The DYRKP1 kinase regulates cell wall degradation in Chlamydomonas by inducing matrix metalloproteinase expression

Kim, Minjae; Jorge, Gabriel Lemes; Aschern, Moritz; Cuiné, Stéphan; Bertrand, Marie; Mekhalfi, Malika; Putaux, Jean-Luc; Yang, Jae-Seong; Thelen, Jay J.; Beisson, Fred; Peltier, Gilles; Li-Beisson, Yonghua

doi:10.1093/plcell/koae271

Title: The DYRKP1 kinase regulates cell wall degradation in Chlamydomonas by inducing matrix metalloproteinase expression

Journal Article · 14 October 2024 · The Plant Cell

DOI: https://doi.org/10.1093/plcell/koae271 · OSTI ID:2472963

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Abstract The cell wall of plants and algae is an important cell structure that protects cells from changes in the external physical and chemical environment. This extracellular matrix, composed of polysaccharides and glycoproteins, must be constantly remodeled throughout the life cycle. However, compared to matrix polysaccharides, little is known about the mechanisms regulating the formation and degradation of matrix glycoproteins. We report here that a plant kinase belonging to the dual-specificity tyrosine phosphorylation-regulated kinase (DYRKP1) family present in all eukaryotes regulates cell wall degradation after mitosis of Chlamydomonas reinhardtii by inducing the expression of matrix metalloproteinases. Without DYRKP1, daughter cells cannot disassemble parental cell walls and remain trapped inside for more than 10 days. On the other hand, the dual-specificity tyrosine phosphorylation-regulated kinase complementation lines show normal degradation of the parental cell wall. Transcriptomic and proteomic analyses indicate a marked downregulation of MMP gene expression and accumulation, respectively, in the dyrkp1 mutants. The mutants deficient in matrix metalloproteinases retain palmelloid structures for a longer time than the background strain, like dyrkp1 mutants. Our findings show that dual-specificity tyrosine phosphorylation-regulated kinase, by ensuring timely MMP expression, enables the successful execution of the cell cycle. Altogether, this study provides insight into the life cycle regulation in plants and algae.

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Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: SC0023142

OSTI ID:: 2472963

Journal Information:: The Plant Cell, Journal Name: The Plant Cell; ISSN 1040-4651

Publisher:: Oxford University PressCopyright Statement

Country of Publication:: United States

Language:: English

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