Label-Free Anti-Brownian Trapping of Single Nanoparticles in Solution

Carpenter, William B.; Lavania, Abhijit A.; Squires, Allison H.; Moerner, W. E.

doi:10.1021/acs.jpcc.4c05878

Title: Label-Free Anti-Brownian Trapping of Single Nanoparticles in Solution

Journal Article · 18 November 2024 · Journal of Physical Chemistry. C

DOI: https://doi.org/10.1021/acs.jpcc.4c05878 · OSTI ID:2478040

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Stanford University, CA (United States); The Ohio State University, Columbus, OH (United States)
Stanford University, CA (United States); Double Helix Optics, Boulder, CO (United States)
University of Chicago, IL (United States)
Stanford University, CA (United States)

Today, biomolecular nanoparticles are prevalent as diagnostic tools and molecular delivery carriers, and it is particularly useful to examine individuals within a sample population to quantify the variations between objects and directly observe the molecular dynamics involving these objects. Using interferometric scattering as a highly sensitive label-free detection scheme, we recently developed the interferometric scattering anti-Brownian electrokinetic (ISABEL) trap to hold a single nanoparticle in solution for extended optical observation. In this perspective, we describe how we implemented this trap, how it extends the capabilities of previous ABEL traps, and how we have begun to study individual carboxysomes, a fascinating biological carbon fixation nanocompartment. By monitoring single nanocompartments for seconds to minutes in the ISABEL trap using simultaneous interferometric scattering and fluorescence spectroscopy, we have demonstrated single-compartment mass measurements, cargo-loading trends, and redox sensing inside individual particles. These experiments benefit from rich multiplexed correlative measurements utilizing both scattering and fluorescence with many exciting future capabilities within reach.

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Research Organization:: Stanford University, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES); USDOE Office of Science (SC), Basic Energy Sciences (BES). Chemical Sciences, Geosciences & Biosciences Division (CSGB)

Grant/Contract Number:: FG02-07ER15892; SC0025385

OSTI ID:: 2478040

Journal Information:: Journal of Physical Chemistry. C, Journal Name: Journal of Physical Chemistry. C Journal Issue: 47 Vol. 128; ISSN 1932-7447

Publisher:: American Chemical SocietyCopyright Statement

Country of Publication:: United States

Language:: English

References (78)

Label‐free discrimination of extracellular vesicles from large lipoproteins Kashkanova, Anna D.; Blessing, Martin; Reischke, Marie Journal of Extracellular Vesicles, Vol. 12, Issue 8 https://doi.org/10.1002/jev2.12348	journal	July 2023
Zeta potential: a case study of cationic, anionic, and neutral liposomes Smith, Mackensie C.; Crist, Rachael M.; Clogston, Jeffrey D. Analytical and Bioanalytical Chemistry, Vol. 409, Issue 24 https://doi.org/10.1007/s00216-017-0527-z	journal	July 2017
Optimal strategy for trapping single fluorescent molecules in solution using the ABEL trap Wang, Q.; Moerner, W. E. Applied Physics B, Vol. 99, Issue 1-2 https://doi.org/10.1007/s00340-009-3843-y	journal	December 2009
Critical Evaluation of Nanoparticle Tracking Analysis (NTA) by NanoSight for the Measurement of Nanoparticles and Protein Aggregates Filipe, Vasco; Hawe, Andrea; Jiskoot, Wim Pharmaceutical Research, Vol. 27, Issue 5 https://doi.org/10.1007/s11095-010-0073-2	journal	March 2010
Evaluation of nanoparticle tracking analysis (NTA) in the characterization of therapeutic antibodies and seasonal influenza vaccines: pros and cons Patois, E.; Capelle, M. A. H.; Palais, C. Journal of Drug Delivery Science and Technology, Vol. 22, Issue 5 https://doi.org/10.1016/S1773-2247(12)50069-9	journal	January 2012
LED-based Interferometric Reflectance Imaging Sensor for quantitative dynamic monitoring of biomolecular interactions Daaboul, G. G.; Vedula, R. S.; Ahn, S. Biosensors and Bioelectronics, Vol. 26, Issue 5 https://doi.org/10.1016/j.bios.2010.09.038	journal	January 2011
Protein Oligomerization Monitored by Fluorescence Fluctuation Spectroscopy: Self-Assembly of Rubisco Activase Chakraborty, Manas; Kuriata, Agnieszka M.; Nathan Henderson, J. Biophysical Journal, Vol. 103, Issue 5 https://doi.org/10.1016/j.bpj.2012.07.034	journal	September 2012
Single-molecule trapping and measurement in solution Bespalova, Maria I.; Mahanta, Sushanta; Krishnan, Madhavi Current Opinion in Chemical Biology, Vol. 51 https://doi.org/10.1016/j.cbpa.2019.05.013	journal	August 2019
Characterising biomolecular interactions and dynamics with mass photometry Asor, Roi; Kukura, Philipp Current Opinion in Chemical Biology, Vol. 68 https://doi.org/10.1016/j.cbpa.2022.102132	journal	June 2022
Conserved and repetitive motifs in an intrinsically disordered protein drive ⍺-carboxysome assembly Turnšek, Julia B.; Oltrogge, Luke M.; Savage, David F. Journal of Biological Chemistry, Vol. 300, Issue 8 https://doi.org/10.1016/j.jbc.2024.107532	journal	August 2024
Structure of Halothiobacillus neapolitanus Carboxysomes by Cryo-electron Tomography Schmid, Michael F.; Paredes, Angel M.; Khant, Htet A. Journal of Molecular Biology, Vol. 364, Issue 3 https://doi.org/10.1016/j.jmb.2006.09.024	journal	December 2006
New discoveries expand possibilities for carboxysome engineering Borden, Julia S.; Savage, David F. Current Opinion in Microbiology, Vol. 61 https://doi.org/10.1016/j.mib.2021.03.002	journal	June 2021
Applications of particle-tracking analysis to the determination of size distributions and concentrations of nanoparticles in environmental, biological and food samples Gallego-Urrea, Julian A.; Tuoriniemi, Jani; Hassellöv, Martin TrAC Trends in Analytical Chemistry, Vol. 30, Issue 3 https://doi.org/10.1016/j.trac.2011.01.005	journal	March 2011
Convex Lens-Induced Confinement for Imaging Single Molecules Leslie, Sabrina R.; Fields, Alexander P.; Cohen, Adam E. Analytical Chemistry, Vol. 82, Issue 14 https://doi.org/10.1021/ac101041s	journal	July 2010
Development of Spectral Nano-Flow Cytometry for High-Throughput Multiparameter Analysis of Individual Biological Nanoparticles Li, Lihong; Wang, Shuo; Xue, Junwei Analytical Chemistry, Vol. 95, Issue 6 https://doi.org/10.1021/acs.analchem.2c05159	journal	February 2023
Probing the Internal pH and Permeability of a Carboxysome Shell Huang, Jiafeng; Jiang, Qiuyao; Yang, Mengru Biomacromolecules, Vol. 23, Issue 10 https://doi.org/10.1021/acs.biomac.2c00781	journal	September 2022
Metal Catalysts for Heterogeneous Catalysis: From Single Atoms to Nanoclusters and Nanoparticles Liu, Lichen; Corma, Avelino Chemical Reviews, Vol. 118, Issue 10 https://doi.org/10.1021/acs.chemrev.7b00776	journal	April 2018
Genetically Encoded Fluorescent Biosensors Illuminate the Spatiotemporal Regulation of Signaling Networks Greenwald, Eric C.; Mehta, Sohum; Zhang, Jin Chemical Reviews, Vol. 118, Issue 24 https://doi.org/10.1021/acs.chemrev.8b00333	journal	December 2018
Exploring Masses and Internal Mass Distributions of Single Carboxysomes in Free Solution Using Fluorescence and Interferometric Scattering in an Anti-Brownian Trap Lavania, Abhijit A.; Carpenter, William B.; Oltrogge, Luke M. The Journal of Physical Chemistry B, Vol. 126, Issue 43 https://doi.org/10.1021/acs.jpcb.2c05939	journal	October 2022
Fluorescence-Combined Interferometric Scattering Imaging Reveals Nanoscale Dynamic Events of Single Nascent Adhesions in Living Cells Park, Jin-Sung; Lee, Il-Buem; Moon, Hyeon-Min The Journal of Physical Chemistry Letters, Vol. 11, Issue 23 https://doi.org/10.1021/acs.jpclett.0c02103	journal	November 2020
Ratiometric Sensing of Redox Environments Inside Individual Carboxysomes Trapped in Solution Carpenter, William B.; Lavania, Abhijit A.; Borden, Julia S. The Journal of Physical Chemistry Letters, Vol. 13, Issue 20 https://doi.org/10.1021/acs.jpclett.2c00782	journal	May 2022
Weighing the DNA Content of Adeno-Associated Virus Vectors with Zeptogram Precision Using Nanomechanical Resonators Katsikis, Georgios; Hwang, Iris E.; Wang, Wade Nano Letters, Vol. 22, Issue 4 https://doi.org/10.1021/acs.nanolett.1c04092	journal	February 2022
Dual-Angle Interferometric Scattering Microscopy for Optical Multiparametric Particle Characterization Olsén, Erik; García Rodríguez, Berenice; Skärberg, Fredrik Nano Letters, Vol. 24, Issue 6 https://doi.org/10.1021/acs.nanolett.3c03539	journal	January 2024
Interferometric Scattering Enables Fluorescence-Free Electrokinetic Trapping of Single Nanoparticles in Free Solution Squires, Allison H.; Lavania, Abhijit A.; Dahlberg, Peter D. Nano Letters, Vol. 19, Issue 6 https://doi.org/10.1021/acs.nanolett.9b01514	journal	May 2019
Interferometric Scattering Microscopy: Seeing Single Nanoparticles and Molecules via Rayleigh Scattering Taylor, Richard W.; Sandoghdar, Vahid Nano Letters, Vol. 19, Issue 8 https://doi.org/10.1021/acs.nanolett.9b01822	journal	July 2019
Nanoparticle Manufacturing – Heterogeneity through Processes to Products Stavis, Samuel M.; Fagan, Jeffrey A.; Stopa, Michael ACS Applied Nano Materials, Vol. 1, Issue 9 https://doi.org/10.1021/acsanm.8b01239	journal	August 2018
Simultaneous, Single-Particle Measurements of Size and Loading Give Insights into the Structure of Drug-Delivery Nanoparticles Kamanzi, Albert; Gu, Yifei; Tahvildari, Radin ACS Nano, Vol. 15, Issue 12 https://doi.org/10.1021/acsnano.1c04862	journal	November 2021
Quantitative Visualization of Lipid Nanoparticle Fusion as a Function of Formulation and Process Parameters Kamanzi, Albert; Zhang, Yao; Gu, Yifei ACS Nano, Vol. 18, Issue 28 https://doi.org/10.1021/acsnano.3c12981	journal	July 2024
Combined Scattering, Interferometric, and Fluorescence Oblique Illumination for Live Cell Nanoscale Imaging Zheng, Yujie; Lim, Yean Jin; Lin, Hanqi ACS Photonics, Vol. 9, Issue 12 https://doi.org/10.1021/acsphotonics.2c01143	journal	November 2022
Lifetime and Spectrally Resolved Characterization of the Photodynamics of Single Fluorophores in Solution Using the Anti-Brownian Electrokinetic Trap Wang, Quan; Moerner, W. E. The Journal of Physical Chemistry B, Vol. 117, Issue 16 https://doi.org/10.1021/jp308949d	journal	November 2012
Tracking Single Particles on Supported Lipid Membranes: Multimobility Diffusion and Nanoscopic Confinement Hsieh, Chia-Lung; Spindler, Susann; Ehrig, Jens The Journal of Physical Chemistry B, Vol. 118, Issue 6 https://doi.org/10.1021/jp412203t	journal	January 2014
Optical Trapping of 12 nm Dielectric Spheres Using Double-Nanoholes in a Gold Film Pang, Yuanjie; Gordon, Reuven Nano Letters, Vol. 11, Issue 9 https://doi.org/10.1021/nl201807z	journal	September 2011
Optically Controlled Thermophoretic Trapping of Single Nano-Objects Braun, Marco; Cichos, Frank ACS Nano, Vol. 7, Issue 12 https://doi.org/10.1021/nn404980k	journal	November 2013
Single-molecule motions enable direct visualization of biomolecular interactions in solution Wang, Quan; Moerner, W. E. Nature Methods, Vol. 11, Issue 5 https://doi.org/10.1038/nmeth.2882	journal	March 2014
Optical detection of single non-absorbing molecules using the surface plasmon resonance of a gold nanorod Zijlstra, Peter; Paulo, Pedro M. R.; Orrit, Michel Nature Nanotechnology, Vol. 7, Issue 6 https://doi.org/10.1038/nnano.2012.51	journal	April 2012
Multi-resolution 3D visualization of the early stages of cellular uptake of peptide-coated nanoparticles Welsher, Kevin; Yang, Haw Nature Nanotechnology, Vol. 9, Issue 3 https://doi.org/10.1038/nnano.2014.12	journal	February 2014
Single-molecule electrometry Ruggeri, Francesca; Zosel, Franziska; Mutter, Natalie Nature Nanotechnology, Vol. 12, Issue 5 https://doi.org/10.1038/nnano.2017.26	journal	March 2017
Self-induced back-action optical trapping of dielectric nanoparticles Juan, Mathieu L.; Gordon, Reuven; Pang, Yuanjie Nature Physics, Vol. 5, Issue 12 https://doi.org/10.1038/nphys1422	journal	October 2009
Electric-field sensing using single diamond spins Dolde, F.; Fedder, H.; Doherty, M. W. Nature Physics, Vol. 7, Issue 6 https://doi.org/10.1038/nphys1969	journal	April 2011
Interferometric scattering microscopy and its combination with single-molecule fluorescence imaging Ortega Arroyo, Jaime; Cole, Daniel; Kukura, Philipp Nature Protocols, Vol. 11, Issue 4 https://doi.org/10.1038/nprot.2016.022	journal	March 2016
Single-particle mass spectrometry with arrays of frequency-addressed nanomechanical resonators Sage, Eric; Sansa, Marc; Fostner, Shawn Nature Communications, Vol. 9, Issue 1 https://doi.org/10.1038/s41467-018-05783-4	journal	August 2018
Single-molecule trapping and spectroscopy reveals photophysical heterogeneity of phycobilisomes quenched by Orange Carotenoid Protein Squires, Allison H.; Dahlberg, Peter D.; Liu, Haijun Nature Communications, Vol. 10, Issue 1 https://doi.org/10.1038/s41467-019-09084-2	journal	March 2019
Naturally-occurring cholesterol analogues in lipid nanoparticles induce polymorphic shape and enhance intracellular delivery of mRNA Patel, Siddharth; Ashwanikumar, N.; Robinson, Ema Nature Communications, Vol. 11, Issue 1 https://doi.org/10.1038/s41467-020-14527-2	journal	February 2020
Real-time 3D single molecule tracking Hou, Shangguo; Exell, Jack; Welsher, Kevin Nature Communications, Vol. 11, Issue 1 https://doi.org/10.1038/s41467-020-17444-6	journal	July 2020
Rubisco forms a lattice inside alpha-carboxysomes Metskas, Lauren Ann; Ortega, Davi; Oltrogge, Luke M. Nature Communications, Vol. 13, Issue 1 https://doi.org/10.1038/s41467-022-32584-7	journal	August 2022
Confocal interferometric scattering microscopy reveals 3D nanoscopic structure and dynamics in live cells Küppers, Michelle; Albrecht, David; Kashkanova, Anna D. Nature Communications, Vol. 14, Issue 1 https://doi.org/10.1038/s41467-023-37497-7	journal	April 2023
Single-molecule fluorescence multiplexing by multi-parameter spectroscopic detection of nanostructured FRET labels Chu, Jiachong; Ejaz, Ayesha; Lin, Kyle M. Nature Nanotechnology, Vol. 19, Issue 8 https://doi.org/10.1038/s41565-024-01672-8	journal	May 2024
Engineering precision nanoparticles for drug delivery Mitchell, Michael J.; Billingsley, Margaret M.; Haley, Rebecca M. Nature Reviews Drug Discovery, Vol. 20, Issue 2 https://doi.org/10.1038/s41573-020-0090-8	journal	December 2020
ABEL-FRET: tether-free single-molecule FRET with hydrodynamic profiling Wilson, Hugh; Wang, Quan Nature Methods, Vol. 18, Issue 7 https://doi.org/10.1038/s41592-021-01173-9	journal	June 2021
Mass-sensitive particle tracking to elucidate the membrane-associated MinDE reaction cycle Heermann, Tamara; Steiert, Frederik; Ramm, Beatrice Nature Methods, Vol. 18, Issue 10 https://doi.org/10.1038/s41592-021-01260-x	journal	October 2021
Mass photometry enables label-free tracking and mass measurement of single proteins on lipid bilayers Foley, Eric D. B.; Kushwah, Manish S.; Young, Gavin Nature Methods, Vol. 18, Issue 10 https://doi.org/10.1038/s41592-021-01261-w	journal	October 2021
Precision size and refractive index analysis of weakly scattering nanoparticles in polydispersions Kashkanova, Anna D.; Blessing, Martin; Gemeinhardt, André Nature Methods, Vol. 19, Issue 5 https://doi.org/10.1038/s41592-022-01460-z	journal	May 2022
Label-free nanofluidic scattering microscopy of size and mass of single diffusing molecules and nanoparticles Špačková, Barbora; Klein Moberg, Henrik; Fritzsche, Joachim Nature Methods, Vol. 19, Issue 6 https://doi.org/10.1038/s41592-022-01491-6	journal	May 2022
Capturing the start point of the virus–cell interaction with high-speed 3D single-virus tracking Johnson, Courtney; Exell, Jack; Lin, Yuxin Nature Methods, Vol. 19, Issue 12 https://doi.org/10.1038/s41592-022-01672-3	journal	November 2022
Nanoscopic substructures of raft-mimetic liquid-ordered membrane domains revealed by high-speed single-particle tracking Wu, Hsiao-Mei; Lin, Ying-Hsiu; Yen, Tzu-Chi Scientific Reports, Vol. 6, Issue 1 https://doi.org/10.1038/srep20542	journal	February 2016
Methods of single-molecule fluorescence spectroscopy and microscopy Moerner, W. E.; Fromm, David P. Review of Scientific Instruments, Vol. 74, Issue 8 https://doi.org/10.1063/1.1589587	journal	August 2003
Single-molecule diffusometry reveals the nucleotide-dependent oligomerization pathways of Nicotiana tabacum Rubisco activase Wang, Quan; Serban, Andrew J.; Wachter, Rebekka M. The Journal of Chemical Physics, Vol. 148, Issue 12 https://doi.org/10.1063/1.5005930	journal	March 2018
A bottom-up perspective on photodynamics and photoprotection in light-harvesting complexes using anti-Brownian trapping Squires, Allison H.; Wang, Quan; Dahlberg, Peter D. The Journal of Chemical Physics, Vol. 156, Issue 7 https://doi.org/10.1063/5.0079042	journal	February 2022
Suppressing Brownian motion of individual biomolecules in solution Cohen, A. E.; Moerner, W. E. Proceedings of the National Academy of Sciences, Vol. 103, Issue 12 https://doi.org/10.1073/pnas.0509976103	journal	March 2006
Principal-components analysis of shape fluctuations of single DNA molecules Cohen, A. E.; Moerner, W. E. Proceedings of the National Academy of Sciences, Vol. 104, Issue 31 https://doi.org/10.1073/pnas.0610396104	journal	May 2007
Electrokinetic trapping at the one nanometer limit Fields, A. P.; Cohen, A. E. Proceedings of the National Academy of Sciences, Vol. 108, Issue 22 https://doi.org/10.1073/pnas.1103554108	journal	May 2011
Modularity of a carbon-fixing protein organelle Bonacci, W.; Teng, P. K.; Afonso, B. Proceedings of the National Academy of Sciences, Vol. 109, Issue 2 https://doi.org/10.1073/pnas.1108557109	journal	December 2011
Sensing cooperativity in ATP hydrolysis for single multisubunit enzymes in solution Jiang, Y.; Douglas, N. R.; Conley, N. R. Proceedings of the National Academy of Sciences, Vol. 108, Issue 41 https://doi.org/10.1073/pnas.1112244108	journal	September 2011
Single-molecule spectroscopy reveals photosynthetic LH2 complexes switch between emissive states Schlau-Cohen, G. S.; Wang, Q.; Southall, J. Proceedings of the National Academy of Sciences, Vol. 110, Issue 27 https://doi.org/10.1073/pnas.1310222110	journal	June 2013
Dissecting pigment architecture of individual photosynthetic antenna complexes in solution Wang, Quan; Moerner, W. E. Proceedings of the National Academy of Sciences, Vol. 112, Issue 45 https://doi.org/10.1073/pnas.1514027112	journal	October 2015
Direct single-molecule measurements of phycocyanobilin photophysics in monomeric C-phycocyanin Squires, Allison H.; Moerner, W. E. Proceedings of the National Academy of Sciences, Vol. 114, Issue 37 https://doi.org/10.1073/pnas.1705435114	journal	August 2017
Toward a glycyl radical enzyme containing synthetic bacterial microcompartment to produce pyruvate from formate and acetate Kirst, Henning; Ferlez, Bryan H.; Lindner, Steffen N. Proceedings of the National Academy of Sciences, Vol. 119, Issue 8 https://doi.org/10.1073/pnas.2116871119	journal	February 2022
Investigating Mitochondrial Redox Potential with Redox-sensitive Green Fluorescent Protein Indicators Hanson, George T.; Aggeler, Robert; Oglesbee, Devin Journal of Biological Chemistry, Vol. 279, Issue 13 https://doi.org/10.1074/jbc.M312846200	journal	January 2004
Diamond-based single-photon emitters Aharonovich, I.; Castelletto, S.; Simpson, D. A. Reports on Progress in Physics, Vol. 74, Issue 7 https://doi.org/10.1088/0034-4885/74/7/076501	journal	June 2011
Detection and Spectroscopy of Gold Nanoparticles Using Supercontinuum White Light Confocal Microscopy Lindfors, K.; Kalkbrenner, T.; Stoller, P. Physical Review Letters, Vol. 93, Issue 3 https://doi.org/10.1103/PhysRevLett.93.037401	journal	July 2004
Re-engineering redox-sensitive green fluorescent protein for improved response rate Cannon, M. B. Protein Science, Vol. 15, Issue 1 https://doi.org/10.1110/ps.051734306	journal	January 2006
Quantitative mass imaging of single biological macromolecules Young, Gavin; Hundt, Nikolas; Cole, Daniel Science, Vol. 360, Issue 6387 https://doi.org/10.1126/science.aar5839	journal	April 2018
Characterization of the Carboxysomal Carbonic Anhydrase CsoSCA from Halothiobacillus neapolitanus Heinhorst, S.; Williams, E. B.; Cai, F. Journal of Bacteriology, Vol. 188, Issue 23 https://doi.org/10.1128/JB.00990-06	journal	September 2006
Interferometric Scattering Microscopy Young, Gavin; Kukura, Philipp Annual Review of Physical Chemistry, Vol. 70, Issue 1 https://doi.org/10.1146/annurev-physchem-050317-021247	journal	June 2019
iSCAT microscopy and particle tracking with tailored spatial coherence Mazaheri, Mahdi; Kasaian, Kiarash; Albrecht, David Optica, Vol. 11, Issue 7 https://doi.org/10.1364/OPTICA.523788	journal	July 2024
The Pentameric Vertex Proteins Are Necessary for the Icosahedral Carboxysome Shell to Function as a CO2 Leakage Barrier Cai, Fei; Menon, Balaraj B.; Cannon, Gordon C. PLoS ONE, Vol. 4, Issue 10 https://doi.org/10.1371/journal.pone.0007521	journal	October 2009
The Bacterial Carbon-Fixing Organelle Is Formed by Shell Envelopment of Preassembled Cargo Chen, Anna H.; Robinson-Mosher, Avi; Savage, David F. PLoS ONE, Vol. 8, Issue 9 https://doi.org/10.1371/journal.pone.0076127	journal	September 2013
A Comprehensive Review of Fluorescence Correlation Spectroscopy Yu, Lan; Lei, Yunze; Ma, Ying Frontiers in Physics, Vol. 9 https://doi.org/10.3389/fphy.2021.644450	journal	April 2021

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37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
Fluorescence
Fluorescence detection
Nanoparticles
Redox reactions
Scattering

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