unsupervised multivariate data analysis method, is used to detect possible outliers, while Orthogonal Projection to Latent Structure (OPLS), a supervised multivariate data analysis method, is employed to evaluate important metabolites responsible for discriminating the control and the melanoma groups. Both PCA and OPLS results reveal that the melanoma group can be well separated from its control group. Among the 50 identified metabolites, it is found that the concentrations of 19 metabolites are statistically and significantly changed with the levels of O-phosphocholine and hypoxanthine down-regulated while the levels of isoleucine, leucine, valine, isobutyrate, threonine, cadaverine, alanine, glutamate, glutamine, methionine, citrate, asparagine, tryptophan, glycine, serine, uracil, and formate up-regulated in the melanoma group. These significantlymore »« less

Authors:

Hu, M; Wang, Xiliang ^[1]

Pacific Northwest National Lab. (PNNL), Richland, WA (United States)

Publication Date:: Fri Dec 05 00:00:00 EST 2014

Research Org.:: Pacific Northwest National Laboratory (PNNL), Richland, WA (United States). Environmental Molecular Sciences Laboratory (EMSL)

Sponsoring Org.:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

OSTI Identifier:: 1171305

Report Number(s):: PNNL-SA-105912
Journal ID: ISSN 2153-0769; 17497b; 400412000

Grant/Contract Number:: AC05-76RL01830

Resource Type:: Accepted Manuscript

Journal Name:: Metabolomics

Additional Journal Information:: Journal Volume: 04; Journal Issue: 02; Journal ID: ISSN 2153-0769

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES; NMR metabolomics; B16-F10 melanoma; stomach; metabolomics; multivariate analysis; OPLS; and PCA; Environmental Molecular Sciences Laboratory

Citation Formats


                    Hu, M, and Wang, Xiliang. Metastatic Melanoma Induced Metabolic Changes in C57BL/6J Mouse Stomach Measured by 1H NMR Spectroscopy.  United States: N. p., 2014. 
Web.  doi:10.4172/2153-0769.1000135.

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                    Hu, M, & Wang, Xiliang. Metastatic Melanoma Induced Metabolic Changes in C57BL/6J Mouse Stomach Measured by 1H NMR Spectroscopy.  United States.  https://doi.org/10.4172/2153-0769.1000135

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                    Hu, M, and Wang, Xiliang. Fri .  
"Metastatic Melanoma Induced Metabolic Changes in C57BL/6J Mouse Stomach Measured by 1H NMR Spectroscopy".  United States.  https://doi.org/10.4172/2153-0769.1000135.  https://www.osti.gov/servlets/purl/1171305.

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@article{osti_1171305,

  title        = {Metastatic Melanoma Induced Metabolic Changes in C57BL/6J Mouse Stomach Measured by 1H NMR Spectroscopy},

  author       = {Hu, M and Wang, Xiliang},

  abstractNote = {Melanoma is a malignant tumor of melanocytes with high capability of invasion and rapid metastasis to other organs. Malignant melanoma is the most common metastatic malignancy found in gastrointestinal tract (GI). To the best of our knowledge, previous studies of melanoma in gastrointestinal tract are all clinical case reports. In this work, 1H NMR-based metabolomics approach is used to investigate the metabolite profiles differences of stomach tissue extracts of metastatic B16-F10 melanoma in C57BL/6J mouse and search for specific metabolite biomarker candidates. Principal Component Analysis (PCA), an unsupervised multivariate data analysis method, is used to detect possible outliers, while Orthogonal Projection to Latent Structure (OPLS), a supervised multivariate data analysis method, is employed to evaluate important metabolites responsible for discriminating the control and the melanoma groups. Both PCA and OPLS results reveal that the melanoma group can be well separated from its control group. Among the 50 identified metabolites, it is found that the concentrations of 19 metabolites are statistically and significantly changed with the levels of O-phosphocholine and hypoxanthine down-regulated while the levels of isoleucine, leucine, valine, isobutyrate, threonine, cadaverine, alanine, glutamate, glutamine, methionine, citrate, asparagine, tryptophan, glycine, serine, uracil, and formate up-regulated in the melanoma group. These significantly changed metabolites are associated with multiple biological pathways and may be potential biomarkers for metastatic melanoma in stomach.},

  doi          = {10.4172/2153-0769.1000135},

  journal      = {Metabolomics},

  number       = 02,

  volume       = 04,

  place        = {United States},

  year         = {Fri Dec 05 00:00:00 EST 2014},

  month        = {Fri Dec 05 00:00:00 EST 2014}

}

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Works referencing / citing this record:

Discovery of potential biomarkers in human melanoma cells with different metastatic potential by metabolic and lipidomic profiling
journal, August 2017

Kim, Hye-Youn; Lee, Hwanhui; Kim, So-Hyun
Scientific Reports, Vol. 7, Issue 1
DOI: 10.1038/s41598-017-08433-9

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Title: Metastatic Melanoma Induced Metabolic Changes in C57BL/6J Mouse Stomach Measured by 1H NMR Spectroscopy

Abstract

Citation Formats

Discovery of potential biomarkers in human melanoma cells with different metastatic potential by metabolic and lipidomic profiling journal, August 2017

Discovery of potential biomarkers in human melanoma cells with different metastatic potential by metabolic and lipidomic profiling
journal, August 2017