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Title: Osmotic pressures and second virial coefficients for aqueous saline solutions of lysozyme

Abstract

Experimental data at 25 °C are reported for osmotic pressures of aqueous solutions containing lysozyme and any one of the following salts: ammonium sulfate, ammonium oxalate and ammonium phosphate at ionic strength 1 or 3M. Data were obtained using a Wescor Colloid Membrane Osmometer at lysozyme concentrations from about 4 to 20 grams per liter at pH 4, 7 or 8. Osmotic second virial coefficients for lysozyme were calculated from the osmotic-pressure data. All coefficients were negative, increasing in magnitude with ionic strength. Furthermore, tesults are insensitive to the nature of the anion, but rise slightly in magnitude as the size of the anion increases.

Authors:
 [1];  [1];  [1];  [2]
  1. Univ. of California, Berkeley, CA (United States)
  2. Univ. of California, Berkeley, CA (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Publication Date:
Research Org.:
Lawrence Berkeley National Laboratory, Berkeley, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)
OSTI Identifier:
751781
Report Number(s):
LBNL-44257
Journal ID: ISSN 0378-3812; R&D Project: 402201
Grant/Contract Number:  
AC03-76SF00098
Resource Type:
Accepted Manuscript
Journal Name:
Fluid Phase Equilibria
Additional Journal Information:
Journal Volume: 168; Journal Issue: 2; Journal ID: ISSN 0378-3812
Publisher:
Elsevier
Country of Publication:
United States
Language:
English
Subject:
37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; proteins; salts; intermolecular interactions; potentials of mean force; osmotic-pressure data; experimental method

Citation Formats

Moon, Y. U., Anderson, C. O., Blanch, H. W., and Prausnitz, J. M. Osmotic pressures and second virial coefficients for aqueous saline solutions of lysozyme. United States: N. p., 2000. Web. doi:10.1016/S0378-3812(99)00337-4.
Moon, Y. U., Anderson, C. O., Blanch, H. W., & Prausnitz, J. M. Osmotic pressures and second virial coefficients for aqueous saline solutions of lysozyme. United States. doi:10.1016/S0378-3812(99)00337-4.
Moon, Y. U., Anderson, C. O., Blanch, H. W., and Prausnitz, J. M. Mon . "Osmotic pressures and second virial coefficients for aqueous saline solutions of lysozyme". United States. doi:10.1016/S0378-3812(99)00337-4. https://www.osti.gov/servlets/purl/751781.
@article{osti_751781,
title = {Osmotic pressures and second virial coefficients for aqueous saline solutions of lysozyme},
author = {Moon, Y. U. and Anderson, C. O. and Blanch, H. W. and Prausnitz, J. M.},
abstractNote = {Experimental data at 25 °C are reported for osmotic pressures of aqueous solutions containing lysozyme and any one of the following salts: ammonium sulfate, ammonium oxalate and ammonium phosphate at ionic strength 1 or 3M. Data were obtained using a Wescor Colloid Membrane Osmometer at lysozyme concentrations from about 4 to 20 grams per liter at pH 4, 7 or 8. Osmotic second virial coefficients for lysozyme were calculated from the osmotic-pressure data. All coefficients were negative, increasing in magnitude with ionic strength. Furthermore, tesults are insensitive to the nature of the anion, but rise slightly in magnitude as the size of the anion increases.},
doi = {10.1016/S0378-3812(99)00337-4},
journal = {Fluid Phase Equilibria},
number = 2,
volume = 168,
place = {United States},
year = {2000},
month = {3}
}

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