Proteomic Insights into Trichome Responses to Elevated Elemental Stress in Cation Exchanger (CAX) Mutants

Guo, Qi; Sarkar, Shayan; Punshon, Tracy; Tappero, Ryan; Barkla, Bronwyn J.; Hirschi, Kendal D.

doi:10.1093/pcp/pcae097

Title: Proteomic Insights into Trichome Responses to Elevated Elemental Stress in Cation Exchanger (CAX) Mutants

Journal Article · 01 September 2024 · Plant and Cell Physiology

DOI: https://doi.org/10.1093/pcp/pcae097 · OSTI ID:2483634

; Sarkar, Shayan; Punshon, Tracy; Tappero, Ryan; Barkla, Bronwyn J.;

Abstract Research on elemental distribution in plants is crucial for understanding nutrient uptake, environmental adaptation and optimizing agricultural practices for sustainable food production. Plant trichomes, with their self-contained structures and easy accessibility, offer a robust model system for investigating elemental repartitioning. Transport proteins, such as the four functional cation exchangers (CAXs) in Arabidopsis, are low-affinity, high-capacity transporters primarily located on the vacuole. Mutants in these transporters have been partially characterized, one of the phenotypes of the CAX1 mutant being altered with tolerance to low-oxygen conditions. A simple visual screen demonstrated trichome density and morphology in cax1, and quadruple CAX (cax1-4: qKO) mutants remained unaltered. Here, we used synchrotron X-ray fluorescence (SXRF) to show that trichomes in CAX-deficient lines accumulated high levels of chlorine, potassium, calcium and manganese. Proteomic analysis on isolated Arabidopsis trichomes showed changes in protein abundance in response to changes in element accumulation. The CAX mutants showed an increased abundance of plasma membrane ATPase and vacuolar H-pumping proteins, and proteins associated with water movement and endocytosis, while also showing changes in proteins associated with the regulation of plasmodesmata. These findings advance our understanding of the integration of CAX transport with elemental homeostasis within trichomes and shed light on how plants modulate protein abundance under conditions of altered elemental levels.

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Research Organization:: Brookhaven National Laboratory (BNL), Upton, NY (United States). National Synchrotron Light Source II (NSLS-II)

Sponsoring Organization:: National Institutes of Health (NIH); National Science Foundation (NSF); USDA; USDOE Office of Science (SC), Basic Energy Sciences (BES)

Grant/Contract Number:: SC0012704

OSTI ID:: 2483634

Report Number(s):: BNL--226263-2024-JAAM

Journal Information:: Plant and Cell Physiology, Journal Name: Plant and Cell Physiology Journal Issue: 12 Vol. 65; ISSN 0032-0781

Publisher:: Oxford University PressCopyright Statement

Country of Publication:: Japan

Language:: English

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Related Subjects

59 BASIC BIOLOGICAL SCIENCES
Calcium
Ionomics
Proteomics
SXRF
Transport
Trichomes
Vacuole

Title: Proteomic Insights into Trichome Responses to Elevated Elemental Stress in Cation Exchanger (CAX) Mutants

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