Stress-regulated Arabidopsis GAT2 is a low affinity γ-aminobutyric acid transporter

Meier, Stefan; Bautzmann, Robin; Komarova, Nataliya Y.; Ernst, Viona; Suter Grotemeyer, Marianne; Schröder, Kirsten; Haindrich, Alexander C.; Vega Fernández, Adriana; Robert, Christelle A. M.; Ward, John M.; Rentsch, Doris; Murphy, ed., Angus

doi:10.1093/jxb/erae321

Title: Stress-regulated Arabidopsis GAT2 is a low affinity γ-aminobutyric acid transporter

Journal Article · 25 July 2024 · Journal of Experimental Botany

DOI: https://doi.org/10.1093/jxb/erae321 · OSTI ID:2475883

Meier, Stefan; Bautzmann, Robin; Komarova, Nataliya Y.; Ernst, Viona;

; Schröder, Kirsten;

; Vega Fernández, Adriana;

; Ward, John M.;

; Murphy, ed., Angus

Abstract The four-carbon non-proteinogenic amino acid γ-aminobutyric acid (GABA) accumulates to high levels in plants in response to various abiotic and biotic stress stimuli, and plays a role in C:N balance, signaling, and as a transport regulator. Expression in Xenopus oocytes and voltage-clamping allowed the characterization of Arabidopsis GAT2 (At5g41800) as a low affinity GABA transporter with a K0.5GABA ~8 mM. l-Alanine and butylamine represented additional substrates. GABA-induced currents were strongly dependent on the membrane potential, reaching the highest affinity and highest transport rates at strongly negative membrane potentials. Mutation of Ser17, previously reported to be phosphorylated in planta, did not result in altered affinity. In a short-term stress experiment, AtGAT2 mRNA levels were up-regulated at low water potential and under osmotic stress (polyethylene glycol and mannitol). Furthermore, AtGAT2 promoter activity was detected in vascular tissues, maturating pollen, and the phloem unloading region of young seeds. Even though this suggested a role for AtGAT2 in long-distance transport and loading of sink organs, under the conditions tested neither AtGAT2-overexpressing plants, atgat2 or atgat1 T-DNA insertion lines, nor atgat1 atgat2 doubleknockout mutants differed from wild-type plants in growth on GABA, amino acid levels, or resistance to salt and osmotic stress.

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Sponsoring Organization:: USDOE

Grant/Contract Number:: FG02-07ER15886

OSTI ID:: 2475883

Journal Information:: Journal of Experimental Botany, Journal Name: Journal of Experimental Botany Journal Issue: 20 Vol. 75; ISSN 0022-0957

Publisher:: Oxford University PressCopyright Statement

Country of Publication:: United Kingdom

Language:: English

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Auxin efflux by PIN-FORMED proteins is activated by two different protein kinases, D6 PROTEIN KINASE and PINOID Zourelidou, Melina; Absmanner, Birgit; Weller, Benjamin eLife, Vol. 3 https://doi.org/10.7554/eLife.02860	journal	June 2014

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