Sustained bacterial N2O reduction at acidic pH

He, Guang; Chen, Gao; Xie, Yongchao; Swift, Cynthia M.; Ramirez, Diana; Cha, Gyuhyon; Konstantinidis, Konstantinos T.; Radosevich, Mark; Löffler, Frank E.

doi:10.1038/s41467-024-48236-x

Title: Sustained bacterial N₂O reduction at acidic pH

Journal Article · 14 May 2024 · Nature Communications

DOI: https://doi.org/10.1038/s41467-024-48236-x · OSTI ID:2471421

^[1];

^[1]; Xie, Yongchao ^[2];

^[1]; Ramirez, Diana ^[3]; Cha, Gyuhyon ^[4];

^[4]; Radosevich, Mark ^[1];

^[3]

Univ. of Tennessee, Knoxville, TN (United States)
Univ. of California, Los Angeles, CA (United States); Univ. of Tennessee, Knoxville, TN (United States)
Univ. of Tennessee, Knoxville, TN (United States); Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
Georgia Institute of Technology, Atlanta, GA (United States)

Nitrous oxide (N₂O) is a climate-active gas with emissions predicted to increase due to agricultural intensification. Microbial reduction of N₂O to dinitrogen (N₂) is the major consumption process but microbial N₂O reduction under acidic conditions is considered negligible, albeit strongly acidic soils harbor nosZ genes encoding N₂O reductase. Here, we study a co-culture derived from acidic tropical forest soil that reduces N₂O at pH 4.5. The co-culture exhibits bimodal growth with a Serratia sp. fermenting pyruvate followed by hydrogenotrophic N₂O reduction by a Desulfosporosinus sp. Integrated omics and physiological characterization revealed interspecies nutritional interactions, with the pyruvate fermenting Serratia sp. supplying amino acids as essential growth factors to the N₂O-reducing Desulfosporosinus sp. Thus, we demonstrate growth-linked N₂O reduction between pH 4.5 and 6, highlighting microbial N₂O reduction potential in acidic soils.

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Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: National Science Foundation (NSF); USDOE

Grant/Contract Number:: AC05-00OR22725

OSTI ID:: 2471421

Journal Information:: Nature Communications, Journal Name: Nature Communications Journal Issue: 1 Vol. 15; ISSN 2041-1723

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

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