The Advantages of Combining Morphological and Molecular Methods to Characterise Zooplankton Communities: A Case Study of the UNESCO Biosphere Reserve of the Berlengas Archipelago, Portugal

Simões, Marco; Cotrim Marques, Sónia; Costa, Cátia; da Luz Calado, Maria; Lobo-Arteaga, Jorge; Bartilotti, Cátia; Jorge Campos, Maria; Leandro, Sérgio Miguel; Antunes, Agostinho

doi:10.3390/oceans5040046

Title: The Advantages of Combining Morphological and Molecular Methods to Characterise Zooplankton Communities: A Case Study of the UNESCO Biosphere Reserve of the Berlengas Archipelago, Portugal

Journal Article · 26 October 2024 · Oceans

DOI: https://doi.org/10.3390/oceans5040046 · OSTI ID:2475237

;

; da Luz Calado, Maria;

;

; Leandro, Sérgio Miguel;

Understanding the ecological dynamics of zooplankton communities is crucial to precisely assessing the health of marine ecosystems and their management. Metabarcoding has contributed to a better understanding of biodiversity in marine environments. However, this methodology still requires protocol optimisation. Here, we used a complementary approach combining molecular and morphological identification methods to identify the zooplankton community inhabiting the Berlengas Archipelago, Portugal. The presence of non-indigenous species was also assessed. The results showed that the metabarcoding approach outperformed the classical morphological identification method, detecting more species with higher resolution. Nevertheless, the classical method was able to identify species that were not detected by the molecular approaches, probably due to a lack of reference data in the databases. The comparison between different molecular approaches showed that COI and bulk DNA gave better results than 18S rRNA and eDNA by detecting higher species diversity. However, complementarities were observed between them. Molecular tools also proved effective in identifying several potential non-indigenous species, identifying, for the first time, several potentially unreported NIS inhabiting the Portuguese marine ecosystems. Overall, our results confirmed the importance of combining both classical and molecular methods to obtain a more refined assessment of the zooplanktonic communities in marine environments.

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Sponsoring Organization:: USDOE Office of Energy Efficiency and Renewable Energy (EERE), Transportation Office. Fuel Cell Technologies Office

OSTI ID:: 2475237

Journal Information:: Oceans, Journal Name: Oceans Journal Issue: 4 Vol. 5; ISSN OBSCAL; ISSN 2673-1924

Publisher:: MDPI AGCopyright Statement

Country of Publication:: Country unknown/Code not available

Language:: English

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Title: The Advantages of Combining Morphological and Molecular Methods to Characterise Zooplankton Communities: A Case Study of the UNESCO Biosphere Reserve of the Berlengas Archipelago, Portugal

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