A scalable and tunable thermoreversible polymer for 3D human pluripotent stem cell biomanufacturing

Johnson, Hunter J.; Chakraborty, Saheli; Muckom, Riya J.; Balsara, Nitash P.; Schaffer, David V.

doi:10.1016/j.isci.2022.104971

Title: A scalable and tunable thermoreversible polymer for 3D human pluripotent stem cell biomanufacturing

Journal Article · 25 August 2022 · iScience

DOI: https://doi.org/10.1016/j.isci.2022.104971 · OSTI ID:2470268

Johnson, Hunter J. ^[1]; Chakraborty, Saheli ^[2];

^[3]; Balsara, Nitash P. ^[4]; Schaffer, David V. ^[4]

University of California, Berkeley, CA (United States); Univ. of California, San Francisco, CA (United States)
Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States); University of California, Berkeley, CA (United States)
University of California, Berkeley, CA (United States)

Human pluripotent stem cells (hPSCs) are an exciting and promising source to enable cell replacement therapies for a variety of unmet medical needs. Though hPSCs can be successfully derived into numerous physiologically relevant cell types, effective translation to the clinic is limited by challenges in scalable production of high-quality cells, cellular immaturity following the differentiation process, and the use of animal-derived components in culture. To address these limitations, we have developed a fully defined, reproducible, and tunable thermoreversible polymer for high-quality, scalable 3D cell production. Our reproducible synthesis method enables precise control of gelation temperature (24°C–32°C), hydrogel stiffness (100–4000 Pa), and the prevention of any unintended covalent crosslinking. After material optimization, we demonstrated hPSC expansion, pluripotency maintenance, and differentiation into numerous lineages within the hydrogel. Overall, this 3D thermoreversible hydrogel platform has broad applications in scalable, high-quality cell production to overcome the biomanufacturing burden of stem cell therapy.

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Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States). Joint Center for Energy Storage Research (JCESR)

Sponsoring Organization:: USDOE

Grant/Contract Number:: AC02-06CH11357

OSTI ID:: 2470268

Journal Information:: iScience, Journal Name: iScience Journal Issue: 10 Vol. 25; ISSN 2589-0042

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

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