Identification and functional analysis of strigolactone pathway genes regulating tillering traits in sugarcane

Qi, Yiying; Feng, Xiaoxi; Ding, Hongyan; Lin, Dadong; Lan, Yuhong; Zhang, Yixing; Akbar, Sehrish; Shi, Huihong; Li, Zhen; Gao, Ruiting; Hua, Xiuting; Wang, Yuhao; Zhang, Jisen

doi:10.1093/pcp/pcae146

Title: Identification and functional analysis of strigolactone pathway genes regulating tillering traits in sugarcane

Journal Article · 18 December 2024 · Plant and Cell Physiology

DOI: https://doi.org/10.1093/pcp/pcae146 · OSTI ID:2526634

Qi, Yiying; Feng, Xiaoxi; Ding, Hongyan; Lin, Dadong;

; Zhang, Yixing;

;

; Li, Zhen; Gao, Ruiting;

; Wang, Yuhao;

Abstract Saccharum officinarum and Saccharum spontaneum are two fundamental species of modern sugarcane cultivars, exhibiting divergent tillering patterns crucial for sugarcane architecture and yield. Strigolactones (SLs), a class of plant hormones, are considered to play a central role in shaping plant form and regulating tillering. Our study highlights the distinct tillering patterns observed between S. officinarum and S. spontaneum and implicates significant differences in SL levels in root exudates between the two species. Treatment with rac-GR24 (an artificial SL analog) suppressed tillering in S. spontaneum. Based on transcriptome analysis, we focused on two genes, TRANSCRIPTION ELONGATION FACTOR 1 (TEF1) and CIRCADIAN CLOCK ASSOCIATED1 (CCA1), which show higher expression in S. spontaneum or S. officinarum, respectively. While the overexpression of SoCCA1 did not lead to significant phenotypic differences, overexpression of SsTEF1 in rice stimulated tillering and inhibited plant height, demonstrating its role in tillering regulation. However, the overexpression of suggests that SoCCA1 may not be the key regulator of sugarcane tillering. Yeast one-hybrid assays identified four transcription factors (TFs) regulating SsTEF1 and four and five TFs regulating SsCCA1 and SoCCA1. This study provides a theoretical foundation for deciphering the molecular mechanisms underlying the different tillering behaviors between S. officinarum and S. spontaneum, providing valuable insights for the molecular-based design of sugarcane breeding strategies.

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Sponsoring Organization:: USDOE Office of Electricity (OE), Advanced Grid Research & Development. Power Systems Engineering Research

OSTI ID:: 2526634

Journal Information:: Plant and Cell Physiology, Journal Name: Plant and Cell Physiology Journal Issue: 2 Vol. 66; ISSN 0032-0781

Publisher:: Oxford University PressCopyright Statement

Country of Publication:: Japan

Language:: English

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