Investigating the quaternary structure of a homomultimeric catechol 1,2-dioxygenase: An integrative structural biology study

Almeida-Juarez, Arisbeth Guadalupe; Chodankar, Shirish; Pardo-López, Liliana; Zavala-Padilla, Guadalupe; Rudiño-Piñera, Enrique; De March, ed., Matteo

doi:10.1371/journal.pone.0315992

Title: Investigating the quaternary structure of a homomultimeric catechol 1,2-dioxygenase: An integrative structural biology study

Journal Article · 05 May 2025 · PLoS ONE

DOI: https://doi.org/10.1371/journal.pone.0315992 · OSTI ID:2564469

Almeida-Juarez, Arisbeth Guadalupe; Chodankar, Shirish; Pardo-López, Liliana; Zavala-Padilla, Guadalupe; Rudiño-Piñera, Enrique; De March, ed., Matteo

The structural analysis of catechol 1,2 dioxygenase from Stutzerimonas frequens GOM2, SfC12DO, was conducted using various structural techniques. SEC-SAXS experiments revealed that SfC12DO, after lyophilization and reconstitution processes, can form multiple enzymatically active oligomers, including dimers, tetramers, and octamers. These findings differ from previous studies, which reported active dimers in homologous counterparts with available crystallographic structures, or trimers observed exclusively in solution for SfC12DO and its homologous isoA C12DO from Acinetobacter radioresistens under low ionic strength conditions. In some cases, tetramers were also reported, such as for the Rodococcus erythropolis C12DO. The combined results of Small-Angle X-ray Scattering, Dynamic Light Scattering, and Transmission Electron Microscopy experiments provided additional insights into these active oligomers’ shape and molecular organization in an aqueous solution. These results highlight the oligomeric structural plasticity of SfC12DO, proving that it can exist in different oligomeric forms depending on the physicochemical characteristics of the solutions in which the experiments were performed. Remarkably, regardless of its oligomeric state, SfC12DO maintains its enzymatic activity even after prior lyophilization. All these characteristics make SfC12DO a putative candidate for bioremediation applications in polluted soils or waters.

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Sponsoring Organization:: USDOE

Grant/Contract Number:: SC0012704

OSTI ID:: 2564469

Journal Information:: PLoS ONE, Journal Name: PLoS ONE Journal Issue: 5 Vol. 20; ISSN 1932-6203

Publisher:: Public Library of Science (PLoS)Copyright Statement

Country of Publication:: United States

Language:: English

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