Rapid curing dynamics of PEG-thiol-ene resins allow facile 3D bioprinting and in-air cell-laden microgel fabrication

Jang, Lindy K.; Ahlquist, Jesse T.; Ye, Congwang; Trujillo, Juliana; Triplett, Michael; Moya, Monica L.; Robertson, Claire; Hynes, William; Wasson, Elisa M.

doi:10.1088/1748-605x/ad8540

Title: Rapid curing dynamics of PEG-thiol-ene resins allow facile 3D bioprinting and in-air cell-laden microgel fabrication

Journal Article · 25 November 2024 · Biomedical Materials

DOI: https://doi.org/10.1088/1748-605x/ad8540 · OSTI ID:2479488

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^[1]; Ye, Congwang ^[1]; Trujillo, Juliana ^[2];

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Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)
University of Virginia, Charlottesville, VA (United States)
Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States); Los Alamos National Laboratory (LANL), Los Alamos, NM (United States)

Thiol-norbornene photoclick hydrogels are highly efficient in tissue engineering applications due to their fast gelation, cytocompatibility, and tunability. In this work, we utilized the advantageous features of polyethylene glycol (PEG)-thiol-ene resins to enable fabrication of complex and heterogeneous tissue scaffolds using 3D bioprinting and in-air drop encapsulation techniques. We demonstrated that photoclickable PEG-thiol-ene resins could be tuned by varying the ratio of PEG-dithiol to PEG norbornene to generate a wide range of mechanical stiffness (0.5–12 kPa) and swelling ratios. Importantly, all formulations maintained a constant, rapid gelation time (<0.5 s). We used this resin in biological projection microstereolithography (BioPµSL) to print complex structures with geometric fidelity and demonstrated biocompatibility by printing cell-laden microgrids. Moreover, the rapid gelling kinetics of this resin permitted high-throughput fabrication of tunable, cell-laden microgels in air using a biological in-air drop encapsulation apparatus (BioIDEA). We demonstrated that these microgels could support cell viability and be assembled into a gradient structure. This PEG-thiol-ene resin, along with BioPµSL and BioIDEA technology, will allow rapid fabrication of complex and heterogeneous tissues that mimic native tissues with cellular and mechanical gradients. The engineered tissue scaffolds with a controlled microscale porosity could be utilized in applications including gradient tissue engineering, biosensing, and in vitro tissue models.

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Research Organization:: Los Alamos National Laboratory (LANL), Los Alamos, NM (United States); Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)

Sponsoring Organization:: USDOE Laboratory Directed Research and Development (LDRD) Program; USDOE National Nuclear Security Administration (NNSA)

Grant/Contract Number:: 89233218CNA000001; AC52-07NA27344

OSTI ID:: 2479488

Report Number(s):: LA-UR--24-25864; LLNL-JRNL-861494

Journal Information:: Biomedical Materials, Journal Name: Biomedical Materials Journal Issue: 1 Vol. 20; ISSN 1748-6041

Publisher:: IOP PublishingCopyright Statement

Country of Publication:: United States

Language:: English

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Title: Rapid curing dynamics of PEG-thiol-ene resins allow facile 3D bioprinting and in-air cell-laden microgel fabrication

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