Cryo-EM structure of the Rhodospirillum rubrum RC–LH1 complex at 2.5 Å

Qian, Pu; Croll, Tristan I.; Swainsbury, David J.K.; Castro-Hartmann, Pablo; Moriarty, Nigel W.; Sader, Kasim; Hunter, C. Neil

doi:10.1042/bcj20210511

Title: Cryo-EM structure of the Rhodospirillum rubrum RC–LH1 complex at 2.5 Å

Journal Article · Tue Sep 07 00:00:00 UTC 2021 · Biochemical Journal

DOI: https://doi.org/10.1042/bcj20210511 · OSTI ID:2470713

Qian, Pu ^[1]; Croll, Tristan I. ^[2]; Swainsbury, David J.K. ^[3]; Castro-Hartmann, Pablo ^[4]; Moriarty, Nigel W. ^[5]; Sader, Kasim ^[4];

^[3]

Thermo Fisher Scientific, Eindhoven (Netherlands); University of Sheffield (United Kingdom)
University of Cambridge (United Kingdom)
University of Sheffield (United Kingdom)
Thermo Fisher Scientific, Eindhoven (Netherlands)
Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

The reaction centre light-harvesting 1 (RC–LH1) complex is the core functional component of bacterial photosynthesis. We determined the cryo-electron microscopy (cryo-EM) structure of the RC–LH1 complex from Rhodospirillum rubrum at 2.5 Å resolution, which reveals a unique monomeric bacteriochlorophyll with a phospholipid ligand in the gap between the RC and LH1 complexes. The LH1 complex comprises a circular array of 16 αβ-polypeptide subunits that completely surrounds the RC, with a preferential binding site for a quinone, designated Q_P, on the inner face of the encircling LH1 complex. Quinols, initially generated at the RC Q_B site, are proposed to transiently occupy the Q_P site prior to traversing the LH1 barrier and diffusing to the cytochrome bc₁ complex. Thus, the Q_P site, which is analogous to other such sites in recent cryo-EM structures of RC–LH1 complexes, likely reflects a general mechanism for exporting quinols from the RC–LH1 complex.

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Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: Biotechnology and Biological Sciences Research Council (BBSRC); European Research Council Synergy Award; USDOE; Wellcome Trust grant

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 2470713

Journal Information:: Biochemical Journal, Journal Name: Biochemical Journal Journal Issue: 17 Vol. 478; ISSN 0264-6021

Publisher:: Biochemical SocietyCopyright Statement

Country of Publication:: United States

Language:: English

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Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
59 BASIC BIOLOGICAL SCIENCES
biochemistry & molecular biology
bioenergetics
carotenoids
cryo-electron microscopy
light-harvesting
photosynthesis
quinone
reaction centre
structural biology

Title: Cryo-EM structure of the Rhodospirillum rubrum RC–LH1 complex at 2.5 Å

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