The contribution of BvgR, RisA, and RisS to global gene regulation, intracellular cyclic-di-GMP levels, motility, and biofilm formation in Bordetella bronchiseptica

Nicholson, Tracy L.; Waack, Ursula; Fleming, Damarius S.; Chen, Qing; Miller, Laura C.; Merkel, Tod J.; Stibitz, Scott

doi:10.3389/fmicb.2024.1305097

Title: The contribution of BvgR, RisA, and RisS to global gene regulation, intracellular cyclic-di-GMP levels, motility, and biofilm formation in Bordetella bronchiseptica

Journal Article · 06 March 2024 · Frontiers in Microbiology

DOI: https://doi.org/10.3389/fmicb.2024.1305097 · OSTI ID:2471303

Nicholson, Tracy L. ^[1]; Waack, Ursula ^[2]; Fleming, Damarius S. ^[3]; Chen, Qing ^[4]; Miller, Laura C. ^[5]; Merkel, Tod J. ^[4]; Stibitz, Scott ^[4]

US Dept. of Agriculture (USDA), Ames, IA (United States). Agricultural Research Service (ARS), National Animal Disease Center
US Dept. of Agriculture (USDA), Ames, IA (United States). Agricultural Research Service (ARS), National Animal Disease Center; Oak Ridge Institute for Science and Education (ORISE), Oak Ridge, TN (United States)
US Dept. of Agriculture (USDA), Beltsville, MD (United States). Beltsville Agricultural Research Service
U.S. Food and Drug Administration (FDA), Silver Spring, MD (United States). Center for Biologics Evaluation and Research
Kansas State Univ., Manhattan, KS (United States)

Bordetella bronchisepticais a highly contagious respiratory bacterial veterinary pathogen. In this study the contribution of the transcriptional regulators BvgR, RisA, RisS, and the phosphorylation of RisA to global gene regulation, intracellular cyclic-di-GMP levels, motility, and biofilm formation were evaluated. Next Generation Sequencing (RNASeq) was used to differentiate the global gene regulation of both virulence-activated and virulence-repressed genes by each of these factors. The BvgAS system, along with BvgR, RisA, and the phosphorylation of RisA served in cyclic-di-GMP degradation. BvgR and unphosphorylated RisA were found to temporally regulate motility. Additionally, BvgR, RisA, and RisS were found to be required for biofilm formation.

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Research Organization:: Oak Ridge Institute for Science and Education (ORISE), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE Office of Science (SC); USDA; USFDA

Grant/Contract Number:: SC0014664

OSTI ID:: 2471303

Journal Information:: Frontiers in Microbiology, Journal Name: Frontiers in Microbiology Vol. 15; ISSN 1664-302X

Publisher:: Frontiers Research FoundationCopyright Statement

Country of Publication:: United States

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
Bordetella bronchiseptica
Microbiology
RNA-seq
biofilm
cyclic-di-GMP
motility

Title: The contribution of BvgR, RisA, and RisS to global gene regulation, intracellular cyclic-di-GMP levels, motility, and biofilm formation in Bordetella bronchiseptica

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References (47)

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