Robust, versatile DNA FISH probes for chromosome-specific repeats in Caenorhabditis elegans and Pristionchus pacificus
[1];
[2]
- University of California, Berkeley, CA (United States)
- University of California, Berkeley, CA (United States); Howard Hughes Medical Institute, Chevy Chase, MD (United States); Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States); California Institute for Quantitative Biosciences, Berkeley, CA (United States)
Repetitive DNA sequences are useful targets for chromosomal fluorescence in situ hybridization. We analyzed recent genome assemblies of Caenorhabditis elegans and Pristionchus pacificus to identify tandem repeats with a unique genomic localization. Based on these findings, we designed and validated sets of oligonucleotide probes for each species targeting at least 1 locus per chromosome. These probes yielded reliable fluorescent signals in different tissues and can easily be combined with the immunolocalization of cellular proteins. Synthesis and labeling of these probes are highly cost-effective and require no hands-on labor. The methods presented here can be easily applied in other model and nonmodel organisms with a sequenced genome.
- Research Organization:
- Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
- Sponsoring Organization:
- USDOE
- Grant/Contract Number:
- AC02-05CH11231
- OSTI ID:
- 2470818
- Journal Information:
- G3, Journal Name: G3 Journal Issue: 7 Vol. 12; ISSN 2160-1836
- Publisher:
- Genetics Society of AmericaCopyright Statement
- Country of Publication:
- United States
- Language:
- English
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