Separation of Lipoproteins for Quantitative Analysis of 14C-Labeled Lipid-Soluble Compounds by Accelerator Mass Spectrometry

Chuang, Jennifer C.; Clifford, Andrew J.; Kim, Seung-Hyun; Novotny, Janet A.; Kelly, Peter B.; Holstege, Dirk M.; Walzem, Rosemary L.

doi:10.3390/ijms25031856

Title: Separation of Lipoproteins for Quantitative Analysis of ¹⁴C-Labeled Lipid-Soluble Compounds by Accelerator Mass Spectrometry

Journal Article · 02 February 2024 · International Journal of Molecular Sciences (Online)

DOI: https://doi.org/10.3390/ijms25031856 · OSTI ID:2471159

^[1]; Clifford, Andrew J. ^[2]; Kim, Seung-Hyun ^[3];

^[4]; Kelly, Peter B. ^[2]; Holstege, Dirk M. ^[2];

^[5]

Nutrilite, Buena Park, CA (United States)
University of California, Davis, CA (United States)
Konkuk University, Seoul (Korea, Republic of)
U.S. Department of Agriculture, Beltsville, MD (United States)
Texas A&M University, College Station, TX (United States)

To date, ¹⁴C tracer studies using accelerator mass spectrometry (AMS) have not yet resolved lipid-soluble analytes into individual lipoprotein density subclasses. The objective of this work was to develop a reliable method for lipoprotein separation and quantitative recovery for biokinetic modeling purposes. The novel method developed provides the means for use of small volumes (10–200 µL) of frozen plasma as a starting material for continuous isopycnic lipoprotein separation within a carbon- and pH-stable analyte matrix, which, following post-separation fraction clean up, created samples suitable for highly accurate ¹⁴C/¹²C isotope ratio determinations by AMS. Manual aspiration achieved 99.2 ± 0.41% recovery of [5-¹⁴CH₃]-(2R, 4'R, 8'R)-α-tocopherol contained within 25 µL plasma recovered in triacylglycerol rich lipoproteins (TRL = Chylomicrons + VLDL), LDL, HDL, and infranatant (INF) from each of 10 different sampling times for one male and one female subject, n = 20 total samples. Small sample volumes of previously frozen plasma and high analyte recoveries make this an attractive method for AMS studies using newer, smaller footprint AMS equipment to develop genuine tracer analyses of lipophilic nutrients or compounds in all human age ranges.

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Research Organization:: Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)

Sponsoring Organization:: USDOE; National Institutes of Health (NIH)

Grant/Contract Number:: AC52-07NA27344

OSTI ID:: 2471159

Journal Information:: International Journal of Molecular Sciences (Online), Journal Name: International Journal of Molecular Sciences (Online) Journal Issue: 3 Vol. 25; ISSN 1422-0067

Publisher:: MDPICopyright Statement

Country of Publication:: United States

Language:: English

References (27)

Pharmacokinetic analysis of ¹⁴C‐ursodiol in newborn infants using accelerator mass spectrometry Gordi, Toufigh; Baillie, Rebecca; Vuong, Le T. The Journal of Clinical Pharmacology, Vol. 54, Issue 9 https://doi.org/10.1002/jcph.327	journal	May 2014
Studies on the transfer of tocopherol between lipoproteins Traber, Maret G.; Lane, Jerome C.; Lagmay, Nora R. Lipids, Vol. 27, Issue 9 https://doi.org/10.1007/BF02536020	journal	September 1992
Isolation of remnant particles by immunoseparation: a new approach for investigation of postprandial lipoprotein metabolism in normolipidemic subjects Schreuder, P. C. N. J.; Twickler, Th. B.; Wang, T. Atherosclerosis, Vol. 157, Issue 1 https://doi.org/10.1016/S0021-9150(00)00666-3	journal	July 2001
Changes in plasma very low density and low density lipoprotein content, composition, and size after a fatty meal in normo- and hypertriglyceridemic man. Redgrave, T. G.; Carlson, L. A. Journal of Lipid Research, Vol. 20, Issue 2 https://doi.org/10.1016/S0022-2275(20)40633-9	journal	February 1979
Quantitating Isotopic Molecular Labels with Accelerator Mass Spectrometry Vogel, John S.; Love, Adam H. Methods in Enzymology https://doi.org/10.1016/S0076-6879(05)02013-6	book	January 2005
Double Trap Interface: A novel gas interface for high throughput analysis of biomedical samples by AMS De Maria, Daniele; Fahrni, Simon M.; Lozac'h, Frédéric Drug Metabolism and Pharmacokinetics, Vol. 39 https://doi.org/10.1016/j.dmpk.2021.100400	journal	August 2021
UC/MALDI-MS analysis of HDL; evidence for density-dependent post-translational modifications Johnson, Jeffery D.; Henriquez, Ronald R.; Tichy, Shane E. International Journal of Mass Spectrometry, Vol. 268, Issue 2-3 https://doi.org/10.1016/j.ijms.2007.08.010	journal	December 2007
MICADAS: A new compact radiocarbon AMS system Synal, Hans-Arno; Stocker, Martin; Suter, Martin Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms, Vol. 259, Issue 1 https://doi.org/10.1016/j.nimb.2007.01.138	journal	June 2007
Metal Ion Complexes of EDTA: A Solute System for Density Gradient Ultracentrifugation Analysis of Lipoproteins Hosken, Brian D.; Cockrill, Steven L.; Macfarlane, Ronald D. Analytical Chemistry, Vol. 77, Issue 1 https://doi.org/10.1021/ac0490402	journal	December 2004
Remnant Lipoprotein Density Profiling by CsBiEDTA Density Gradient Ultracentrifugation Chandra, Richa; Macfarlane, Ronald D. Analytical Chemistry, Vol. 78, Issue 3 https://doi.org/10.1021/ac050775w	journal	December 2005
Method for Lipoprotein(a) Density Profiling by BiEDTA Differential Density Lipoprotein Ultracentrifugation Espinosa, I. Leticia; McNeal, Catherine J.; Macfarlane, Ronald D. Analytical Chemistry, Vol. 78, Issue 2 https://doi.org/10.1021/ac050962u	journal	December 2005
Metal Ion Complexes of EDTA as Solutes for Density Gradient Ultracentrifugation: Influence of Metal Ions Johnson, Jeffery D.; Bell, Natalie J.; Donahoe, Erin L. Analytical Chemistry, Vol. 77, Issue 21 https://doi.org/10.1021/ac0509657	journal	September 2005
Carbon Isotopes Profiles of Human Whole Blood, Plasma, Red Blood Cells, Urine and Feces for Biological/Biomedical ¹⁴C-Accelerator Mass Spectrometry Applications Kim, Seung-Hyun; Chuang, Jennifer C.; Kelly, Peter B. Analytical Chemistry, Vol. 83, Issue 9 https://doi.org/10.1021/ac103038s	journal	March 2011
A radiocarbon revolution sheds light on the Vikings Barrett, James H. Nature, Vol. 601, Issue 7893 https://doi.org/10.1038/d41586-021-03769-9	journal	December 2021
Biological and biomedical 14C-accelerator mass spectrometry and graphitization of carbonaceous samples Chung, Ill-Min; Kim, Seung-Hyun The Analyst, Vol. 138, Issue 12 https://doi.org/10.1039/c3an00077j	journal	January 2013
A feasibility study quantifying in vivo human α-tocopherol metabolism Clifford, Andrew J.; de Moura, Fabiana F.; Ho, Charlene C. The American Journal of Clinical Nutrition, Vol. 84, Issue 6 https://doi.org/10.1093/ajcn/84.6.1430	journal	December 2006
The Distribution and Chemical Composition of Ultracentrifugally Separated Lipoproteins in Human Serum Havel, Richard J.; Eder, Howard A.; Bragdon, Joseph H. Journal of Clinical Investigation, Vol. 34, Issue 9 https://doi.org/10.1172/JCI103182	journal	September 1955
Complexity of microRNA function and the role of isomiRs in lipid homeostasis Vickers, Kasey C.; Sethupathy, Praveen; Baran-Gale, Jeanette Journal of Lipid Research, Vol. 54, Issue 5 https://doi.org/10.1194/jlr.R034801	journal	May 2013
HDL and cholesterol: life after the divorce? Vickers, Kasey C.; Remaley, Alan T. Journal of Lipid Research, Vol. 55, Issue 1 https://doi.org/10.1194/jlr.R035964	journal	January 2014
Chylomicrons produced by Caco-2 cells contained ApoB-48 with diameter of 80-200 nm Nauli, Andromeda M.; Sun, Yuxi; Whittimore, Judy D. Physiological Reports, Vol. 2, Issue 6 https://doi.org/10.14814/phy2.12018	journal	June 2014
14C-Cobalamin Absorption from Endogenously Labeled Chicken Eggs Assessed in Humans Using Accelerator Mass Spectrometry Nutrients, Vol. 11, Issue 9 https://doi.org/10.3390/nu11092148	journal	September 2019
Baseline Insulin Resistance Is a Determinant of the Small, Dense Low-Density Lipoprotein Response to Diets Differing in Saturated Fat, Protein, and Carbohydrate Contents Wu, Xiuzhi; Roussell, Michael A.; Hill, Alison M. Nutrients, Vol. 13, Issue 12 https://doi.org/10.3390/nu13124328	journal	November 2021
Radiocarbon Tracers in Toxicology and Medicine: Recent Advances in Technology and Science Malfatti, Michael A.; Buchholz, Bruce A.; Enright, Heather A. Toxics, Vol. 7, Issue 2 https://doi.org/10.3390/toxics7020027	journal	May 2019
Lycopene bioavailability and metabolism in humans: an accelerator mass spectrometry study Ross, Alastair B.; Vuong, Le Thuy; Ruckle, Jon The American Journal of Clinical Nutrition, Vol. 93, Issue 6 https://doi.org/10.3945/ajcn.110.008375	journal	June 2011
Accelerator Mass Spectrometry Can Be Used to Assess Vitamin A Metabolism Quantitatively in Boys in a Community Setting Aklamati, Emmanuel K.; Mulenga, Modest; Dueker, Stephen R. The Journal of Nutrition, Vol. 140, Issue 9 https://doi.org/10.3945/jn.110.125500	journal	September 2010
Quantitation of [5-CH3]-(2R, 4'R, 8'R)-α-Tocopherol in Humans Chuang, Jennifer C.; Matel, Hosea D.; Nambiar, Krishnan P. The Journal of Nutrition, Vol. 141, Issue 8 https://doi.org/10.3945/jn.111.138925	journal	August 2011
This Kinetic, Bioavailability, and Metabolism Study of RRR-α-Tocopherol in Healthy Adults Suggests Lower Intake Requirements than Previous Estimates, Novotny, Janet A.; Fadel, James G.; Holstege, Dirk M. The Journal of Nutrition, Vol. 142, Issue 12 https://doi.org/10.3945/jn.112.166462	journal	December 2012

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37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
59 BASIC BIOLOGICAL SCIENCES
accelerator mass spectrometry
biochemistry & molecular biology
chemistry
fluorescent imaging
isopycnic centrifugation
lipoproteins
α-tocopherol tracer analysis

Title: Separation of Lipoproteins for Quantitative Analysis of 14C-Labeled Lipid-Soluble Compounds by Accelerator Mass Spectrometry

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References (27)

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Title: Separation of Lipoproteins for Quantitative Analysis of ¹⁴C-Labeled Lipid-Soluble Compounds by Accelerator Mass Spectrometry