Protein ligation for the assembly and study of nonribosomal peptide synthetase megaenzymes

Pistofidis, Angelos; Schmeing, T. Martin

doi:10.1039/D4CB00306C

Title: Protein ligation for the assembly and study of nonribosomal peptide synthetase megaenzymes

Journal Article · 02 April 2025 · RSC Chemical Biology

DOI: https://doi.org/10.1039/D4CB00306C · OSTI ID:2516758

Pistofidis, Angelos ^[1];

^[1]

Department of Biochemistry and Centre de Recherche en Biologie Structurale, McGill University, Montréal, QC, H3G 0B1, Canada

NRPS modules are expressed and complexed with substrate analogues separately then ligated to stall at a specific catalytic step, investigated using crystallography.

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Sponsoring Organization:: USDOE

Grant/Contract Number:: AC02-06CH11357

OSTI ID:: 2516758

Journal Information:: RSC Chemical Biology, Journal Name: RSC Chemical Biology Journal Issue: 4 Vol. 6; ISSN 2633-0679; ISSN RCBSAO

Publisher:: Royal Society of Chemistry (RSC)Copyright Statement

Country of Publication:: United Kingdom

Language:: English

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