Microbial partner (MiPner) analysis

Bennetzen, Jeffrey L.; Fernandez-Canela, Josue; Elmgreen, Vienna; McCann, Shaugnessy R.; Norris, Mary E.; Deng, Xiangyu; Brailey-Crane, Philip

doi:10.3389/frmbi.2024.1500798

Title: Microbial partner (MiPner) analysis

Journal Article · 07 February 2025 · Frontiers in Microbiomes

DOI: https://doi.org/10.3389/frmbi.2024.1500798 · OSTI ID:2513510

Bennetzen, Jeffrey L.; Fernandez-Canela, Josue; Elmgreen, Vienna; McCann, Shaugnessy R.; Norris, Mary E.; Deng, Xiangyu; Brailey-Crane, Philip

Introduction Although a few bacteria have been studied in great depth, relatively little is known about the characteristics of microbe-microbe interactions that occur within ecosystems on a daily basis. A simple, robust technique was developed to set up the foundation for investigating pairwise bacterial-bacterial interactions, using cell-cell binding as a self-selective mechanism to identify interesting bacterial species pairs. Methods Using a Serratia marcescens strain (SMC43) isolated from Georgia soil as a “bait”, specific bacteria were purified by their specificity in binding SMC43 bacteria that were themselves attached to a wooden applicator stick. Results The isolated Microbial Partners (MiPners) were greatly enriched for members of the genera Sphingobium and Caulobacter . Two streaked MiPners were unable to grow on the plates employed after separation from SMC43to be separated from, and grow on the plate type tested without, SMC43. Discussion This suggests that the MiPner technology will be one strategy for purifying bacteria that were previously recalcitrant to culturing.

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Sponsoring Organization:: USDOE

Grant/Contract Number:: SC0021386

OSTI ID:: 2513510

Journal Information:: Frontiers in Microbiomes, Journal Name: Frontiers in Microbiomes Vol. 3; ISSN 2813-4338

Publisher:: Frontiers Media SACopyright Statement

Country of Publication:: Switzerland

Language:: English

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