The promising role of proteomes and metabolomes in defining the single‐cell landscapes of plants
[1];
[2]
- Environmental Molecular Sciences Laboratory Pacific Northwest National Laboratory Richland WA 99352 USA, Plant Cell Atlas Metabolomics Committee Michigan State University East Lansing MI 48824 USA
- Department of Biological Sciences University of Alberta Edmonton AB T6G 2E9 Canada, Department of Biochemistry University of Alberta Edmonton AB T6G 2H7 Canada, Plant Cell Atlas Proteomics Committee Michigan State University East Lansing MI 48824 USA
Summary The plant community has a strong track record of RNA sequencing technology deployment, which combined with the recent advent of spatial platforms (e.g. 10× genomics) has resulted in an explosion of single‐cell and nuclei datasets that can be positioned in an in situ context within tissues (e.g. a cell atlas). In the genomics era, application of proteomics technologies in the plant sciences has always trailed behind that of RNA sequencing technologies, largely due in part to upfront cost, ease‐of‐use, and access to expertise. Conversely, the use of early analytical tools for characterizing small molecules (metabolites) from plant systems predates nucleic acid sequencing and proteomics analysis, as the search for plant‐based natural products has played a significant role in improving human health throughout history. As the plant sciences field now aims to fully define cell states, cell‐specific regulatory networks, metabolic asymmetry and phenotypes, the measurement of proteins and metabolites at the single‐cell level will be paramount. As a result of these efforts, the plant community will unlock exciting opportunities to accelerate discovery and drive toward meaningful translational outcomes.
- Sponsoring Organization:
- USDOE
- Grant/Contract Number:
- AC36-08GO28308
- OSTI ID:
- 2479824
- Journal Information:
- New Phytologist, Journal Name: New Phytologist Journal Issue: 3 Vol. 245; ISSN 0028-646X
- Publisher:
- Wiley-BlackwellCopyright Statement
- Country of Publication:
- United Kingdom
- Language:
- English
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