Induced and natural variation affect traits independently in hybrid Populus

Guo, Weier; Bastiaanse, Héloïse; Maloof, Julin N.; Comai, Luca; Henry, Isabelle M.; de Koning, ed., D-J

doi:10.1093/g3journal/jkae218

Title: Induced and natural variation affect traits independently in hybrid Populus

Journal Article · 11 September 2024 · G3

DOI: https://doi.org/10.1093/g3journal/jkae218 · OSTI ID:2476416

;

; de Koning, ed., D-J

Abstract The genetic control of many plant traits can be highly complex. Both allelic variation (sequence change) and dosage variation (copy number change) contribute to a plant's phenotype. While numerous studies have investigated the effect of allelic or dosage variation, very few have documented both within the same system, leaving their relative contribution to phenotypic effects unclear. The Populus genome is highly polymorphic, and poplars are fairly tolerant of gene dosage variation. Here, using a previously established Populus hybrid F1 population, we assessed and compared the effect of natural allelic variation and induced dosage variation on biomass, phenology, and leaf morphology traits. We identified QTLs for many of these traits, but our results indicate limited overlap between the QTLs associated with natural allelic variation and induced dosage variation. Additionally, the integration of data from both allelic and dosage variation identifies a larger set of QTLs that together explain a larger percentage of the phenotypic variance. Finally, our results suggest that the effect of the large indels might mask that of allelic QTLs. Our study helps clarify the relationship between allelic and dosage variation and their effects on quantitative traits.

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Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: SC0007183

OSTI ID:: 2476416

Journal Information:: G3, Journal Name: G3 Journal Issue: 11 Vol. 14; ISSN 2160-1836

Publisher:: Oxford University PressCopyright Statement

Country of Publication:: United States

Language:: English

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