Metabolic model predictions enable targeted microbiome manipulation through precision prebiotics

Marinos, Georgios; Hamerich, Inga K.; Debray, Reena; Obeng, Nancy; Petersen, Carola; Taubenheim, Jan; Zimmermann, Johannes; Blackburn, Dana; Samuel, Buck S.; Dierking, Katja; Franke, Andre; Laudes, Matthias; Waschina, Silvio; Schulenburg, Hinrich; Kaleta, Christoph

doi:10.1128/spectrum.01144-23

Title: Metabolic model predictions enable targeted microbiome manipulation through precision prebiotics

Journal Article · 17 January 2024 · Microbiology Spectrum

DOI: https://doi.org/10.1128/spectrum.01144-23 · OSTI ID:2471103

^[1];

^[2];

^[1];

^[3];

^[4];

^[1];

^[3];

^[1]

Univ. of Kiel (Germany)
University of California, Berkeley, CA (United States)
Univ. of Kiel (Germany); Max Planck Institute for Evolutionary Biology, Schleswig-Holstein (Germany)
Baylor College of Medicine, Houston, TX (United States)

While numerous health-beneficial interactions between host and microbiota have been identified, there is still a lack of targeted approaches for modulating these interactions. Thus, we here identify precision prebiotics that specifically modulate the abundance of a microbiome member species of interest. In the first step, we show that defining precision prebiotics by compounds that are only taken up by the target species but no other species in a community is usually not possible due to overlapping metabolic niches. Subsequently, we use metabolic modeling to identify precision prebiotics for a two-member Caenorhabditis elegans microbiome community comprising the immune-protective target species Pseudomonas lurida MYb11 and the persistent colonizer Ochrobactrum vermis MYb71. We experimentally confirm four of the predicted precision prebiotics, L-serine, L-threonine, D-mannitol, and γ-aminobutyric acid, to specifically increase the abundance of MYb11. L-serine was further assessed in vivo, leading to an increase in MYb11 abundance also in the worm host. Overall, our findings demonstrate that metabolic modeling is an effective tool for the design of precision prebiotics as an important cornerstone for future microbiome-targeted therapies.

View Accepted Manuscript (DOE)

Cite

Export

Save

Research Organization:: USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States)

Sponsoring Organization:: USDOE; National Institutes of Health (NIH); National Aeronautics and Space Administration (NASA)

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 2471103

Journal Information:: Microbiology Spectrum, Journal Name: Microbiology Spectrum Journal Issue: 2 Vol. 12; ISSN 2165-0497

Publisher:: American Society for MicrobiologyCopyright Statement

Country of Publication:: United States

Language:: English

References (79)

ggplot2 Wickham, Hadley Use R! https://doi.org/10.1007/978-3-319-24277-4	book	January 2016
Gut microbiota functions: metabolism of nutrients and other food components Rowland, Ian; Gibson, Glenn; Heinken, Almut European Journal of Nutrition, Vol. 57, Issue 1 https://doi.org/10.1007/s00394-017-1445-8	journal	April 2017
Advances in colon-targeted nano-drug delivery systems: challenges and solutions Naeem, Muhammad; Awan, Uzma Azeem; Subhan, Fazli Archives of Pharmacal Research, Vol. 43, Issue 1 https://doi.org/10.1007/s12272-020-01219-0	journal	January 2020
Advanced drug delivery 2020 and beyond: Perspectives on the future Bae, You Han; Park, Kinam Advanced Drug Delivery Reviews, Vol. 158 https://doi.org/10.1016/j.addr.2020.06.018	journal	January 2020
Prevalence of virulence genes and antibiotic susceptibility of Bacillus used in commercial aquaculture probiotics in China Anokyewaa, Melody Abena; Amoah, Kwaku; Li, Yuan Aquaculture Reports, Vol. 21 https://doi.org/10.1016/j.aqrep.2021.100784	journal	November 2021
Host-Microbe-Drug-Nutrient Screen Identifies Bacterial Effectors of Metformin Therapy Pryor, Rosina; Norvaisas, Povilas; Marinos, Georgios Cell, Vol. 178, Issue 6 https://doi.org/10.1016/j.cell.2019.08.003	journal	September 2019
Stable Engraftment of Bifidobacterium longum AH1206 in the Human Gut Depends on Individualized Features of the Resident Microbiome Maldonado-Gómez, María X.; Martínez, Inés; Bottacini, Francesca Cell Host & Microbe, Vol. 20, Issue 4 https://doi.org/10.1016/j.chom.2016.09.001	journal	October 2016
Impairing L-Threonine Catabolism Promotes Healthspan through Methylglyoxal-Mediated Proteohormesis Ravichandran, Meenakshi; Priebe, Steffen; Grigolon, Giovanna Cell Metabolism, Vol. 27, Issue 4 https://doi.org/10.1016/j.cmet.2018.02.004	journal	April 2018
Natural C. elegans Microbiota Protects against Infection via Production of a Cyclic Lipopeptide of the Viscosin Group Kissoyan, Kohar A. B.; Drechsler, Moritz; Stange, Eva-Lena Current Biology, Vol. 29, Issue 6 https://doi.org/10.1016/j.cub.2019.01.050	journal	March 2019
Natural genetic variation drives microbiome selection in the Caenorhabditis elegans gut Zhang, Fan; Weckhorst, Jessica L.; Assié, Adrien Current Biology, Vol. 31, Issue 12 https://doi.org/10.1016/j.cub.2021.04.046	journal	June 2021
Regulation, risk and safety of Faecal Microbiota Transplant Merrick, Blair; Allen, Liz; Masirah M. Zain, Nur Infection Prevention in Practice, Vol. 2, Issue 3 https://doi.org/10.1016/j.infpip.2020.100069	journal	September 2020
Microbiome-Mediated Stress Resistance in Plants Liu, Hongwei; Brettell, Laura E.; Qiu, Zhiguang Trends in Plant Science, Vol. 25, Issue 8 https://doi.org/10.1016/j.tplants.2020.03.014	journal	August 2020
Natural variation in the response of Caenorhabditis elegans towards Bacillus thuringiensis Schulenburg, H.; Mller, S. Parasitology, Vol. 128, Issue 4 https://doi.org/10.1017/s003118200300461x	journal	April 2004
A Primer on Effectiveness and Efficacy Trials Singal, Amit G.; Higgins, Peter D. R.; Waljee, Akbar K. Clinical and Translational Gastroenterology, Vol. 5, Issue 1 https://doi.org/10.1038/ctg.2013.13	journal	January 2014
What is flux balance analysis? Orth, Jeffrey D.; Thiele, Ines; Palsson, Bernhard Ø Nature Biotechnology, Vol. 28, Issue 3 https://doi.org/10.1038/nbt.1614	journal	March 2010
Generation of genome-scale metabolic reconstructions for 773 members of the human gut microbiota Magnúsdóttir, Stefanía; Heinken, Almut; Kutt, Laura Nature Biotechnology, Vol. 35, Issue 1 https://doi.org/10.1038/nbt.3703	journal	November 2016
Control of pathogens and pathobionts by the gut microbiota Kamada, Nobuhiko; Chen, Grace Y.; Inohara, Naohiro Nature Immunology, Vol. 14, Issue 7 https://doi.org/10.1038/ni.2608	journal	June 2013
Constraint-based models predict metabolic and associated cellular functions Bordbar, Aarash; Monk, Jonathan M.; King, Zachary A. Nature Reviews Genetics, Vol. 15, Issue 2 https://doi.org/10.1038/nrg3643	journal	January 2014
Microbiota in health and diseases Hou, Kaijian; Wu, Zhuo-Xun; Chen, Xuan-Yu Signal Transduction and Targeted Therapy, Vol. 7, Issue 1 https://doi.org/10.1038/s41392-022-00974-4	journal	April 2022
The functional repertoire contained within the native microbiota of the model nematode Caenorhabditis elegans Zimmermann, Johannes; Obeng, Nancy; Yang, Wentao The ISME Journal, Vol. 14, Issue 1 https://doi.org/10.1038/s41396-019-0504-y	journal	September 2019
Interaction between microbiota and immunity in health and disease Zheng, Danping; Liwinski, Timur; Elinav, Eran Cell Research, Vol. 30, Issue 6 https://doi.org/10.1038/s41422-020-0332-7	journal	May 2020
Dietary serine-microbiota interaction enhances chemotherapeutic toxicity without altering drug conversion Ke, Wenfan; Saba, James A.; Yao, Cong-Hui Nature Communications, Vol. 11, Issue 1 https://doi.org/10.1038/s41467-020-16220-w	journal	May 2020
The microbiome extends host evolutionary potential Henry, Lucas P.; Bruijning, Marjolein; Forsberg, Simon K. G. Nature Communications, Vol. 12, Issue 1 https://doi.org/10.1038/s41467-021-25315-x	journal	August 2021
L-threonine promotes healthspan by expediting ferritin-dependent ferroptosis inhibition in C. elegans Kim, Juewon; Jo, Yunju; Cho, Donghyun Nature Communications, Vol. 13, Issue 1 https://doi.org/10.1038/s41467-022-34265-x	journal	November 2022
The evolution of commercial drug delivery technologies Vargason, Ava M.; Anselmo, Aaron C.; Mitragotri, Samir Nature Biomedical Engineering, Vol. 5, Issue 9 https://doi.org/10.1038/s41551-021-00698-w	journal	April 2021
Dietary l-serine confers a competitive fitness advantage to Enterobacteriaceae in the inflamed gut Kitamoto, Sho; Alteri, Christopher J.; Rodrigues, Michael Nature Microbiology, Vol. 5, Issue 1 https://doi.org/10.1038/s41564-019-0591-6	journal	November 2019
Microbiome-based therapeutics Sorbara, Matthew T.; Pamer, Eric G. Nature Reviews Microbiology, Vol. 20, Issue 6 https://doi.org/10.1038/s41579-021-00667-9	journal	January 2022
Metabolic Functions of Gut Microbes Associate With Efficacy of Tumor Necrosis Factor Antagonists in Patients With Inflammatory Bowel Diseases Aden, Konrad; Rehman, Ateequr; Waschina, Silvio Gastroenterology, Vol. 157, Issue 5 https://doi.org/10.1053/j.gastro.2019.07.025	journal	November 2019
The gut microbiome: Relationships with disease and opportunities for therapy Durack, Juliana; Lynch, Susan V. Journal of Experimental Medicine, Vol. 216, Issue 1 https://doi.org/10.1084/jem.20180448	journal	October 2018
Microbiome Modelling Toolbox 2.0: efficient, tractable modelling of microbiome communities Heinken, Almut; Thiele, Ines Bioinformatics, Vol. 38, Issue 8 https://doi.org/10.1093/bioinformatics/btac082	journal	February 2022
Microbial Butyrate Synthesis Indicates Therapeutic Efficacy of Azathioprine in IBD Patients Effenberger, M.; Reider, S.; Waschina, S. Journal of Crohn's and Colitis, Vol. 15, Issue 1 https://doi.org/10.1093/ecco-jcc/jjaa152	journal	December 2020
HMDB 5.0: the Human Metabolome Database for 2022 Wishart, David S.; Guo, AnChi; Oler, Eponine Nucleic Acids Research, Vol. 50, Issue D1 https://doi.org/10.1093/nar/gkab1062	journal	November 2021
Fast automated reconstruction of genome-scale metabolic models for microbial species and communities Machado, Daniel; Andrejev, Sergej; Tramontano, Melanie Nucleic Acids Research, Vol. 46, Issue 15 https://doi.org/10.1093/nar/gky537	journal	June 2018
The role ofCaenorhabditis elegansinsulin‐like signaling in the behavioral avoidance of pathogenicBacillus thuringiensis Hasshoff, Martin; Höhnisch, Claudia; Tonn, Daniela The FASEB Journal, Vol. 21, Issue 8 https://doi.org/10.1096/fj.06-6551com	journal	February 2007
Constraint-based stoichiometric modelling from single organisms to microbial communities Gottstein, Willi; Olivier, Brett G.; Bruggeman, Frank J. Journal of The Royal Society Interface, Vol. 13, Issue 124 https://doi.org/10.1098/rsif.2016.0627	journal	November 2016
Microbiome-mediated plasticity directs host evolution along several distinct time scales Kolodny, Oren; Schulenburg, Hinrich Philosophical Transactions of the Royal Society B: Biological Sciences, Vol. 375, Issue 1808 https://doi.org/10.1098/rstb.2019.0589	journal	August 2020
The Aerobic Pseudomonads a Taxonomic Study Stanier, R. Y.; Palleroni, N. J.; Doudoroff, M. Journal of General Microbiology, Vol. 43, Issue 2 https://doi.org/10.1099/00221287-43-2-159	journal	May 1966
Community assembly of the native C . elegans microbiome is influenced by time, substrate and individual bacterial taxa Johnke, Julia; Dirksen, Philipp; Schulenburg, Hinrich Environmental Microbiology, Vol. 22, Issue 4 https://doi.org/10.1111/1462-2920.14932	journal	April 2020
Gut Reactions: Breaking Down Xenobiotic–Microbiome Interactions Clarke, Gerard; Sandhu, Kiran V.; Griffin, Brendan T. Pharmacological Reviews, Vol. 71, Issue 2 https://doi.org/10.1124/pr.118.015768	journal	February 2019
Modernized Tools for Streamlined Genetic Manipulation and Comparative Study of Wild and Diverse Proteobacterial Lineages Wiles, Travis J.; Wall, Elena S.; Schlomann, Brandon H. mBio, Vol. 9, Issue 5 https://doi.org/10.1128/mBio.01877-18	journal	October 2018
Interaction between drugs and the gut microbiome Weersma, Rinse K.; Zhernakova, Alexandra; Fu, Jingyuan Gut, Vol. 69, Issue 8 https://doi.org/10.1136/gutjnl-2019-320204	journal	May 2020
Host-Microbe Interactions inCaenorhabditis elegans Zhang, Rui; Hou, Aixin ISRN Microbiology, Vol. 2013 https://doi.org/10.1155/2013/356451	journal	August 2013
PopGen: Population-Based Recruitment of Patients and Controls for the Analysis of Complex Genotype-Phenotype Relationships Krawczak, Michael; Nikolaus, Susanna; von Eberstein, Huberta Public Health Genomics, Vol. 9, Issue 1 https://doi.org/10.1159/000090694	journal	January 2006
sybil – Efficient constraint-based modelling in R Gelius-Dietrich, Gabriel; Desouki, Abdelmoneim; Fritzemeier, Claus BMC Systems Biology, Vol. 7, Issue 1 https://doi.org/10.1186/1752-0509-7-125	journal	January 2013
The nutritional requirements of Caenorhabditis elegans Zečić, Aleksandra; Dhondt, Ineke; Braeckman, Bart P. Genes & Nutrition, Vol. 14, Issue 1 https://doi.org/10.1186/s12263-019-0637-7	journal	May 2019
Contrasting invertebrate immune defense behaviors caused by a single gene, the Caenorhabditis elegans neuropeptide receptor gene npr-1 Nakad, Rania; Snoek, L. Basten; Yang, Wentao BMC Genomics, Vol. 17, Issue 1 https://doi.org/10.1186/s12864-016-2603-8	journal	April 2016
The native microbiome of the nematode Caenorhabditis elegans: gateway to a new host-microbiome model Dirksen, Philipp; Marsh, Sarah Arnaud; Braker, Ines BMC Biology, Vol. 14, Issue 1 https://doi.org/10.1186/s12915-016-0258-1	journal	May 2016
Parkinson’s disease-associated alterations of the gut microbiome predict disease-relevant changes in metabolic functions Baldini, Federico; Hertel, Johannes; Sandt, Estelle BMC Biology, Vol. 18, Issue 1 https://doi.org/10.1186/s12915-020-00775-7	journal	June 2020
gapseq: informed prediction of bacterial metabolic pathways and reconstruction of accurate metabolic models Zimmermann, Johannes; Kaleta, Christoph; Waschina, Silvio Genome Biology, Vol. 22, Issue 1 https://doi.org/10.1186/s13059-021-02295-1	journal	March 2021
Nanoparticles: Oral Delivery for Protein and Peptide Drugs Cao, Shu-jun; Xu, Shuo; Wang, Hui-ming AAPS PharmSciTech, Vol. 20, Issue 5 https://doi.org/10.1208/s12249-019-1325-z	journal	May 2019
Recent advances in modulating the microbiome Quigley, Eamonn M. M.; Gajula, Prianka F1000Research, Vol. 9 https://doi.org/10.12688/f1000research.20204.1	journal	January 2020
Bacterially produced metabolites protect C. elegans neurons from degeneration Urrutia, Arles; García-Angulo, Víctor A.; Fuentes, Andrés PLOS Biology, Vol. 18, Issue 3 https://doi.org/10.1371/journal.pbio.3000638	journal	March 2020
BacArena: Individual-based metabolic modeling of heterogeneous microbes in complex communities Bauer, Eugen; Zimmermann, Johannes; Baldini, Federico PLOS Computational Biology, Vol. 13, Issue 5 https://doi.org/10.1371/journal.pcbi.1005544	journal	May 2017
Glycine promotes longevity in Caenorhabditis elegans in a methionine cycle-dependent fashion Liu, Yasmine J.; Janssens, Georges E.; McIntyre, Rebecca L. PLOS Genetics, Vol. 15, Issue 3 https://doi.org/10.1371/journal.pgen.1007633	journal	March 2019
Defining the nutritional input for genome-scale metabolic models: A roadmap Marinos, Georgios; Kaleta, Christoph; Waschina, Silvio PLOS ONE, Vol. 15, Issue 8 https://doi.org/10.1371/journal.pone.0236890	journal	August 2020
d-Serine and d-Alanine Regulate Adaptive Foraging Behavior in Caenorhabditis elegans via the NMDA Receptor Saitoh, Yasuaki; Katane, Masumi; Miyamoto, Tetsuya The Journal of Neuroscience, Vol. 40, Issue 39 https://doi.org/10.1523/JNEUROSCI.2358-19.2020	journal	August 2020
Enhancing nutritional niche and host defenses by modifying the gut microbiome Sun, Qing; Vega, Nic M.; Cervantes, Bernardo Molecular Systems Biology, Vol. 18, Issue 11 https://doi.org/10.15252/msb.20209933	journal	November 2022
CeMbio - The Caenorhabditis elegans Microbiome Resource Dirksen, Philipp; Assié, Adrien; Zimmermann, Johannes G3 Genes\|Genomes\|Genetics, Vol. 10, Issue 9 https://doi.org/10.1534/g3.120.401309	journal	September 2020
Maintenance of C. elegans Stiernagle, Theresa WormBook https://doi.org/10.1895/wormbook.1.101.1	journal	January 2006
Welcome to the Tidyverse Wickham, Hadley; Averick, Mara; Bryan, Jennifer Journal of Open Source Software, Vol. 4, Issue 43 https://doi.org/10.21105/joss.01686	journal	November 2019
Individual Comparisons by Ranking Methods Wilcoxon, Frank Biometrics Bulletin, Vol. 1, Issue 6 https://doi.org/10.2307/3001968	journal	December 1945
Targeted Microbiome Intervention by Microencapsulated Delayed-Release Niacin Beneficially Affects Insulin Sensitivity in Humans Fangmann, Daniela; Theismann, Eva-Maria; Türk, Kathrin Diabetes Care, Vol. 41, Issue 3 https://doi.org/10.2337/dc17-1967	journal	December 2017
Exploring Effects of C. elegans Protective Natural Microbiota on Host Physiology Kissoyan, Kohar Annie B.; Peters, Lena; Giez, Christoph Frontiers in Cellular and Infection Microbiology, Vol. 12 https://doi.org/10.3389/fcimb.2022.775728	journal	February 2022
Effects of Psychological, Environmental and Physical Stressors on the Gut Microbiota Karl, J. Philip; Hatch, Adrienne M.; Arcidiacono, Steven M. Frontiers in Microbiology, Vol. 9 https://doi.org/10.3389/fmicb.2018.02013	journal	September 2018
A Comparative Review on Microbiota Manipulation: Lessons From Fish, Plants, Livestock, and Human Research Brugman, Sylvia; Ikeda-Ohtsubo, Wakako; Braber, Saskia Frontiers in Nutrition, Vol. 5 https://doi.org/10.3389/fnut.2018.00080	journal	September 2018
Constraint-Based Reconstruction and Analyses of Metabolic Models: Open-Source Python Tools and Applications to Cancer Ng, Rachel H.; Lee, Jihoon W.; Baloni, Priyanka Frontiers in Oncology, Vol. 12 https://doi.org/10.3389/fonc.2022.914594	journal	July 2022
Prebiotics: Definition, Types, Sources, Mechanisms, and Clinical Applications Davani-Davari, Dorna; Negahdaripour, Manica; Karimzadeh, Iman Foods, Vol. 8, Issue 3 https://doi.org/10.3390/foods8030092	journal	March 2019
Targeted Approaches for In Situ Gut Microbiome Manipulation Lee, Hui Ling; Shen, Haosheng; Hwang, In Young Genes, Vol. 9, Issue 7 https://doi.org/10.3390/genes9070351	journal	July 2018
Dietary Supplements: Regulatory Challenges and Research Resources Dwyer, Johanna; Coates, Paul; Smith, Michael Nutrients, Vol. 10, Issue 1 https://doi.org/10.3390/nu10010041	journal	January 2018
Mechanisms of Action of Prebiotics and Their Effects on Gastro-Intestinal Disorders in Adults Guarino, Michele; Altomare, Annamaria; Emerenziani, Sara Nutrients, Vol. 12, Issue 4 https://doi.org/10.3390/nu12041037	journal	April 2020
The Impact of Diet on Microbiota Evolution and Human Health. Is Diet an Adequate Tool for Microbiota Modulation? Moles, Laura; Otaegui, David Nutrients, Vol. 12, Issue 6 https://doi.org/10.3390/nu12061654	journal	June 2020
Systems-level characterization of a host-microbe metabolic symbiosis in the mammalian gut Heinken, Almut; Sahoo, Swagatika; Fleming, Ronan M. T. Gut Microbes, Vol. 4, Issue 1 https://doi.org/10.4161/gmic.22370	journal	January 2013
Colon Targeted Drug Delivery Systems: A Review on Primary and Novel Approaches Philip, Anil; Philip, Betty Oman Medical Journal, Vol. 25, Issue 2 https://doi.org/10.5001/omj.2010.24	journal	April 2010
Fecal Microbiota Transplantation: Is It Safe? Park, Seon-Young; Seo, Geom Seog Clinical Endoscopy, Vol. 54, Issue 2 https://doi.org/10.5946/ce.2021.072	journal	March 2021
Metabolic models of human gut and C. eleagans bacterial communities. Marinos, Georgios; Hamerich, Inga K.; Debray, Reena figshare https://doi.org/10.6084/m9.figshare.24968871.v1	other	January 2024
The native microbiome of the nematode Caenorhabditis elegans: gateway to a new host-microbiome model Dirksen, Philipp; Marsh, Sarah; Braker, Ines Figshare https://doi.org/10.6084/m9.figshare.c.3627926	collection	January 2016
Modernized tools for streamlined genetic manipulation and comparative study of wild and diverse proteobacterial lineages Wiles, Travis J.; Wall, Elena S.; Schlomann, Brandon H. Figshare https://doi.org/10.6084/m9.figshare.c.4141163.v1	collection	January 2018
Parkinson’s disease-associated alterations of the gut microbiome predict disease-relevant changes in metabolic functions Baldini, Federico; Hertel, Johannes; Sandt, Estelle figshare https://doi.org/10.6084/m9.figshare.c.5014949.v1	collection	January 2020
gapseq: informed prediction of bacterial metabolic pathways and reconstruction of accurate metabolic models Zimmermann, Johannes; Kaleta, Christoph; Waschina, Silvio figshare https://doi.org/10.6084/m9.figshare.c.5334681	collection	January 2021

Similar Records

CeMbio - The Caenorhabditis elegans Microbiome Resource

Journal Article · 2020 · G3 · OSTI ID:1816499

Dirksen, Philipp; Assié, Adrien; Zimmermann, Johannes; +8 more

Identification and characterization of a skin microbiome on Caenorhabditis elegans suggests environmental microbes confer cuticle protection

Journal Article · 2024 · Microbiology Spectrum · OSTI ID:2396398

Haghani, Nadia B.; Lampe, Robert H.; Samuel, Buck S.; +3 more

Identification of carbohydrate gene clusters obtained from in vitro fermentations as predictive biomarkers of prebiotic responses

Journal Article · 2024 · BMC Microbiology · OSTI ID:2469748

Kok, Car Reen; Rose, Devin J.; Cui, Juan; +2 more

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
Caenorhabditis elegans
Ochrobactrum vermis MYb71
Pseudomonas lurida MYb11
flux balance analysis
genome-scale metabolic models
nutritional supplements
serine

Title: Metabolic model predictions enable targeted microbiome manipulation through precision prebiotics

Citation Formats

References (79)

Linked Research (1)

Similar Records

Related Subjects