High-resolution x-ray diffraction microscopy of specifically labeled yeast cells
Abstract
X-ray diffraction microscopy complements other x-ray microscopy methods by being free of lens-imposed radiation dose and resolution limits, and it allows for high-resolution imaging of biological specimens too thick to be viewed by electron microscopy. We report here the highest resolution (11-13 nm) x-ray diffraction micrograph of biological specimens, and a demonstration of molecular-specific gold labeling at different depths within cells via through-focus propagation of the reconstructed wavefield. The lectin concanavalin A conjugated to colloidal gold particles was used to label the α-mannan sugar in the cell wall of the yeast Saccharomyces cerevisiae. Cells were plunge-frozen in liquid ethane and freeze-dried, after which they were imaged whole using x-ray diffraction microscopy at 750 eV photon energy.
- Authors:
-
- Stony Brook Univ., Stony Brook, NY (United States)
- Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States). Advanced Light Source
- Northwestern Univ., Evanston, IL (United States)
- Stony Brook Univ., Stony Brook, NY (United States); Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States). Advanced Light Source; Argonne National Laboratory (ANL), Argonne, IL (United States). Advanced Photon Source
- Publication Date:
- Research Org.:
- Stony Brook Univ., NY (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES)
- OSTI Identifier:
- 1164150
- Grant/Contract Number:
- FG02-04ER46128
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Proceedings of the National Academy of Sciences of the United States of America
- Additional Journal Information:
- Journal Volume: 107; Journal Issue: 16; Journal ID: ISSN 0027-8424
- Publisher:
- National Academy of Sciences
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 36 MATERIALS SCIENCE; 77 NANOSCIENCE AND NANOTECHNOLOGY
Citation Formats
Nelson, Johanna, Huang, Xiaojing, Steinbrener, Jan, Shapiro, David, Kirz, Janos, Marchesini, Stephano, Neiman, Aaron M., Turner, Joshua J., and Jacobsen, Chris. High-resolution x-ray diffraction microscopy of specifically labeled yeast cells. United States: N. p., 2010.
Web. doi:10.1073/pnas.0910874107.
Nelson, Johanna, Huang, Xiaojing, Steinbrener, Jan, Shapiro, David, Kirz, Janos, Marchesini, Stephano, Neiman, Aaron M., Turner, Joshua J., & Jacobsen, Chris. High-resolution x-ray diffraction microscopy of specifically labeled yeast cells. United States. https://doi.org/10.1073/pnas.0910874107
Nelson, Johanna, Huang, Xiaojing, Steinbrener, Jan, Shapiro, David, Kirz, Janos, Marchesini, Stephano, Neiman, Aaron M., Turner, Joshua J., and Jacobsen, Chris. Tue .
"High-resolution x-ray diffraction microscopy of specifically labeled yeast cells". United States. https://doi.org/10.1073/pnas.0910874107. https://www.osti.gov/servlets/purl/1164150.
@article{osti_1164150,
title = {High-resolution x-ray diffraction microscopy of specifically labeled yeast cells},
author = {Nelson, Johanna and Huang, Xiaojing and Steinbrener, Jan and Shapiro, David and Kirz, Janos and Marchesini, Stephano and Neiman, Aaron M. and Turner, Joshua J. and Jacobsen, Chris},
abstractNote = {X-ray diffraction microscopy complements other x-ray microscopy methods by being free of lens-imposed radiation dose and resolution limits, and it allows for high-resolution imaging of biological specimens too thick to be viewed by electron microscopy. We report here the highest resolution (11-13 nm) x-ray diffraction micrograph of biological specimens, and a demonstration of molecular-specific gold labeling at different depths within cells via through-focus propagation of the reconstructed wavefield. The lectin concanavalin A conjugated to colloidal gold particles was used to label the α-mannan sugar in the cell wall of the yeast Saccharomyces cerevisiae. Cells were plunge-frozen in liquid ethane and freeze-dried, after which they were imaged whole using x-ray diffraction microscopy at 750 eV photon energy.},
doi = {10.1073/pnas.0910874107},
journal = {Proceedings of the National Academy of Sciences of the United States of America},
number = 16,
volume = 107,
place = {United States},
year = {Tue Apr 20 00:00:00 EDT 2010},
month = {Tue Apr 20 00:00:00 EDT 2010}
}
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