Engineering and evolution of Yarrowia lipolytica for producing lipids from lignocellulosic hydrolysates

Yook, Sangdo; Deewan, Anshu; Ziolkowski, Leah; Lane, Stephan; Tohidifar, Payman; Cheng, Ming-Hsun; Singh, Vijay; Stasiewicz, Matthew J.; Rao, Christopher V.; Jin, Yong-Su

doi:10.1016/j.biortech.2024.131806

Title: Engineering and evolution of Yarrowia lipolytica for producing lipids from lignocellulosic hydrolysates

Journal Article · 12 November 2024 · Bioresource Technology

DOI: https://doi.org/10.1016/j.biortech.2024.131806 · OSTI ID:2502088

Yook, Sangdo ^[1]; Deewan, Anshu ^[1];

^[1]; Lane, Stephan ^[1]; Tohidifar, Payman ^[1]; Cheng, Ming-Hsun ^[1]; Singh, Vijay ^[1];

^[1]; Rao, Christopher V. ^[1];

^[1]

University of Illinois at Urbana-Champaign, IL (United States)

Yarrowia lipolytica, an oleaginous yeast, shows promise for industrial fermentation due to its robust acetyl-CoA flux and well-developed genetic engineering tools. However, its lack of an active xylose metabolism restricts the conversion of cellulosic sugars to valuable products. To address this, metabolic engineering, and adaptive laboratory evolution (ALE) were applied to the Y. lipolytica PO1f strain, resulting in an efficient xylose-assimilating strain (XEV). Whole-genome sequencing (WGS) of the XEV followed by reverse engineering revealed that the amplification of the heterologous oxidoreductase pathway and a mutation in the GTPase-activating protein gene (YALI0B12100g) might be the primary reasons for improved xylose assimilation in the XEV strain. When a sorghum hydrolysate was used, the XEV strain showed superior xylose consumption and lipid production compared to its parental strain (X123). This study advances our understanding of xylose metabolism in Y. lipolytica and proposes effective metabolic engineering strategies for optimizing lignocellulosic hydrolysates.

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Research Organization:: Center for Advanced Bioenergy and Bioproducts Innovation (CABBI), Urbana, IL (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: SC0018420

OSTI ID:: 2502088

Journal Information:: Bioresource Technology, Journal Name: Bioresource Technology Vol. 416; ISSN 0960-8524

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

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