Producing multiple chemicals through biological upcycling of waste poly(ethylene terephthalate)

Diao, Jinjin; Tian, Yuxin; Hu, Yifeng; Moon, Tae Seok

doi:10.1016/j.tibtech.2024.10.018

Title: Producing multiple chemicals through biological upcycling of waste poly(ethylene terephthalate)

Journal Article · 22 November 2024 · Trends in Biotechnology

DOI: https://doi.org/10.1016/j.tibtech.2024.10.018 · OSTI ID:2497336

Diao, Jinjin ^[1]; Tian, Yuxin ^[2]; Hu, Yifeng ^[1];

^[2]

Washington University in St Louis, MO (United States)
Washington University in St Louis, MO (United States); J. Craig Venter Institute, La Jolla, CA (United States)

Poly(ethylene terephthalate) (PET) waste is of low degradability in nature, and its mismanagement threatens numerous ecosystems. To combat the accumulation of waste PET in the biosphere, PET bio-upcycling, which integrates chemical pretreatment to produce PET-derived monomers with their microbial conversion into value-added products, has shown promise. The recently discovered Rhodococcus jostii strain PET (RPET) can metabolically degrade terephthalic acid (TPA) and ethylene glycol (EG) as sole carbon sources, and it has been developed into a microbial chassis for PET upcycling. However, the scarcity of synthetic biology tools, specifically designed for the non-model microbe RPET, limits the development of a microbial cell factory for expanding the repertoire of bioproducts from post-consumer PET. Herein, we describe the development of potent genetic tools for RPET, including (1) two inducible and titratable expression systems for tunable gene expression and (2) Serine Integrase-based Recombinational Tools (SIRT) for genome editing. Using these tools, we systematically engineer the RPET strain to ultimately establish microbial supply chains for producing multiple chemicals, including lycopene, lipids, and succinate, from post-consumer PET waste bottles, achieving the highest titer of lycopene ever reported thus far in RPET (i.e., 22.6 mg/L of lycopene, approximately 10,000-fold higher than that of the wild-type strain). Furthermore, this work highlights the great potential of plastic upcycling as a generalizable means of sustainable production of diverse chemicals.

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Research Organization:: J. Craig Venter Institute, La Jolla, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: SC0018324; SC0022003

OSTI ID:: 2497336

Journal Information:: Trends in Biotechnology, Journal Name: Trends in Biotechnology Journal Issue: 3 Vol. 43; ISSN 0167-7799

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

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36 MATERIALS SCIENCE
59 BASIC BIOLOGICAL SCIENCES
chemical bioproduction
genetic tool
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poly(ethylene terephthalate)
synthetic biology

Title: Producing multiple chemicals through biological upcycling of waste poly(ethylene terephthalate)

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