A genome-scale metabolic model for the denitrifying bacterium Thauera sp. MZ1T accurately predicts degradation of pollutants and production of polymers

Tec-Campos, Diego; Tibocha-Bonilla, Juan D.; Jiang, Celina; Passi, Anurag; Thiruppathy, Deepan; Zuñiga, Cristal; Posadas, Camila; Zepeda, Alejandro; Zengler, Karsten; Laxman, ed., Sunil

doi:10.1371/journal.pcbi.1012736

Title: A genome-scale metabolic model for the denitrifying bacterium Thauera sp. MZ1T accurately predicts degradation of pollutants and production of polymers

Journal Article · 06 January 2025 · PLoS Computational Biology (Online)

DOI: https://doi.org/10.1371/journal.pcbi.1012736 · OSTI ID:2503550

; Tibocha-Bonilla, Juan D.; Jiang, Celina;

;

; Zuñiga, Cristal; Posadas, Camila;

;

; Laxman, ed., Sunil

The denitrifying bacterium Thauera sp . MZ1T, a common member of microbial communities in wastewater treatment facilities, can produce different compounds from a range of carbon (C) and nitrogen (N) sources under aerobic and anaerobic conditions. In these different conditions, Thauera modifies its metabolism to produce different compounds that influence the microbial community. In particular, Thauera sp . MZ1T produces different exopolysaccharides with floc-forming properties, impacting the physical disposition of wastewater consortia and the efficiency of nutrient assimilation by the microbial community. Under N-limiting conditions, Thauera sp . MZ1T decreases its growth rate and accelerates the accumulation of polyhydroxyalkanoate-related (PHA) compounds including polyhydroxybutyrate (PHB), which plays a fundamental role as C and energy storage in this β-proteobacterium. However, the metabolic mechanisms employed by Thauera sp . MZ1T to assimilate and catabolize many of the different C and N sources under aerobic and anaerobic conditions remain unknown. Systems biology approaches such as genome-scale metabolic modeling have been successfully used to unveil complex metabolic mechanisms for various microorganisms. Here, we developed a comprehensive metabolic model (M-model) for Thauera sp . MZ1T ( i Thauera861), consisting of 1,744 metabolites, 2,384 reactions, and 861 genes. We validated the model experimentally using over 70 different C and N sources under both aerobic and anaerobic conditions. i Thauera861 achieved a prediction accuracy of 95% for growth on various C and N sources and close to 85% for assimilation of aromatic compounds under denitrifying conditions. The M-model was subsequently deployed to determine the effects of substrates, oxygen presence, and the C:N ratio on the production of PHB and exopolysaccharides (EPS), showing the highest polymer yields are achieved with nucleotides and amino acids under aerobic conditions. This comprehensive M-model will help reveal the metabolic processes by which this ubiquitous species influences communities in wastewater treatment systems and natural environments.

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Sponsoring Organization:: USDOE

Grant/Contract Number:: AC36-08GO28308; SC0021234

OSTI ID:: 2503550

Journal Information:: PLoS Computational Biology (Online), Journal Name: PLoS Computational Biology (Online) Journal Issue: 1 Vol. 21; ISSN 1553-7358

Publisher:: Public Library of Science (PLoS)Copyright Statement

Country of Publication:: United States

Language:: English

References (69)

Inhibition and gene expression of Nitrosomonas europaea biofilms exposed to phenol and toluene Lauchnor, Ellen G.; Radniecki, Tyler S.; Semprini, Lewis Biotechnology and Bioengineering, Vol. 108, Issue 4 https://doi.org/10.1002/bit.22999	journal	November 2010
Structure of Catechol 2,3-Dioxygenase Gene Encoded in Chromosomal DNA ofPseudomonas putidaKF715 Lee, Jeongrai; Oh, Jungmi; Min, Kyung Rak Biochemical and Biophysical Research Communications, Vol. 224, Issue 3 https://doi.org/10.1006/bbrc.1996.1108	journal	July 1996
Structure of Catechol 2,3-Dioxygenase Gene fromAlcaligenes eutrophus335 Kang, Bo-Seong; Ha, Joo Young; Lim, Jong-Chul Biochemical and Biophysical Research Communications, Vol. 245, Issue 3 https://doi.org/10.1006/bbrc.1998.8511	journal	April 1998
The Family Rhodocyclaceae Oren, Aharon The Prokaryotes https://doi.org/10.1007/978-3-642-30197-1_292	book	January 2014
Phylogenetic and metabolic diversity of bacteria degrading aromatic compounds under denitrifying conditions, and description of Thauera phenylacetica sp. nov., Thauera aminoaromatica sp. nov., and Azoarcus buckelii sp. nov. Mechichi, Tahar; Stackebrandt, Erko; Gad'on, Nasser Archives of Microbiology, Vol. 178, Issue 1 https://doi.org/10.1007/s00203-002-0422-6	journal	July 2002
Two distinct pathways for anaerobic degradation of aromatic compounds in the denitrifying bacterium Thauera aromatica strain AR-1 Philipp, Bodo; Schink, Bernhard Archives of Microbiology, Vol. 173, Issue 2 https://doi.org/10.1007/s002039900112	journal	December 1999
Reconstructing a Thauera genome from a hydrogenotrophic-denitrifying consortium using metagenomic sequence data Mao, Yanping; Xia, Yu; Wang, Zhiping Applied Microbiology and Biotechnology, Vol. 98, Issue 15 https://doi.org/10.1007/s00253-014-5756-x	journal	April 2014
Versatile aromatic compound-degrading capacity and microdiversity of Thauera strains isolated from a coking wastewater treatment bioreactor Mao, Yuejian; Zhang, Xiaojun; Xia, Xi Journal of Industrial Microbiology & Biotechnology, Vol. 37, Issue 9 https://doi.org/10.1007/s10295-010-0740-7	journal	May 2010
Production of poly-β-hydroxybutyrate by activated sludge in sequencing batch reactor under aerobic conditions Li, Xiaoyan; Jin, Zijing; Qian, Yongyu Journal of Wuhan University of Technology-Mater. Sci. Ed., Vol. 32, Issue 3 https://doi.org/10.1007/s11595-017-1660-4	journal	June 2017
Effect of Cycle Time on Polyhydroxybutyrate (PHB) Production in Aerobic Mixed Cultures Ozdemir, Sebnem; Akman, Dilek; Cirik, Kevser Applied Biochemistry and Biotechnology, Vol. 172, Issue 5 https://doi.org/10.1007/s12010-013-0676-2	journal	January 2014
Creating a synthetic lichen: Mutualistic co-culture of fungi and extracellular polysaccharide-secreting cyanobacterium Nostoc PCC 7413 Li, Tingting; Jiang, Liqun; Hu, Yifeng Algal Research, Vol. 45 https://doi.org/10.1016/j.algal.2019.101755	journal	January 2020
Formation of aerobic granules and their PHB production at various substrate and ammonium concentrations Fang, Fang; Liu, Xian-Wei; Xu, Juan Bioresource Technology, Vol. 100, Issue 1 https://doi.org/10.1016/j.biortech.2008.06.016	journal	January 2009
Rhodopseudomonas palustris CGA009 polyhydroxybutyrate production from a lignin aromatic and quantification via flow cytometry Brown, Brandi; Immethun, Cheryl; Wilkins, Mark Bioresource Technology Reports, Vol. 11 https://doi.org/10.1016/j.biteb.2020.100474	journal	September 2020
Complete nitrogen removal and electricity production in Thauera-dominated air-cathode single chambered microbial fuel cell Yang, Nuan; Zhan, Guoqiang; Li, Daping Chemical Engineering Journal, Vol. 356 https://doi.org/10.1016/j.cej.2018.08.161	journal	January 2019
Tracking multiple aromatic compounds in a full-scale coking wastewater reclamation plant: Interaction with biological and advanced treatments Ren, Jing; Li, Jianfeng; Li, Jianguo Chemosphere, Vol. 222 https://doi.org/10.1016/j.chemosphere.2019.01.179	journal	May 2019
Molecular mechanisms through which different carbon sources affect denitrification by Thauera linaloolentis: Electron generation, transfer, and competition Wei, Qi; Zhang, Jinsen; Luo, Fangzhou Environment International, Vol. 170 https://doi.org/10.1016/j.envint.2022.107598	journal	December 2022
Dissimilatory nitrate/nitrite reduction to ammonium (DNRA) pathway dominates nitrate reduction processes in rhizosphere and non-rhizosphere of four fertilized farmland soil Pan, Huawei; Qin, Yu; Wang, Yuantao Environmental Research, Vol. 186 https://doi.org/10.1016/j.envres.2020.109612	journal	July 2020
Exopolysaccharide production by the marine bacterium Alteromonas macleodii Mo169 using fruit pulp waste as the sole carbon source Concórdio-Reis, Patrícia; Serafim, Bruno; Pereira, João R. Environmental Technology & Innovation, Vol. 30 https://doi.org/10.1016/j.eti.2023.103090	journal	May 2023
Optimization of polyhydroxybutyrate (PHB) production by excess activated sludge and microbial community analysis Liu, Zhenggui; Wang, Yuanpeng; He, Ning Journal of Hazardous Materials, Vol. 185, Issue 1 https://doi.org/10.1016/j.jhazmat.2010.08.003	journal	January 2011
Production of poly-β-hydroxybutyrate (PHB) by Methylobacterium organophilum isolated from a methanotrophic consortium in a two-phase partition bioreactor Zúñiga, C.; Morales, M.; Le Borgne, S. Journal of Hazardous Materials, Vol. 190, Issue 1-3 https://doi.org/10.1016/j.jhazmat.2011.04.011	journal	June 2011
Effect of C/N ratio on the PHA accumulation capability of microbial mixed culture fed with leachates from the organic fraction of municipal solid waste (OFMSW) Sánchez Valencia, Angel Iván; Rojas Zamora, Ulises; Meraz Rodríguez, Mónica Journal of Water Process Engineering, Vol. 40 https://doi.org/10.1016/j.jwpe.2021.101975	journal	April 2021
Modeling of nitrogen fixation and polymer production in the heterotrophic diazotroph Azotobacter vinelandii DJ Tec-Campos, Diego; Zuñiga, Cristal; Passi, Anurag Metabolic Engineering Communications, Vol. 11 https://doi.org/10.1016/j.mec.2020.e00132	journal	December 2020
Thauera sp. Sel9, a new bacterial strain for polyhydroxyalkanoates production from volatile fatty acids Andreolli, Marco; Scerbacov, Vadim; Frison, Nicola New Biotechnology, Vol. 72 https://doi.org/10.1016/j.nbt.2022.09.004	journal	December 2022
The impact of carbon to nitrogen ratios and pH on the microbial prevalence and polyhydroxybutyrate production levels using a mixed microbial starter culture Zhou, Wen; Colpa, Dana Irene; Geurkink, Bert Science of The Total Environment, Vol. 811 https://doi.org/10.1016/j.scitotenv.2021.152341 152341	journal	March 2022
Influence of the C/N ratio on the performance of polyhydroxybutyrate (PHB) producing sequencing batch reactors at short SRTs Johnson, Katja; Kleerebezem, Robbert; van Loosdrecht, Mark C. M. Water Research, Vol. 44, Issue 7 https://doi.org/10.1016/j.watres.2009.12.031	journal	April 2010
Aromatic compounds lead to increased abundance of antibiotic resistance genes in wastewater treatment bioreactors Xia, Juntao; Sun, Haohao; Zhang, Xu-xiang Water Research, Vol. 166 https://doi.org/10.1016/j.watres.2019.115073	journal	December 2019
Genetic potential for exopolysaccharide synthesis in activated sludge bacteria uncovered by genome-resolved metagenomics Dueholm, Morten Kam Dahl; Besteman, Maaike; Zeuner, Emil Juel Water Research, Vol. 229 https://doi.org/10.1016/j.watres.2022.119485	journal	February 2023
Global Patterns and Drivers of Soil Dissimilatory Nitrate Reduction to Ammonium Cheng, Yi; Elrys, Ahmed S.; Merwad, Abdel-Rahman M. Environmental Science & Technology, Vol. 56, Issue 6 https://doi.org/10.1021/acs.est.1c07997	journal	February 2022
Physiological and Transcriptional Responses of Nitrosomonas europaea to Toluene and Benzene Inhibition Radniecki, Tyler S.; Dolan, Mark E.; Semprini, Lewis Environmental Science & Technology, Vol. 42, Issue 11 https://doi.org/10.1021/es702623s	journal	June 2008
What is flux balance analysis? Orth, Jeffrey D.; Thiele, Ines; Palsson, Bernhard Ø Nature Biotechnology, Vol. 28, Issue 3 https://doi.org/10.1038/nbt.1614	journal	March 2010
iML1515, a knowledgebase that computes Escherichia coli traits Monk, Jonathan M.; Lloyd, Colton J.; Brunk, Elizabeth Nature Biotechnology, Vol. 35, Issue 10 https://doi.org/10.1038/nbt.3956	journal	October 2017
KBase: The United States Department of Energy Systems Biology Knowledgebase Arkin, Adam P.; Cottingham, Robert W.; Henry, Christopher S. Nature Biotechnology, Vol. 36, Issue 7 https://doi.org/10.1038/nbt.4163	journal	July 2018
Linking metabolic phenotypes to pathogenic traits among “Candidatus Liberibacter asiaticus” and its hosts Zuñiga, Cristal; Peacock, Beth; Liang, Bo npj Systems Biology and Applications, Vol. 6, Issue 1 https://doi.org/10.1038/s41540-020-00142-w	journal	August 2020
Creation and analysis of biochemical constraint-based models using the COBRA Toolbox v.3.0 Heirendt, Laurent; Arreckx, Sylvain; Pfau, Thomas Nature Protocols, Vol. 14, Issue 3 https://doi.org/10.1038/s41596-018-0098-2	journal	February 2019
Biomineralization of selenium by the selenate-respiring bacterium Thauera selenatis Butler, Clive S.; Debieux, Charles M.; Dridge, Elizabeth J. Biochemical Society Transactions, Vol. 40, Issue 6 https://doi.org/10.1042/BST20120087	journal	November 2012
The BioCyc collection of microbial genomes and metabolic pathways Karp, Peter D.; Billington, Richard; Caspi, Ron Briefings in Bioinformatics, Vol. 20, Issue 4 https://doi.org/10.1093/bib/bbx085	journal	August 2017
KEGG: Kyoto Encyclopedia of Genes and Genomes Kanehisa, Minoru; Goto, Susumu Nucleic Acids Research, Vol. 28, Issue 1, p. 27-30 https://doi.org/10.1093/nar/28.1.27	journal	January 2000
BRENDA, the ELIXIR core data resource in 2021: new developments and updates Chang, Antje; Jeske, Lisa; Ulbrich, Sandra Nucleic Acids Research, Vol. 49, Issue D1 https://doi.org/10.1093/nar/gkaa1025	journal	November 2020
UniProt: the universal protein knowledgebase in 2021 Bateman, Alex; Martin, Maria-Jesus; Orchard, Sandra Nucleic Acids Research, Vol. 49, Issue D1 https://doi.org/10.1093/nar/gkaa1100	journal	November 2020
The ModelSEED Biochemistry Database for the integration of metabolic annotations and the reconstruction, comparison and analysis of metabolic models for plants, fungi and microbes Seaver, Samuel M. D.; Liu, Filipe; Zhang, Qizhi Nucleic Acids Research, Vol. 49, Issue D1 https://doi.org/10.1093/nar/gkaa746	journal	September 2020
MetaNetX/MNXref: unified namespace for metabolites and biochemical reactions in the context of metabolic models Moretti, Sébastien; Tran, Van Du T.; Mehl, Florence Nucleic Acids Research, Vol. 49, Issue D1 https://doi.org/10.1093/nar/gkaa992	journal	November 2020
PubChem 2023 update Kim, Sunghwan; Chen, Jie; Cheng, Tiejun Nucleic Acids Research, Vol. 51, Issue D1 https://doi.org/10.1093/nar/gkac956	journal	October 2022
The MetaCyc database of metabolic pathways and enzymes and the BioCyc collection of Pathway/Genome Databases Caspi, Ron; Altman, Tomer; Billington, Richard Nucleic Acids Research, Vol. 42, Issue D1 https://doi.org/10.1093/nar/gkt1103	journal	November 2013
BiGG Models: A platform for integrating, standardizing and sharing genome-scale models King, Zachary A.; Lu, Justin; Dräger, Andreas Nucleic Acids Research, Vol. 44, Issue D1 https://doi.org/10.1093/nar/gkv1049	journal	October 2015
Improvements to PATRIC, the all-bacterial Bioinformatics Database and Analysis Resource Center Wattam, Alice R.; Davis, James J.; Assaf, Rida Nucleic Acids Research, Vol. 45, Issue D1 https://doi.org/10.1093/nar/gkw1017	journal	November 2016
TransportDB 2.0: a database for exploring membrane transporters in sequenced genomes from all domains of life Elbourne, Liam D. H.; Tetu, Sasha G.; Hassan, Karl A. Nucleic Acids Research, Vol. 45, Issue D1 https://doi.org/10.1093/nar/gkw1068	journal	November 2016
Genome-Scale Metabolic Model for the Green Alga Chlorella vulgaris UTEX 395 Accurately Predicts Phenotypes under Autotrophic, Heterotrophic, and Mixotrophic Growth Conditions Zuñiga, Cristal; Li, Chien-Ting; Huelsman, Tyler Plant Physiology, Vol. 172, Issue 1 https://doi.org/10.1104/pp.16.00593	journal	July 2016
Strains in the genus Thauera exhibit remarkably different denitrification regulatory phenotypes Liu, Binbin; Mao, Yuejian; Bergaust, Linda Environmental Microbiology, Vol. 15, Issue 10 https://doi.org/10.1111/1462-2920.12142	journal	May 2013
Food waste conversion to microbial polyhydroxyalkanoates Nielsen, Chad; Rahman, Asif; Rehman, Asad Ur Microbial Biotechnology, Vol. 10, Issue 6 https://doi.org/10.1111/1751-7915.12776	journal	July 2017
Analysis and glycosyl composition of the exopolysaccharide isolated from the floc-forming wastewater bacterium Thauera sp. MZ1T Allen, Michael S.; Welch, Karen T.; Prebyl, Benjamin S. Environmental Microbiology, Vol. 6, Issue 8 https://doi.org/10.1111/j.1462-2920.2004.00615.x	journal	August 2004
Anaerobic metabolism of aromatic compounds via the benzoyl-CoA pathway Harwood, Caroline S.; Burchhardt, Gerhard; Herrmann, Heidrun FEMS Microbiology Reviews, Vol. 22, Issue 5 https://doi.org/10.1111/j.1574-6976.1998.tb00380.x	journal	December 1998
Flocculation-Related Gene Identification by Whole-Genome Sequencing of Thauera aminoaromatica MZ1T Floc-Defective Mutants Prombutara, P.; Allen, M. S. Applied and Environmental Microbiology, Vol. 82, Issue 6 https://doi.org/10.1128/AEM.02917-15	journal	December 2015
The Role of Benzoate in Anaerobic Degradation of Terephthalate Kleerebezem, Robbert; Pol, Look W. Hulshoff; Lettinga, Gatze Applied and Environmental Microbiology, Vol. 65, Issue 3 https://doi.org/10.1128/AEM.65.3.1161-1167.1999	journal	March 1999
An Experimentally Validated Genome-Scale Metabolic Reconstruction of Klebsiella pneumoniae MGH 78578, iYL1228 Liao, Y. -C.; Huang, T. -W.; Chen, F. -C. Journal of Bacteriology, Vol. 193, Issue 7 https://doi.org/10.1128/JB.01218-10	journal	February 2011
Physically associated enzymes produce and metabolize 2-hydroxy-2,4-dienoate, a chemically unstable intermediate formed in catechol metabolism via meta cleavage in Pseudomonas putida. Harayama, S.; Rekik, M.; Ngai, K. L. Journal of Bacteriology, Vol. 171, Issue 11 https://doi.org/10.1128/jb.171.11.6251-6258.1989	journal	November 1989
Anaerobic degradation of 2-aminobenzoic acid (anthranilic acid) via benzoyl-coenzyme A (CoA) and cyclohex-1-enecarboxyl-CoA in a denitrifying bacterium Lochmeyer, C.; Koch, J.; Fuchs, G. Journal of Bacteriology, Vol. 174, Issue 11 https://doi.org/10.1128/jb.174.11.3621-3628.1992	journal	June 1992
BLAST+: architecture and applications Camacho, Christiam; Coulouris, George; Avagyan, Vahram BMC Bioinformatics, Vol. 10, Issue 1 https://doi.org/10.1186/1471-2105-10-421	journal	January 2009
A genome-scale metabolic reconstruction of Pseudomonas putida KT2440: iJN746 as a cell factory Nogales, Juan; Palsson, Bernhard Ø; Thiele, Ines BMC Systems Biology, Vol. 2, Issue 1 https://doi.org/10.1186/1752-0509-2-79	journal	January 2008
Constraint-Based Modeling of Carbon Fixation and the Energetics of Electron Transfer in Geobacter metallireducens Feist, Adam M.; Nagarajan, Harish; Rotaru, Amelia-Elena PLoS Computational Biology, Vol. 10, Issue 4 https://doi.org/10.1371/journal.pcbi.1003575	journal	April 2014
RAVEN 2.0: A versatile toolbox for metabolic network reconstruction and a case study on Streptomyces coelicolor Wang, Hao; Marcišauskas, Simonas; Sánchez, Benjamín J. PLOS Computational Biology, Vol. 14, Issue 10 https://doi.org/10.1371/journal.pcbi.1006541	journal	October 2018
Flux balance analysis of the ammonia-oxidizing bacterium Nitrosomonas europaea ATCC19718 unravels specific metabolic activities while degrading toxic compounds Canto-Encalada, Gabriela; Tec-Campos, Diego; Tibocha-Bonilla, Juan D. PLOS Computational Biology, Vol. 18, Issue 2 https://doi.org/10.1371/journal.pcbi.1009828	journal	February 2022
The genome-scale metabolic model for the purple non-sulfur bacterium Rhodopseudomonas palustris Bis A53 accurately predicts phenotypes under chemoheterotrophic, chemoautotrophic, photoheterotrophic, and photoautotrophic growth conditions Tec-Campos, Diego; Posadas, Camila; Tibocha-Bonilla, Juan D. PLOS Computational Biology, Vol. 19, Issue 8 https://doi.org/10.1371/journal.pcbi.1011371	journal	August 2023
Optimization of exopolysaccharide production by response surface methodology from Enterococcus faecium isolated from the fermented foods of Western Himalaya Chauhan, Aditi; Kanwar, Sarbjit Singh International Journal of Scientific Research in Biological Sciences, Vol. 6, Issue 4 https://doi.org/10.26438/ijsrbs/v6i4.111	journal	August 2019
Bacterial exopolysaccharides: biosynthesis pathways and engineering strategies Schmid, Jochen; Sieber, Volker; Rehm, Bernd Frontiers in Microbiology, Vol. 6 https://doi.org/10.3389/fmicb.2015.00496	journal	May 2015
Thauera aminoaromatica MZ1T Identified as a Polyhydroxyalkanoate-Producing Bacterium within a Mixed Microbial Consortium Colpa, Dana I.; Zhou, Wen; Wempe, Jan Pier Bioengineering, Vol. 7, Issue 1 https://doi.org/10.3390/bioengineering7010019	journal	February 2020
Genome-Scale Metabolic Modeling Enables In-Depth Understanding of Big Data Passi, Anurag; Tibocha-Bonilla, Juan D.; Kumar, Manish Metabolites, Vol. 12, Issue 1 https://doi.org/10.3390/metabo12010014	journal	December 2021
Role of Exopolysaccharides of Pseudomonas in Heavy Metal Removal and Other Remediation Strategies Balíková, Katarína; Vojtková, Hana; Duborská, Eva Polymers, Vol. 14, Issue 20 https://doi.org/10.3390/polym14204253	journal	October 2022
Recent Advances in the Production of Exopolysaccharide (EPS) from Lactobacillus spp. and Its Application in the Food Industry: A Review Bibi, Amna; Xiong, Yongai; Rajoka, Muhammad Shahid Riaz Sustainability, Vol. 13, Issue 22 https://doi.org/10.3390/su132212429	journal	November 2021
Complete genome sequence of Thauera aminoaromatica strain MZ1T Jiang, Ke; Sanseverino, John; Chauhan, Archana Standards in Genomic Sciences, Vol. 6, Issue 3 https://doi.org/10.4056/sigs.2696029	journal	July 2012

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