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Title: High temperature pre-digestion of corn stover biomass for improved product yields

Abstract

Introduction: The efficient conversion of lignocellulosic feedstocks remains a key step in the commercialization of biofuels. One of the barriers to cost-effective conversion of lignocellulosic biomass to sugars remains the enzymatic saccharification process step. Here, we describe a novel hybrid processing approach comprising enzymatic pre-digestion with newly characterized hyperthermophilic enzyme cocktails followed by conventional saccharification with commercial enzyme preparations. Dilute acid pretreated corn stover was subjected to this new procedure to test its efficacy. Thermal tolerant enzymes from Acidothermus cellulolyticus and Caldicellulosiruptor bescii were used to pre-digest pretreated biomass at elevated temperatures prior to saccharification by the commercial cellulase formulation. Results: We report that pre-digestion of biomass with these enzymes at elevated temperatures prior to addition of the commercial cellulase formulation increased conversion rates and yields when compared to commercial cellulase formulation alone under low solids conditions. In conclusion, Our results demonstrating improvements in rates and yields of conversion point the way forward for hybrid biomass conversion schemes utilizing catalytic amounts of hyperthermophilic enzymes.

Authors:
 [1];  [1];  [1];  [2];  [2];  [1];  [1]
+ Show Author Affiliations
  1. National Renewable Energy Lab. (NREL), Golden, CO (United States). Chemical Biosciences Center.
  2. National Renewable Energy Lab. (NREL), Golden, CO (United States). National Bioenergy Center.
Publication Date:
Research Org.:
National Renewable Energy Laboratory (NREL), Golden, CO (United States)
Sponsoring Org.:
USDOE Office of Energy Efficiency and Renewable Energy (EERE), Sustainable Transportation Office. Bioenergy Technologies Office (BETO)
OSTI Identifier:
1166653
Report Number(s):
NREL/JA-2700-62534
Journal ID: ISSN 1754-6834; MainId:24397;UUID:f6a9aa36-d51c-e411-b769-d89d67132a6d;MainAdminID:12873
Grant/Contract Number:  
AC36-08GO28308
Resource Type:
Accepted Manuscript
Journal Name:
Biotechnology for Biofuels
Additional Journal Information:
Journal Volume: 7; Journal Issue: 1; Journal ID: ISSN 1754-6834
Publisher:
BioMed Central
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; 59 BASIC BIOLOGICAL SCIENCES; biomass; pretreatment; enzymatic hydrolysis; CelA; E1; Caldicellulosiruptor bescii; Acidothermus cellulolyticus; Thermotoga maritima

Citation Formats

Brunecky, Roman, Hobdey, Sarah E., Taylor, Larry E., Tao, Ling, Tucker, Melvin P., Himmel, Michael E., and Decker, Stephen R. High temperature pre-digestion of corn stover biomass for improved product yields. United States: N. p., 2014. Web. doi:10.1186/s13068-014-0170-2.
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Brunecky, Roman, Hobdey, Sarah E., Taylor, Larry E., Tao, Ling, Tucker, Melvin P., Himmel, Michael E., & Decker, Stephen R. High temperature pre-digestion of corn stover biomass for improved product yields. United States. https://doi.org/10.1186/s13068-014-0170-2
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Brunecky, Roman, Hobdey, Sarah E., Taylor, Larry E., Tao, Ling, Tucker, Melvin P., Himmel, Michael E., and Decker, Stephen R. Wed . "High temperature pre-digestion of corn stover biomass for improved product yields". United States. https://doi.org/10.1186/s13068-014-0170-2. https://www.osti.gov/servlets/purl/1166653.
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@article{osti_1166653,
title = {High temperature pre-digestion of corn stover biomass for improved product yields},
author = {Brunecky, Roman and Hobdey, Sarah E. and Taylor, Larry E. and Tao, Ling and Tucker, Melvin P. and Himmel, Michael E. and Decker, Stephen R.},
abstractNote = {Introduction: The efficient conversion of lignocellulosic feedstocks remains a key step in the commercialization of biofuels. One of the barriers to cost-effective conversion of lignocellulosic biomass to sugars remains the enzymatic saccharification process step. Here, we describe a novel hybrid processing approach comprising enzymatic pre-digestion with newly characterized hyperthermophilic enzyme cocktails followed by conventional saccharification with commercial enzyme preparations. Dilute acid pretreated corn stover was subjected to this new procedure to test its efficacy. Thermal tolerant enzymes from Acidothermus cellulolyticus and Caldicellulosiruptor bescii were used to pre-digest pretreated biomass at elevated temperatures prior to saccharification by the commercial cellulase formulation. Results: We report that pre-digestion of biomass with these enzymes at elevated temperatures prior to addition of the commercial cellulase formulation increased conversion rates and yields when compared to commercial cellulase formulation alone under low solids conditions. In conclusion, Our results demonstrating improvements in rates and yields of conversion point the way forward for hybrid biomass conversion schemes utilizing catalytic amounts of hyperthermophilic enzymes.},
doi = {10.1186/s13068-014-0170-2},
journal = {Biotechnology for Biofuels},
number = 1,
volume = 7,
place = {United States},
year = {Wed Dec 03 00:00:00 EST 2014},
month = {Wed Dec 03 00:00:00 EST 2014}
}
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https://doi.org/10.1186/s13068-014-0170-2
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Works referenced in this record:

The challenge of enzyme cost in the production of lignocellulosic biofuels
journal, November 2011

  • Klein-Marcuschamer, Daniel; Oleskowicz-Popiel, Piotr; Simmons, Blake A.
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Modelling ethanol production from cellulose: separate hydrolysis and fermentation versus simultaneous saccharification and fermentation
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A short review on SSF – an interesting process option for ethanol production from lignocellulosic feedstocks
journal, January 2008

  • Olofsson, Kim; Bertilsson, Magnus; Lidén, Gunnar
  • Biotechnology for Biofuels, Vol. 1, Issue 1
  • DOI: 10.1186/1754-6834-1-7

Pretreatment Methods for Bioethanol Production
journal, June 2014

Comparison of SHF and SSF processes for the bioconversion of steam-exploded wheat straw
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  • Alfani, F.; Gallifuoco, A.; Saporosi, A.
  • Journal of Industrial Microbiology and Biotechnology, Vol. 25, Issue 4, p. 184-192
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Revealing Nature's Cellulase Diversity The Digestion Mechanism of Caldicellulosiruptor bescii CelA
journal, December 2013

A new thermostable endoglucanase,Acidothermus cellulolyticus E1: Synergism withTrichoderma reesei CBH I and comparison to Thermomonospora fusca E5
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Crystal Structure of Thermostable Family 5 Endocellulase E1 from Acidothermus cellulolyticus in Complex with Cellotetraose † , ‡
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Purification and properties of recombinant ?-glucosidase of the hyperthermophilic bacterium Thermotoga maritima
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Substrate specificity and transglycosylation catalyzed by a thermostable β-glucosidase from marine hyperthermophile Thermotoga neapolitana
journal, August 2005

  • Park, Tak-Hyun; Choi, Ki-Won; Park, Cheon-Seok
  • Applied Microbiology and Biotechnology, Vol. 69, Issue 4
  • DOI: 10.1007/s00253-005-0055-1

Substrate specificity and transglycosylation catalyzed by a thermostable β-glucosidase from marine hyperthermophile Thermotoga neapolitana
journal, August 2005

  • Park, Tak-Hyun; Choi, Ki-Won; Park, Cheon-Seok
  • Applied Microbiology and Biotechnology, Vol. 69, Issue 4
  • DOI: 10.1007/s00253-005-0055-1

New thermostable endoglucanase from Spirochaeta thermophila and its mutants with altered substrate preferences
journal, January 2021

  • Hämäläinen, Veera; Barajas-López, Juan De Dios; Berlina, Yana
  • Applied Microbiology and Biotechnology, Vol. 105, Issue 3
  • DOI: 10.1007/s00253-020-11077-x

Crystal Structure of Thermostable Family 5 Endocellulase E1 from Acidothermus cellulolyticus in Complex with Cellotetraose † , ‡
journal, January 1996

  • Sakon, Joshua; Adney, William S.; Himmel, Michael E.
  • Biochemistry, Vol. 35, Issue 33
  • DOI: 10.1021/bi9604439

Comparison of SHF and SSF processes for the bioconversion of steam-exploded wheat straw
journal, October 2000

  • Alfani, F.; Gallifuoco, A.; Saporosi, A.
  • Journal of Industrial Microbiology and Biotechnology, Vol. 25, Issue 4, p. 184-192
  • DOI: 10.1038/sj.jim.7000054

Modelling ethanol production from cellulose: separate hydrolysis and fermentation versus simultaneous saccharification and fermentation
journal, January 2009

  • Drissen, R. E. T.; Maas, R. H. W.; Tramper, J.
  • Biocatalysis and Biotransformation, Vol. 27, Issue 1
  • DOI: 10.1080/10242420802564358

Revealing Nature's Cellulase Diversity The Digestion Mechanism of Caldicellulosiruptor bescii CelA
journal, December 2013

A short review on SSF – an interesting process option for ethanol production from lignocellulosic feedstocks
journal, January 2008

  • Olofsson, Kim; Bertilsson, Magnus; Lidén, Gunnar
  • Biotechnology for Biofuels, Vol. 1, Issue 1
  • DOI: 10.1186/1754-6834-1-7
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    Works referencing / citing this record:

    Galactomannan degradation by thermophilic enzymes: a hot topic for biotechnological applications
    journal, January 2019

    • Aulitto, Martina; Fusco, Salvatore; Limauro, Danila
    • World Journal of Microbiology and Biotechnology, Vol. 35, Issue 2
    • DOI: 10.1007/s11274-019-2591-3

    Galactomannan degradation by thermophilic enzymes: a hot topic for biotechnological applications
    journal, January 2019

    • Aulitto, Martina; Fusco, Salvatore; Limauro, Danila
    • World Journal of Microbiology and Biotechnology, Vol. 35, Issue 2
    • DOI: 10.1007/s11274-019-2591-3

    Biochemical characterization of extra- and intracellular endoxylanse from thermophilic bacterium Caldicellulosiruptor kronotskyensis
    journal, February 2016

    • Jia, Xiaojing; Qiao, Weibo; Tian, Wenli
    • Scientific Reports, Vol. 6, Issue 1
    • DOI: 10.1038/srep21672
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