Salinity-Induced Photorespiration in Populus Vascular Tissues Facilitate Nitrogen Reallocation

Wilhelmi, Maria del Mar Rubio; Maneejantra, Nuchada; Balasubramanian, Vimal Kumar; Purvine, Samuel O.; Williams, Sarai; DiFazio, Stephen; Stewart Jr., C. Neal; Ahkami, Amir H.; Blumwald, Eduardo

doi:10.1111/pce.15180

Title: Salinity-Induced Photorespiration in Populus Vascular Tissues Facilitate Nitrogen Reallocation

Journal Article · 30 September 2024 · Plant, Cell and Environment

DOI: https://doi.org/10.1111/pce.15180 · OSTI ID:2525930

Wilhelmi, Maria del Mar Rubio ^[1]; Maneejantra, Nuchada ^[1]; Balasubramanian, Vimal Kumar ^[2]; Purvine, Samuel O. ^[2]; Williams, Sarai ^[2]; DiFazio, Stephen ^[3]; Stewart Jr., C. Neal ^[4]; Ahkami, Amir H. ^[5];

^[1]

Univ. of California, Davis, CA (United States)
Pacific Northwest National Laboratory (PNNL), Richland, WA (United States). Environmental Molecular Sciences Laboratory (EMSL)
West Virginia Univ., Morgantown, WV (United States)
Univ. of Tennessee, Knoxville, TN (United States)
Pacific Northwest National Laboratory (PNNL), Richland, WA (United States). Environmental Molecular Sciences Laboratory (EMSL); Washington State Univ., Pullman, WA (United States)

Adaptation to abiotic stress is critical for the survival of perennial tree species. Salinity affects plant growth and productivity by interfering with major biosynthetic processes. Detrimental effects of salinity may vary between different plant tissues and cell types. However, spatial molecular mechanisms controlling plant responses to salinity stress are not yet thoroughly understood in perennial trees. Here, we used laser capture microdissection in clones of Populus tremula x alba to isolate palisade and vascular cells of intermediary leaf from plants exposed to 150 mM NaCl for 10 days, followed by a recovery period. Cell-specific changes in proteins and metabolites were determined. Salinity induced a vascular-specific accumulation of proteins associated with photorespiration, and the accumulation of serine, 3-phosphoglycerate and NH₄⁺ suggesting changes in N metabolism. Accumulation of the GLUTAMINE SYNTHETASE 2 protein, and increased GS1.1 gene expression, indicated that NH₄⁺ produced in photorespiration was assimilated to glutamine, the main amino acid translocated in Populus trees. Further analysis of total soluble proteins in stems and roots showed the accumulation of bark storage proteins induced by the salinity treatments. Collectively, our results suggest that the salt-induced photorespiration in vascular cells mediates N-reallocation in Populus, an essential process for the adaptation of trees to adverse conditions.

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Research Organization:: Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES). Scientific User Facilities (SUF); USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: AC05-76RL01830; SC0018347

OSTI ID:: 2525930

Report Number(s):: PNNL-SA--197282

Journal Information:: Plant, Cell and Environment, Journal Name: Plant, Cell and Environment Journal Issue: 1 Vol. 48; ISSN 0140-7791

Publisher:: WileyCopyright Statement

Country of Publication:: United States

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
abiotic stress
ammonium
cell type‐specific proteomics
nitrogen
palisade
photorespiration
resource reallocation
salinity
trees
vascular

Title: Salinity-Induced Photorespiration in Populus Vascular Tissues Facilitate Nitrogen Reallocation

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