Structural basis of DNA recognition of the Campylobacter jejuni CosR regulator

Zhang, Zhemin; Yan, Yuqi; Pang, Jinji; Dai, Lei; Zhang, Qijing; Yu, Edward W.

doi:10.1128/mbio.03430-23

Title: Structural basis of DNA recognition of the Campylobacter jejuni CosR regulator

Journal Article · 07 February 2024 · mBio (Online)

DOI: https://doi.org/10.1128/mbio.03430-23 · OSTI ID:2470051

Zhang, Zhemin ^[1]; Yan, Yuqi ^[1]; Pang, Jinji ^[2]; Dai, Lei ^[2]; Zhang, Qijing ^[2];

^[1]

Case Western Reserve University School of Medicine, Cleveland, OH United States)
Iowa State University, Ames, IA (United States)

Campylobacter jejuni is a foodborne pathogen commonly found in the intestinal tracts of animals. This pathogen is a leading cause of gastroenteritis in humans. Besides its highly infectious nature, C. jejuni is increasingly resistant to a number of clinically administrated antibiotics. As a consequence, the Centers for Disease Control and Prevention has designated antibiotic-resistant Campylobacter as a serious antibiotic resistance threat in the United States. The C. jejuni CosR regulator is essential to the viability of this bacterium and is responsible for regulating the expression of a number of oxidative stress defense enzymes. Importantly, it also modulates the expression of the CmeABC multidrug efflux system, the most predominant and clinically important system in C. jejuni that mediates resistance to multiple antimicrobials. Here, we report structures of apo-CosR and CosR bound with a 21 bp DNA sequence located at the cmeABC promotor region using both single-particle cryo-electron microscopy and X-ray crystallography. These structures allow us to propose a novel mechanism for CosR regulation that involves a long-distance conformational coupling and rearrangement of the secondary structural elements of the regulator to bind target DNA.

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Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States). Advanced Photon Source (APS)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES). Scientific User Facilities (SUF); National Institutes of Health (NIH)

Grant/Contract Number:: AC02-06CH11357

OSTI ID:: 2470051

Journal Information:: mBio (Online), Journal Name: mBio (Online) Journal Issue: 3 Vol. 15; ISSN 2150-7511

Publisher:: American Society for Microbiology (ASM)Copyright Statement

Country of Publication:: United States

Language:: English

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CosR-DNA complex
59 BASIC BIOLOGICAL SCIENCES
Campylobacter jejuni
CosR
Microbiology
X-ray crystallography
cryo-electron microscopy
multidrug resistance
oxidative stress

Title: Structural basis of DNA recognition of the Campylobacter jejuni CosR regulator

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