Characterization of Levan Fructan Produced by a Gluconobacter japonicus Strain Isolated from a Sugarcane Processing Facility

Bruni, Gillian O.; Qi, Yunci; Terrell, Evan; Dupre, Rebecca A.; Mattison, Christopher P.

doi:10.3390/microorganisms12010107

Title: Characterization of Levan Fructan Produced by a Gluconobacter japonicus Strain Isolated from a Sugarcane Processing Facility

Journal Article · 04 January 2024 · Microorganisms

DOI: https://doi.org/10.3390/microorganisms12010107 · OSTI ID:2471302

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US Dept. of Agriculture (USDA), New Orleans, LA (United States). Agricultural Research Service (ARS)
US Dept. of Agriculture (USDA), New Orleans, LA (United States). Agricultural Research Service (ARS); Oak Ridge Institute for Science and Education (ORISE), Oak Ridge, TN (United States)

During raw sugarcane processing, a significant portion of lost sucrose is attributable to microbial degradation. Sucrose consumption by many bacteria is also linked to the production of exopolysaccharides (EPS) such as dextrans and fructans. These resulting EPS cause operational challenges during raw sugar manufacturing. Here, we report the characterization of EPS from a fructan-forming Gluconobacter japonicus bacterium that we previously isolated from a Louisiana sugarcane factory. The genome sequencing revealed the presence of two encoded levansucrase genes, lsrA and lsrB. One levansucrase, LsrB, was detected in the secreted protein fraction of G. japonicus LASM12 by QTOF LC-MS. The spotting assays indicated that G. japonicus produces EPS using sucrose and raffinose as substrates. The G. japonicus EPS correlated with levan fructan commercial standards by 1H-NMR, and with the characteristic carbohydrate fingerprint region for FTIR spectra, confirming that the G. japonicus EPS is levan fructan. The glycosyl composition and glycosyl linkage analysis revealed a linear β-2,6-fructofuranosyl polysaccharide with occasional (5.7%) β-2,1-fructofuranosyl branches. The gel permeation chromatography of the levan fructan EPS showed two main peaks at 4.5 kDa and 8 kDa and a very minor peak at 500 kDa. G. japonicus was identified as a producer of levan fructan. These findings will be useful for future studies aimed at evaluating the impact of levan fructans on sugar crop processing, which have been historically underestimated in industry.

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Research Organization:: Oak Ridge Institute for Science and Education (ORISE), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE Office of Science (SC); US Department of Agriculture USDA)

Grant/Contract Number:: SC0014664

OSTI ID:: 2471302

Journal Information:: Microorganisms, Journal Name: Microorganisms Journal Issue: 1 Vol. 12; ISSN 2076-2607; ISSN MICRKN

Publisher:: MDPICopyright Statement

Country of Publication:: United States

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
Microbiology
exopolysaccharide
levan fructan
levansucrase
sugar processing
sugarcane

Title: Characterization of Levan Fructan Produced by a Gluconobacter japonicus Strain Isolated from a Sugarcane Processing Facility

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