A fungal protein organizes both glycogen and cell wall glucans

Loza, Liza; Doering, Tamara L.

doi:10.1073/pnas.2319707121

Title: A fungal protein organizes both glycogen and cell wall glucans

Journal Article · 13 May 2024 · Proceedings of the National Academy of Sciences of the United States of America

DOI: https://doi.org/10.1073/pnas.2319707121 · OSTI ID:2530410

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Washington University in St. Louis School of Medicine, MO (United States)

Glycogen is a glucose storage molecule composed of branched α-1,4-glucan chains, best known as an energy reserve that can be broken down to fuel central metabolism. Because fungal cells have a specialized need for glucose in building cell wall glucans, we investigated whether glycogen is used for this process. For these studies, we focused on the pathogenic yeast Cryptococcus neoformans, which causes ~150,000 deaths per year worldwide. We identified two proteins that influence formation of both glycogen and the cell wall: glycogenin (Glg1), which initiates glycogen synthesis, and a protein that we call Glucan organizing enzyme 1 (Goe1). We found that cells missing Glg1 lack α-1,4-glucan in their walls, indicating that this material is derived from glycogen. Without Goe1, glycogen rosettes are mislocalized and β-1,3-glucan in the cell wall is reduced. Altogether, our results provide mechanisms for a close association between glycogen and cell wall.

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Research Organization:: University of Georgia, Athens, GA (United States)

Sponsoring Organization:: USDOE

Grant/Contract Number:: SC0015662

OSTI ID:: 2530410

Journal Information:: Proceedings of the National Academy of Sciences of the United States of America, Journal Name: Proceedings of the National Academy of Sciences of the United States of America Journal Issue: 21 Vol. 121; ISSN 0027-8424

Publisher:: National Academy of SciencesCopyright Statement

Country of Publication:: United States

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
Cryptococcus neoformans
cell wall
glycogen
glycogenin
glycosytransferase

Title: A fungal protein organizes both glycogen and cell wall glucans

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