Long-term, non-invasive FTIR detection of low-dose ionizing radiation exposure

Inman, Jamie L.; Wu, Yulun; Chen, Liang; Brydon, Ella; Ghosh, Dhruba; Wan, Kenneth H.; De Chant, Jared; Obst-Huebl, Lieselotte; Nakamura, Kei; Ralston, Corie Y.; Celniker, Susan E.; Mao, Jian-Hua; Zwart, Peter H.; Holman, Hoi-Ying N.; Chang, Hang; Brown, James B.; Snijders, Antoine M.

doi:10.1038/s41598-024-56491-7

Title: Long-term, non-invasive FTIR detection of low-dose ionizing radiation exposure

Journal Article · 13 March 2024 · Scientific Reports

DOI: https://doi.org/10.1038/s41598-024-56491-7 · OSTI ID:2470989

Inman, Jamie L. ^[1]; Wu, Yulun ^[2]; Chen, Liang ^[1]; Brydon, Ella ^[1]; Ghosh, Dhruba ^[3]; Wan, Kenneth H. ^[1]; De Chant, Jared ^[1]; Obst-Huebl, Lieselotte ^[1]; Nakamura, Kei ^[1]; Ralston, Corie Y. ^[1]; Celniker, Susan E. ^[1]; Mao, Jian-Hua ^[1]; Zwart, Peter H. ^[1]; Holman, Hoi-Ying N. ^[1]; Chang, Hang ^[1]; Brown, James B. ^[2]; Snijders, Antoine M. ^[1]

Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States); University of California, Berkeley, CA (United States)
Univ. of Washington, Seattle, WA (United States); Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Non-invasive methods of detecting radiation exposure show promise to improve upon current approaches to biological dosimetry in ease, speed, and accuracy. Here we developed a pipeline that employs Fourier transform infrared (FTIR) spectroscopy in the mid-infrared spectrum to identify a signature of low dose ionizing radiation exposure in mouse ear pinnae over time. Mice exposed to 0.1 to 2 Gy total body irradiation were repeatedly measured by FTIR at the stratum corneum of the ear pinnae. We found significant discriminative power for all doses and time-points out to 90 days after exposure. Classification accuracy was maximized when testing 14 days after exposure (specificity > 0.9 with a sensitivity threshold of 0.9) and dropped by roughly 30% sensitivity at 90 days. Infrared frequencies point towards biological changes in DNA conformation, lipid oxidation and accumulation and shifts in protein secondary structure. Since only hundreds of samples were used to learn the highly discriminative signature, developing human-relevant diagnostic capabilities is likely feasible and this non-invasive procedure points toward rapid, non-invasive, and reagent-free biodosimetry applications at population scales.

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Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 2470989

Journal Information:: Scientific Reports, Journal Name: Scientific Reports Journal Issue: 1 Vol. 14; ISSN 2045-2322

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

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Title: Long-term, non-invasive FTIR detection of low-dose ionizing radiation exposure

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