The Use of Dansyl Chloride to Probe Protein Structure and Dynamics

Larson, James; Tokmina-Lukaszewska, Monika; Malone, Jadyn; Hasenoehrl, Ethan J.; Kelly, Will; Fang, Xuelan; White, Aidan; Patterson, Angela; Bothner, Brian

doi:10.3390/ijms26020456

Title: The Use of Dansyl Chloride to Probe Protein Structure and Dynamics

Journal Article · 08 January 2025 · International Journal of Molecular Sciences (Online)

DOI: https://doi.org/10.3390/ijms26020456 · OSTI ID:2497870

^[1];

^[1]; Malone, Jadyn ^[1]; Hasenoehrl, Ethan J. ^[1]; Kelly, Will ^[1]; Fang, Xuelan ^[1]; White, Aidan ^[1]; Patterson, Angela ^[2];

^[1]

Montana State University, Bozeman, MT (United States)
Indiana University, Bloomington, IN (United States)

Dansyl labeling is a widely used approach for enhancing the detection of small molecules by UV spectroscopy and mass spectrometry. It has been successfully applied to identify and quantify a variety of biological and environmental specimens. Despite clear advantages, the dansylation reaction has found very few applications in the study of proteins. We reasoned that the mild labeling conditions, small size, and rapid reaction could be beneficial for studying protein structure and dynamics. To test this, we investigated the impact of dansylation on protein fold, stability, protein–protein, and protein–cofactor interactions. We selected two model proteins, myoglobin and alcohol dehydrogenase, for analysis using native mass spectrometry and ion mobility mass spectrometry. Our work establishes the utility of dansyl chloride as a covalent probe to study protein structure and dynamics under native conditions.

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Research Organization:: Montana State University, Bozeman, MT (United States)

Sponsoring Organization:: National Institute of General Medical Sciences of the National Institutes of Health; National Science Foundation (NSF); USDOE; USDOE Office of Science (SC), Basic Energy Sciences (BES); USDOE Office of Science (SC), Basic Energy Sciences (BES). Chemical Sciences, Geosciences & Biosciences Division (CSGB)

Grant/Contract Number:: SC0018143; SC0020246

OSTI ID:: 2497870

Report Number(s):: DOE--0020246

Journal Information:: International Journal of Molecular Sciences (Online), Journal Name: International Journal of Molecular Sciences (Online) Journal Issue: 2 Vol. 26; ISSN 1422-0067

Publisher:: MDPICopyright Statement

Country of Publication:: United States

Language:: English

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37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
59 BASIC BIOLOGICAL SCIENCES
dansyl chloride
ion mobility
mass spectrometry
native mass spectrometry
protein chemistry
protein dynamics
protein labeling
protein structure

Title: The Use of Dansyl Chloride to Probe Protein Structure and Dynamics

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References (27)

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