A maize semi-dwarf mutant reveals a GRAS transcription factor involved in brassinosteroid signaling

Kaur, Amanpreet; Best, Norman B.; Hartwig, Thomas; Budka, Josh; Khangura, Rajdeep S.; McKenzie, Steven; Aragón-Raygoza, Alejandro; Strable, Josh; Schulz, Burkhard; Dilkes, Brian P.

doi:10.1093/plphys/kiae147

Title: A maize semi-dwarf mutant reveals a GRAS transcription factor involved in brassinosteroid signaling

Journal Article · 06 May 2024 · Plant Physiology (Bethesda)

DOI: https://doi.org/10.1093/plphys/kiae147 · OSTI ID:2471865

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Purdue Univ., West Lafayette, IN (United States); Purdue Univ., West Lafayette, IN (United States)
US Dept. of Agriculture (USDA), Columbia, MO (United States). Agricultural Research Service (ARS)
Heinrich-Heine-Universität Düsseldorf (Germany)
Purdue Univ., West Lafayette, IN (United States)
North Carolina State University, Raleigh, NC (United States)
Univ. of Maryland, College Park, MD (United States)

Brassinosteroids (BR) and gibberellins (GA) regulate plant height and leaf angle in maize (Zea mays). Mutants with defects in BR or GA biosynthesis or signaling identify components of these pathways and enhance our knowledge about plant growth and development. In this study, we characterized three recessive mutant alleles of GRAS transcription factor 42 (gras42) in maize, a GRAS transcription factor gene orthologous to the DWARF AND LOW TILLERING (DLT) gene of rice (Oryza sativa). These maize mutants exhibited semi-dwarf stature, shorter and wider leaves, and more upright leaf angle. Transcriptome analysis revealed a role for GRAS42 as a determinant of BR signaling. Analysis of the expression consequences from loss of GRAS42 in the gras42-mu1021149 mutant indicated a weak loss of BR signaling in the mutant, consistent with its previously demonstrated role in BR signaling in rice. Loss of BR signaling was also evident by the enhancement of weak BR biosynthetic mutant alleles in double mutants of nana plant1-1 and gras42-mu1021149. The gras42-mu1021149 mutant had little effect on GA-regulated gene expression, suggesting that GRAS42 is not a regulator of core GA signaling genes in maize. Single-cell expression data identified gras42 expressed among cells in the G2/M phase of the cell cycle consistent with its previously demonstrated role in cell cycle gene expression in Arabidopsis (Arabidopsis thaliana). Cis-acting natural variation controlling GRAS42 transcript accumulation was identified by expression genome-wide association study (eGWAS) in maize. Our results demonstrate a conserved role for GRAS42/SCARECROW-LIKE 28 (SCL28)/DLT in BR signaling, clarify the role of this gene in GA signaling, and suggest mechanisms of tillering and leaf angle control by BR.

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Research Organization:: Donald Danforth Plant Science Center, St. Louis, MO (United States)

Sponsoring Organization:: National Science Foundation (NSF); USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: SC0023305

OSTI ID:: 2471865

Journal Information:: Plant Physiology (Bethesda), Journal Name: Plant Physiology (Bethesda) Journal Issue: 4 Vol. 195; ISSN 0032-0889

Publisher:: American Society of Plant BiologistsCopyright Statement

Country of Publication:: United States

Language:: English

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