Oral microbiome and mycobiome dynamics in cancer therapy-induced oral mucositis

Nodit, Laurentia; Kelley, Joseph R.; Panella, Timothy J.; Bruckbauer, Antje; Nodit, Paul G.; Shope, Grace A.; Peyton, Kellie; Klingeman, Dawn M.; Zaretzki, Russell; Carrell, Alyssa; Podar, Mircea

doi:10.1038/s41597-025-04671-z

Title: Oral microbiome and mycobiome dynamics in cancer therapy-induced oral mucositis

Journal Article · 19 March 2025 · Scientific Data (Online)

DOI: https://doi.org/10.1038/s41597-025-04671-z · OSTI ID:2533582

Nodit, Laurentia ^[1]; Kelley, Joseph R. ^[2]; Panella, Timothy J. ^[1]; Bruckbauer, Antje ^[1]; Nodit, Paul G. ^[3]; Shope, Grace A. ^[1]; Peyton, Kellie ^[4];

^[4]; Zaretzki, Russell ^[1]; Carrell, Alyssa ^[4];

^[4]

University of Tennessee, Knoxville, TN (United States)
University of Tennessee, Knoxville, TN (United States); Genesis Care of North Carolina, Asheville, NC (United States)
University of Tennessee, Memphis, TN (United States)
Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Cancer therapy-induced oral mucositis is a frequent major oncological problem, secondary to cytotoxicity of chemo-radiation treatment. Oral mucositis commonly occurs 7–10 days after initiation of therapy; it is a dose-limiting side effect causing significant pain, eating difficulty, need for parenteral nutrition and a rise of infections. The pathobiology derives from complex interactions between the epithelial component, inflammation, and the oral microbiome. Our longitudinal study analysed the dynamics of the oral microbiome (bacteria and fungi) in nineteen patients undergoing chemo-radiation therapy for oral and oropharyngeal squamous cell carcinoma as compared to healthy volunteers. The microbiome was characterized in multiple oral sample types using rRNA and ITS sequence amplicons and followed the treatment regimens. Microbial taxonomic diversity and relative abundance may be correlated with disease state, type of treatment and responses. Identification of microbial-host interactions could lead to further therapeutic interventions of mucositis to re-establish normal flora and promote patients’ health. Data presented here could enhance, complement and diversify other studies that link microbiomes to oral disease, prophylactics, treatments, and outcome.

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Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE; USDOE Laboratory Directed Research and Development (LDRD) Program

Grant/Contract Number:: AC05-00OR22725

OSTI ID:: 2533582

Journal Information:: Scientific Data (Online), Journal Name: Scientific Data (Online) Journal Issue: 1 Vol. 12; ISSN 2052-4463

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

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