In Vivo Study of Osteochondral Defect Regeneration Using Innovative Composite Calcium Phosphate Biocement in a Sheep Model

Kresakova, Lenka; Danko, Jan; Vdoviakova, Katarina; Medvecky, Lubomir; Zert, Zdenek; Petrovova, Eva; Varga, Maros; Spakovska, Tatiana; Pribula, Jozef; Gasparek, Miroslav; Giretova, Maria; Stulajterova, Radoslava; Kolvek, Filip; Andrejcakova, Zuzana; Simaiova, Veronika; Kadasi, Marian; Vrabec, Vladimir; Toth, Teodor; Hura, Vladimir

doi:10.3390/ma14164471

Title: In Vivo Study of Osteochondral Defect Regeneration Using Innovative Composite Calcium Phosphate Biocement in a Sheep Model

Journal Article · 09 August 2021 · Materials

DOI: https://doi.org/10.3390/ma14164471 · OSTI ID:2484204

Kresakova, Lenka;

;

; Zert, Zdenek;

; Varga, Maros; Spakovska, Tatiana; Pribula, Jozef;

; Giretova, Maria; Stulajterova, Radoslava; Kolvek, Filip;

; Simaiova, Veronika; Kadasi, Marian; Vrabec, Vladimir;

; Hura, Vladimir

This study aimed to clarify the therapeutic effect and regenerative potential of the novel, amino acids-enriched acellular biocement (CAL) based on calcium phosphate on osteochondral defects in sheep. Eighteen sheep were divided into three groups, the treated group (osteochondral defects filled with a CAL biomaterial), the treated group with a biocement without amino acids (C cement), and the untreated group (spontaneous healing). Cartilages of all three groups were compared with natural cartilage (negative control). After six months, sheep were evaluated by gross appearance, histological staining, immunohistochemical staining, histological scores, X-ray, micro-CT, and MRI. Treatment of osteochondral defects by CAL resulted in efficient articular cartilage regeneration, with a predominant structural and histological characteristic of hyaline cartilage, contrary to fibrocartilage, fibrous tissue or disordered mixed tissue on untreated defect (p < 0.001, modified O’Driscoll score). MRI results of treated defects showed well-integrated and regenerated cartilage with similar signal intensity, regularity of the articular surface, and cartilage thickness with respect to adjacent native cartilage. We have demonstrated that the use of new biocement represents an effective solution for the successful treatment of osteochondral defects in a sheep animal model, can induce an endogenous regeneration of cartilage in situ, and provides several benefits for the design of future therapies supporting osteochondral defect healing.

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Sponsoring Organization:: USDOE Office of Electricity (OE), Advanced Grid Research & Development. Power Systems Engineering Research

OSTI ID:: 2484204

Journal Information:: Materials, Journal Name: Materials Journal Issue: 16 Vol. 14; ISSN MATEG9; ISSN 1996-1944

Publisher:: MDPI AGCopyright Statement

Country of Publication:: Switzerland

Language:: English

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